Radiosensitivity and TP53, EGFR Amplification and LOH10 Analysis of Primary Glioma Cell Cultures

Abstract

In this small series of early passage glioma cell cultures low radiosensitivity and alterations in cell regulatory genes were seen. Further testing of biological behavior in larger series of patient-derived material is ongoing.

Expression of p53 and p21 in Primary Glioblastomas

“…These findings are similar to our results for p53 protein in vivo. Nevertheless, genetic alterations within the p53-p21 pathway in vitro do correlate with reduced radiosensitivity in some cases, as shown in this study and other reports [1,8,40], but the mechanisms causing radioresistance still remain unclear. Therefore, we wanted to elucidate these mechanisms further and investigated GBM cells in vitro.…”

“…Primary GBMs, however, are equally radioresistant [2,8,22], a fact not easily explicable on the grounds of their known genetic alterations. An intriguing finding in approximately 30% of primary GBMs is an accumulation of wild-type (wt) p53 protein [35].…”

Correlation analysis between the expression of P21WAF1/CIP1, P16 proteins and human glioma

“…Genetic alterations in malignant gliomas, as visualised by cytogenetics, are extremely complex and diverse. There are two important molecular pathways: P53 pathway and Rb pathway, which show frequent alterations and seem to be pathogenetically relevant [13][14][15].…”

Early Effects of Irradiation on [123I]-IMT and [18F]-FDG Uptake in Rat C6 Glioma Cells

“…It is well known that many parameters such as radiation dose and fractionation, individual tumor radiosensitivity, oxidative status and vascular supply have to be taken into account when monitoring the effects of radiotherapy in the in vivo situation [26,36]. However, in vitro models offer the advantage of studying the influence of single parameters in a standardized and less complex tumor model [11].…”