Radioprotective effect of antioxidative flavonoids in γ-ray irradiated mice

Abstract

The anticlastogenic effect of 12 structurally different flavonoids was investigated in whole body gamma-ray irradiated mice. Each flavonoid was administered to ICR male mice by a single gastric intubation (5 mumol/kg) 6 h before gamma-ray irradiation (1.5 Gy) and the frequency of micronucleated reticulocytes (MNRETs) in peripheral blood was determined. In order to elucidate the mechanism of the anticlastogenic effect of these flavonoids, their antioxidative activities were examined by the thiobarbituric acid method using methyl linoleate and Fenton's reagent (Fe2+/H2O2). Of the 12 flavonoids, luteolin had the most marked effect on reducing the frequencies of MNRETs and also inhibiting lipid peroxidation. However, quercetin tetramethylether, which has methoxy groups instead of hydroxyl groups at the 3,7,3',4'-positions, and phloretin with an open C-ring showed the least anticlastogenic and antioxidative activity. A good correlation (r = 0.717, P < 0.01) was observed between the anticlastogenic activity and the antioxidative activity of the 12 flavonoids. These results suggest that the radioprotective effect of flavonoids in mice may be attributed to the hydroxyl radical scavenging potency in a direct or an endogenous enzyme mediated manner.

Phenylpropanoids in radioregulation: double edged sword

“…Genistein (6) (4',5,7-trihydroxyflavone), a naturally occurring isoflavone found in soybeans, has been reported to have protective effects against cellular damage such as UVB-induced oxidative stress and IR-induced damage (Shimoi et al, 1994;Wei et al, 2002). One study demonstrated that administration of genistein (6) resulted in alleviation of the lethal effects of radiation exposure in mice without changes in behavior, body weight or histopathology.…”

Effect of Luteolin on the Levels of Glycoproteins During Azoxymethane-induced Colon Carcinogenesis in Mice

“…Luteolin has antiallergic properties (Kimata et al, 2000a), and luteolin acts as a potent inhibitor of human mast cell activation through the inhibition of protein kinase C activation and Ca 2+ influx (Kimata et al, 2000b). It is also reported to have anti-inflammatory/anti-allergic (Veda et al, 2002), anti-tumorigenic (Yasukawa et al, 1989) and antioxidant properties (Shimoi et al, 1994).…”

Biological activity and mechanisms of therapeutic action of rosmarinic acid, luteolin and its sulphated derivatives

“…Как ароматическое соединение, ЛТ может поддерживать неспаренные электроны вокруг М-электронной системы. Прямое доказательство того, что ЛТ, является перехватчиком АФК, было получено в бесклеточных системах [25].…”