Radioprotective properties of apple polyphenols: An in vitro study

Abstract

Present study was undertaken to evaluate the radioprotective ability of total polyphenols extracted from edible portion (epicarp and mesocarp) of apple. Prior administration of apple polyphenols to murine thymocytes significantly countered radiation induced DNA damage (evaluated by alkaline halo assay) and cell death (trypan blue exclusion method) in a dose dependent manner maximally at a concentration of 2 and 0.2 mg/ml respectively. Apple polyphenols in a dose dependent fashion inhibited both radiation or Fenton reaction mediated 2-deoxyribose (2-DR) degradation indicating its ability to scavenge hydroxyl radicals and this activity was found to be unaltered in presence of simulated gastric juice. Similarly apple polyphenols in a dose dependent fashion scavenged DPPH radicals (maximum 69% at 1 mg/ml), superoxide anions (maximum 88% at 2 mg/ml), reduced Fe(3 +) to Fe(2 +) (maximum at 1 mg/ml) and inhibited Fenton reaction mediated lipid peroxidation (maximum 66% at 1.5 mg/ml) further establishing its antioxidative properties. Studies carried out with plasmid DNA revealed the ability of apple polyphenols to inhibit radiation induced single as well as double strand breaks. The results clearly indicate that apple polyphenols have significant potential to protect cellular system from radiation induced damage and ability to scavenge free radicals might be playing an important role in its radioprotective manifestation.

Evaluation of Radioprotective Effects of Propolis and Quercetin on Human White Blood Cells in Vitro

“…Results obtained in these studies indicate both the beneficial effects of tested substances as well as sensitivity of the assay. [32][33][34][35][36][37][38][39] The results of present alkaline comet study indicate favourable toxicity profiles of EEP and quercetin. Both compounds offered acceptable radioprotection in vitro when weighed against well-known chemical radioprotector AET.…”

Radioprotection by quercetin-3-O-rutinoside, a flavonoid glycoside – A cellular and mechanistic approach

“…Several plant products have also been employed for mitigating the ionizing radiation-induced damage in mammalian systems (Arora et al, 2005;Paul, Unnikrishnan, & Nagappa, 2011). This is owing to the polyphenolic constitution of plant material, among which flavonoids are most abundant and responsible in attenuating the radiation-induced oxidative distress (Benkovic et al, 2008;Chaudhary et al, 2006;Devi, Bisht, & Vinitha, 1998;Hosseinimehr, Tavakoli, Pourheidari, Sobhani, & Shafiee, 2003;Kalpana et al, 2011). In our laboratory, we have screened a few plants, e.g.…”

Radioprotective Effects of Quercetin and Ethanolic Extract of Propolis in Gamma-Irradiated Mice

“…These include polyphenols and honeybee products such as propolis and bee venom. The awareness of their radioprotective properties has increased over the last decade, and their effects have extensively been studied in vitro and in vivo (5)(6)(7)(8)(9)(10)(11)(12). Polyphenols are abundant micronutrients in human diet and include hydroxybenzoic acids, hydroxycinnamic acids, anthocyanins, proanthocyanidins, fl avonols, fl avones, fl avanols, fl avanones, isofl avones, stilbenes, and lignans (13).…”