Radioprotective Effect of Panax ginseng on the Phosphatases and Lipid Peroxidation Level in Testes of Swiss Albino Mice.

Abstract

The Panax ginseng has been used as traditional medicine for past several years among oriental people. The present investigation has been made to assess the radioprotective efficacy of ginseng root extract in the testicular enzymes of Swiss albino mice. The Swiss albino mice were divided into different groups. (i) Ginseng treated group: The animals were administered 10 mg/kg body weight ginseng root extract i.p. (ii) Radiation treated group: The animals were exposed to 8 Gy gamma radiation at the dose rate of 1.69 Gy/min at the distance of 80 cm. (iii) Combination group: Animals were administered ginseng extract continuously for 4 d and on 4th day they were irradiated to 8 Gy gamma radiation after 30 min of extract administration. The animals from above groups were autopsied on day 1, 3, 7, 14 and 30. Biochemical estimations of acid and alkaline phosphatases and Lipid peroxidation (LPO) in testes were done. In ginseng treated group acid and alkaline phosphatases activity and LPO level did not show any significant alteration. In irradiated animals there was a significant increase in acid phosphatase activity and LPO level. However, significant decline in alkaline phosphatase activity was observed. The treatment of ginseng before irradiation causes significant decrease in acid phosphatase and LPO level and significant increase in alkaline phosphatase activity. One of the cause of radiation damage is lipid peroxidation. Due to lipid peroxidation, lysosomal membrane permeability alters and thus results in release of hydrolytic enzymes. So, an increase in acid phosphatase was noticed after radiation treatment. The alkaline phosphatase activity is associated with membrane permeability and different stages of spermatogenesis. Due to membrane damage and depletion of germ cells of testes after irradiation the enzyme activity was decreased. Ginseng markedly inhibits lipid peroxidation. It acts in indirect fashion to protect radical processes by inhibition of initiation of free radical processes and thus reduces the radiation damages in testes of Swiss albino mice.

Protective Role of Some Feed Additives against Dizocelpine Induced Oxidative Stress in Testes of Rabbit Bucks

“…The results are agreement with those of Sawiress et al (2011) who showed that rats under oxidative stress had reduced alkaline phosphatase, gamma glutamyl transferase and lactate dehydrogenase activities. Within the testis, the ALP activity was of significant importance for the process of spermatogenesis, moreover, the decrease in its activity was correlated with the state of germ cell loss (Kumar et al, 2003). The present investigation showed that rabbits subjected to dizocilipine oxidative stress resulted in decrease in ALP activities which correlated with reduction in testicular weights, and sperm counts together with the pathological findings.…”

Antioxidative properties of traditional herbal medicines and the application of comet assay on antioxidative study

“…Radioprotective efficacy of ginseng root extract in the testicular enzymes of Swiss albino mice has been assessed (Kumar et al, 2003). Levels of acid and alkaline phosphatases and lipid peroxidation (LPO) in testes were estimated on ginseng treated, radiation treated and combination groups.…”

Flavonoids of Rosa roxburghii Tratt Act as Radioprotectors

“…People have isolated a series of active ingredients from natural anti-radiation substances, such as polysaccharides (Subramanian et al, 2002;Song et al, 2003;Il´in et al, 2004;Fan et al, 2005) (Devi et al, 1998;Liu et al, 2002;Zielonka et al, 2003;, polyphenols (Hibasami et al, 2000;Lv et al, 2004), saponins (Chen et al, 1999;Kim et al, 2001;Li et al, 2001;Kuinar et al, 2003), alkaloids (Liang et al, 2002;Makarchenko and Utkina 2006 ), and peptides compounds (Zhu and Zhong 2004;Yang 2007), etc. Rosa roxburghii Tratt (Figure 1) is the fruit of jiao-si flower or unicuspid jiao-si flower from rosaceae, which was firstly recorded in the "ben-cao-gang-mu-shi-yi".…”