Radioprotection of the mice parotid gland by isoproterenol: study on morphometry of secretory granules and on autoradiography

Onodera, T; Nasu, Masanori; Iwata, Hiroshi; Yosue, Takashi

doi:10.1007/s11282-006-0037-8

Cited by 4 publications

(3 citation statements)

References 45 publications

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“…After 30 days of irradiation, the mice in groups A, B, D, and E were administered an intraabdominal injection of 370 kBq/g of 3 H-leucine (L-leucine-4,5-3 H, 2.22 -3.33 TBq (60-90 Ci)/mmol ; Moravek Biochemicals, Inc., USA) (hereafter referred to as RI administration). The mice in each of the abovementioned groups were sacrificed under deep anesthesia at 30 and 120 min after RI administration, because in LMARG, the reduced silver grains in the acinar cells of the parotid gland show a maximum value at 30 min after 3 H-leucine administration and thereafter decreases with time 11 . The parotid glands were enucleated and their central portion was cut into fine particles of approximately 1 mm 3 in volume.…”

Section: Light Microscope Autoradiography (Lmarg)mentioning

confidence: 99%

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Effect of Preliminary Amifostine Administration in Irradiation of Parotid Glands

Yachi

Nasu

Yosue

2008

Oral Science International

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To investigate the effect of amifostine, we administered amifostine, a radioprotective agent, 30 minutes before exposing the maxillary region of mice, including the parotid gland, to 5 Gy or 10 Gy X‐ray irradiation. The survival rate was recorded, and changes in the parotid gland morphology were investigated by examining the hematoxylin and eosin (HE)‐stained specimens and light microscope autoradiography (LMARG) images obtained 30 days after irradiation. A survival rate of 100% was not observed in any group administered 200 mg/kg amifostine with or without irradiation. Among the groups irradiated with 10 Gy X‐rays, the survival rate was higher and the survival period was longer in the 100 mg/kg amifostine group than in the no amifostine group. The histological findings in the group that received 5 Gy irradiation without amifostine were as follows : auxetic growth of acinar cells, nuclei of all sizes, cells in the mitotic phase, and cells undergoing apoptosis. Further, the treated groups were compared with the no amifostine and no irradiation group (untreated control). LMRG imaging revealed that the number of reduced silver grains per mm2 of acinar cells after 30 min of 3H‐leucine administration was higher than that after 120 min in mice treated with 100 mg/kg amifostine with or without 5 Gy irradiation. This observation was similar to that in the untreated control. This finding suggests that although amifostine administration reduces the adverse effects of irradiation on the parotid gland, higher doses of amifostine may be fatal.

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Section: Light Microscope Autoradiography (Lmarg)mentioning

confidence: 99%

“…Onodera et al 11 sequentially observed the leucine absorption ability of the acinar cells of the mouse parotid gland by using LMARG. The amount of leucine in the acinar cells of the parotid gland was highest at 30 min after RI administration ( 3 H-leucine was injected intraabdominally into the mice).…”

Section: Leucine Quantity In Acinar Cellsmentioning

confidence: 99%

Effect of Preliminary Amifostine Administration in Irradiation of Parotid Glands

Yachi

Nasu

Yosue

2008

Oral Science International

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“…In addition, many studies have investigated the administration of cevimeline in a murine model of SS, MRL/l mice, which show spontaneous autoim-mune abnormalities [4][5][6], and resulted in dose-dependent increases in saliva secretion [7]. Similarly, the effects of cevimeline on saliva secretion have been investigated in rats and dogs [8,9], and the ultrastructure of the salivary glands of rats and mice have also been reported [10][11][12]. However, there are no reports describing the morphological changes in the salivary gland cells of cevimeline-treated SS model mice.…”

Section: Introductionmentioning

confidence: 99%

Effects of Cevimeline on the Immunolocalization of Aquaporin-5 and the Ultrastructure of Salivary Glands in Sjögren’s Syndrome Model Mice

et al. 2009

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Summary: Sjögren's syndrome (SS) is an autoimmune disorder whose main symptoms include xerostomia and dry eyes. It has been demonstrated that abnormal expression of aquaporin (AQP)-5 in the parotid and submandibular glands in SS model mice was corrected by cevimeline. In the present study, we orally administered cevimeline hydrochloride (cevimeline) to female MRL/l mice, which are widely used as a model for SS, to immunohistochemically investigate the localization of AQP-5 in the salivary glands. We also assessed the ultrastructure of acinar cells in the submandibular glands. AQP-5 was expressed in the apical and lateral cell membranes of acinar cells in the parotid and submandibular glands of normal mice, but not in the sublingual glands. In contrast, AQP-5 was expressed not only in the cell membranes in the apical domains but also in the cytoplasm in the SS model mice, indicating that the localization of AQP-5 was disordered in the SS model mice. After administration of cevimeline, AQP-5 was predominantly localized in the cellular apical domains of the acinar cells. Electron microscopy revealed that administration of cevimeline to the SS model mice and normal mice markedly reduced the number of secretory granules, increased the area of the rough endoplasmic reticulum, and expanded the intercellular gaps in the cells of the submandibular acini. Condensed vacuoles were also observed in the Golgi apparatuses, indicating that secondary enhancement of secretion and production of saliva had occurred. Consequently, the results of the present study demonstrate that the administration of cevimeline to the SS model mice increased salivary secretion in the submandibular glands. Furthermore, cevimeline transiently normalized the localization of AQP-5 expression in the parotid and submandibular glands.

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Possible radioprotection of submandibular glands in gamma-irradiated rats using kaempferol: a histopathological and immunohistochemical study

Ahmed

Bakr

Abdelgawad

et al. 2022

International Journal of Radiation Biology

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Radioprotection of the mice parotid gland by isoproterenol: study on morphometry of secretory granules and on autoradiography

Cited by 4 publications

References 45 publications

Effect of Preliminary Amifostine Administration in Irradiation of Parotid Glands

Effect of Preliminary Amifostine Administration in Irradiation of Parotid Glands

Effects of Cevimeline on the Immunolocalization of Aquaporin-5 and the Ultrastructure of Salivary Glands in Sjögren’s Syndrome Model Mice

Possible radioprotection of submandibular glands in gamma-irradiated rats using kaempferol: a histopathological and immunohistochemical study

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