Radioprotective Effect of Human Lactoferrin Against Gamma-Irradiation With Sublethal Dose

Kopaeva, Marina Yu.; Алчинова, И. Б.; Nesterenko, Mikhail; Cherepov, Anton; Demorzhi, M. S.; Zarayskaya, Irina Yu.; Karganov, Mikhail Yu.

doi:10.21175/radproc.2020.09

Cited by 2 publications

(2 citation statements)

References 8 publications

(13 reference statements)

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“…In 24 h after irradiation, the mice were re-injected with hLf (groups IR+Lf×2, AC+Lf×2) or saline (groups IR+Lf, AC+Lf). The dose of Lf was chosen based on published data [ 4 ], results of our previous studies [ 32 , 33 ], and pilot experiments [ 34 ]. The animals in the IR and AC groups were twice injected with saline in the same way.…”

Section: Methodsmentioning

confidence: 99%

New Properties of a Well-Known Antioxidant: Pleiotropic Effects of Human Lactoferrin in Mice Exposed to Gamma Irradiation in a Sublethal Dose

Kopaeva

Алчинова²,

Cherepov³

et al. 2022

Antioxidants

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We studied the effects of human lactoferrin (hLf), a multifunctional protein from the transferrin family, on integral (survival, lifespan during the experiment, body weight, behavior, subfractional compositions of blood serum) and systemic (hemoglobin level, leukocyte number, differential leukocyte count, histological structure of the liver and spleen) parameters of the body in mice after acute gamma irradiation in a sublethal dose. The experiments were performed on male C57BL/6 mice. The mice in the experimental groups were exposed to whole-body gamma radiation in a dose of 7.5 Gy from a 60Co source. Immediately after irradiation and 24 h after it, some animals received an intraperitoneal injection of hLf (4 mg/mouse). Single or repeated administration of hLf had a positive pleiotropic effect on irradiated animals: animal survival increased from 28% to 78%, and the mean life expectancy during the experiment (30 days) increased from 16 to 26 days. A compensatory effect of hLf on radiation-induced body weight loss, changes in homeostasis parameters, and a protective effect on the structural organization of the spleen were demonstrated. These data indicate that Lf has potential as a means of early therapy after radiation exposure.

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Section: Methodsmentioning

confidence: 99%

New Properties of a Well-Known Antioxidant: Pleiotropic Effects of Human Lactoferrin in Mice Exposed to Gamma Irradiation in a Sublethal Dose

Kopaeva

Алчинова²,

Cherepov³

et al. 2022

Antioxidants

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“…введение Лактоферрин (Лф) -полифункциональный белок из семейства трансферринов. Он активно исследуется в экспериментальных патологических моделях заболеваний человека в качестве антиаллергического, иммуномодулирующего, радиопротекторного агента [1,2], белка, способного ослабить прогрессирование нейродегенеративных заболеваний и стимулировать нейрорегенерацию [3]. Особый интерес вызывает способность Лф модулировать широкий спектр нейрональных процессов посредством изменения экспрессии генов, участвующих в долговременной нейропластичности (BDNF -Brain-Derived Neurotrophic Factor; CREB -cAMP Response Element-Binding protein, транскрипционный фактор; CAMK -Ca 2+ /calmodulin-dependent protein kinase, и др.)…”

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Human lactoferrin enhances the expression of transcription factor c-Fos in neuronal cultures under stimulated conditions

Копаева¹,

Азиева²,

Cherepov³

et al. 2021

Nauchno-Prakticheskii Zhurnal «Patogenez»

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Целью настоящей работы стало исследование влияния лактоферрина (Лф) человека на экспрессию транскрипционного фактора c-Fos в первичных нейрональных культурах после физиологической стимуляции, определение клеточной локализации Лф человека и возможной колокализации экзогенного белка с индуцированной экспрессией c-Fos. Методы. Первичные диссоциированные клеточные культуры получали из гиппокампа головного мозга новорожденных мышей (Р0-Р1) линии С57Вl/6. Индукцию экспрессии белка c-Fos в клетках осуществляли путем трехкратного добавления 50 мМ KСl в культуральную среду на 8-й день культивирования in vitro. Анализ содержания c-Fos проводили иммунофлюоресцентным методом через 2 часа после стимуляции. Результаты. Лф детектировался как в цитоплазме, так и в ядрах отдельных клеток культуры после стимуляции KСl. В ядрах некоторых клеток была выявлена колокализация включения Лф и экспрессии c-Fos. Было обнаружено, что предварительное введение Лф в культуральную среду увеличивало количество клеток, экспрессирующих c-Fos после добавления 50 мМ KСl. The aims of this research were 1) to study the effect of human lactoferrin (Lf) on the expression of the c-Fos transcription factor in primary neuronal cultures after physiological stimulation; 2) to determine the cellular localization of human Lf and possible colocalization of an exogenous protein with induced c-Fos expression. Methods. Primary dissociated cell cultures were obtained from the hippocampus of newborn C57Bl/6 mice (P0-P1). The expression of c-Fos was induced by addition of 50 mM KCl to the culture medium at 8 day in vitro. c-Fos content was analyzed by immunofluorescence 2 hrs after stimulation. Results. Lf was detected in cytoplasm and in nuclei after stimulation KCl. Lf inclusion and c-Fos expression were colocalized in the nuclei of some cells. Thus, results showed that pretreatment with Lf led to increase in the number of cells expressing c-Fos after exposure to 50 mM KCl.

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Radioprotective Effect of Human Lactoferrin Against Gamma-Irradiation With Sublethal Dose

Cited by 2 publications

References 8 publications

New Properties of a Well-Known Antioxidant: Pleiotropic Effects of Human Lactoferrin in Mice Exposed to Gamma Irradiation in a Sublethal Dose

New Properties of a Well-Known Antioxidant: Pleiotropic Effects of Human Lactoferrin in Mice Exposed to Gamma Irradiation in a Sublethal Dose

Human lactoferrin enhances the expression of transcription factor c-Fos in neuronal cultures under stimulated conditions

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