Radioprotection of Mice by the Bacterial Extract Broncho-Vaxom<sup>R</sup>: Haemopoietic Stem Cells and Survival Enhancement

Fedoročko, Peter; Brezáni, P; Macková, N

doi:10.1080/09553009214551271

Cited by 17 publications

(8 citation statements)

References 18 publications

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“…This process may thus be one of the mechanisms leading to earlier hemopoietic recovery after irradiation (26,27). BV-mediated radioprotection has been demonstrated to accelerate the regeneration of not only peripheral blood cells but also endoCFU-S and femoral CFU-S and GM-CFC (11,13).…”

Section: Discussionmentioning

confidence: 99%

“…Our previous studies demonstrated that BV injection before irradiation increased the number of endogenous colony-forming units (endoCFU-S) in Combined radiopro tec tion sublethally irradiated mice and increased the number of mice that survived beyond 30 days after lethal irradiation (1 1, 12). Also, BV-injection accelerated early recovery of hemopoietic stem cells and cells in the peripheral blood (13,14).…”

Section: Introductionmentioning

confidence: 99%

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Radioprotective effects of combination broncho‐vaxom, a macrophage activator, and indomethacin, an inhibitor of prostaglandin production: relationship to myelopoiesis

Fedoročko

Macková

1996

European J of Haematology

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The effects of the bacterial extract broncho‐vaxom (BV; radioprotective immunomodulator; 500 μg/mouse i.p., 24 h) and indomethacin (INDO; inhibitor of prostaglandin production; 2 times 40 μg/mouse i.m., ‐ 24 h and ‐ 3 h) on the post‐irradiation recovery of hemopoietic functions in mice were investigated. Both agents were administered either alone or in combination. Endogenous spleen colony formation was increased in all treatment groups, with combination‐treated mice exhibiting the greatest effects. Similarly, 24 h after combined administration of BV and INDO (i.e. at the time of presumed irradiation) to the non‐irradiated mice granulocyte‐macrophage colony‐forming cell (GM‐CFC) numbers were greater in the bone marrow and spleen. Also, as determined by hydroxyurea injection, there was an increase in the number of GM‐CFC in the S‐phase of the cell cycle in the bone marrow. However, GM‐CFC in the spleen of combination pretreated mice was not stimulated to significant proliferation as compared to GM‐CFC in the spleen of mice injected with BV alone. Combined modality treatment was also more effective than single agent treatments in accelerating bone marrow cellularity and GM‐CFC regeneration, but not in accelerating GM‐CFC regeneration in the spleen. Combined administration of BV and INDO to mice prior to lethal irradiation exerted an additional radioprotective effect and protected 95% of the C57B1/6 mice.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Radioprotective effects of combination broncho‐vaxom, a macrophage activator, and indomethacin, an inhibitor of prostaglandin production: relationship to myelopoiesis

Fedoročko

Macková

1996

European J of Haematology

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“…OM-85 BV has re cently been shown to stimulate hemopoietic cell survival in irradiated animals [25]. Indeed, intraperitoneal injec tion of mice with OM-85 BV before irradiation (1-10.5 Gy of y-rays) induced a highly significant, dose-and timedependent increase of hemopoietic stem cells relative to control (saline-injected) animals, as determined from the number of endogenous spleen colonies 10 days after irra diation ( fig.…”

Section: Effect O F Om-85 Bv On the Hemopoietic Systemmentioning

confidence: 99%

“…Macrophages from C57BL/6 mice were exposed to OM-85 BV for 24 h. Nitrite production was then measured spectrophotometrically in cell supemates using the Griess reagent [25]; HMPS activity was tested in parallel as indicated in the legend to figure 1. Data from ref.…”

Section: Cytotoxic Activities O F Macrophagesmentioning

confidence: 99%

Stimulation of Immunoprotective Mechanisms by OM-85 BV

Mauël

1994

Respiration

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OM-85 BV, an immunomodulating preparation containing extracts from eight commonly pathogenic bacterial species, has been used with success as an oral adjuvant in the prevention of respiratory tract infections. Results from in vitro and in vivo experiments suggest various mechanisms that can underlie this beneficial effect. Thus, exposure of murine macrophages in vitro to OM-85 BV led to stimulation of biochemical and functional parameters associated with the disposal of microorganisms and tumor cells. Similarly, blood-derived human phagocytes were stimulated to express adhesion molecules (LFA-1, MAC-1, pl50,95, ICAM-1), to synthesize TNF-α and IL-2, and to develop a natural killer activity. These in vitro functions are reflected in the activation of immunological processes in vivo following administration of OM-85 BV per os. Oral treatment of mice and rabbits increased the capacity of the animals to clear bacteria from the blood, an effect that could be ascribed to enhanced functional activity of polymorphonuclear leukocytes. Administration of OM-85 BV per os also led to enhanced salivary IgA levels in man, and in gut and lung secretions in animals. Stimulation of migration and the beneficial effects of OM-85 BV correlated with phagocytosis-induced superoxide production in human bronchoalveolar lavage cells from orally treated individuals. Finally, injection of OM-85 BV was shown to enhance recovery from irradiation in animals, presumably by improving hemopoietic recovery. These findings indicate that OM-85 BV is capable of stimulating both cellular and humoral components of the immune response. In particular, administration per os promotes immune protective mechanisms active at the level of mucosal surfaces, thus providing a rationale for the oral prevention of acute exacerbations of chronic and recurrent respiratory tract infections using bacteria-derived immunomodulating agents.

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“…Broncho-Vaxom ® is a bacterial lysate. It has been shown to significantly enhance post-irradiation survival in several mouse strains [ 55 ]. Subsequent studies have revealed positive hematological effects of Broncho-Vaxom ® in sublethally irradiated mice [ 56 , 57 , 58 ].…”

Section: Immunomodulatorsmentioning

confidence: 99%

Pharmacological Modulation of Radiation Damage. Does It Exist a Chance for Other Substances than Hematopoietic Growth Factors and Cytokines?

Höfer

Hoferová

Falk

2017

IJMS

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In recent times, cytokines and hematopoietic growth factors have been at the center of attention for many researchers trying to establish pharmacological therapeutic procedures for the treatment of radiation accident victims. Two granulocyte colony-stimulating factor-based radiation countermeasures have been approved for the treatment of the hematopoietic acute radiation syndrome. However, at the same time, many different substances with varying effects have been tested in animal studies as potential radioprotectors and mitigators of radiation damage. A wide spectrum of these substances has been studied, comprising various immunomodulators, prostaglandins, inhibitors of prostaglandin synthesis, agonists of adenosine cell receptors, herbal extracts, flavonoids, vitamins, and others. These agents are often effective, relatively non-toxic, and cheap. This review summarizes the results of animal experiments, which show the potential for some of these untraditional or new radiation countermeasures to become a part of therapeutic procedures applicable in patients with the acute radiation syndrome. The authors consider β-glucan, 5-AED (5-androstenediol), meloxicam, γ-tocotrienol, genistein, IB-MECA (N6-(3-iodobezyl)adenosine-5’-N-methyluronamide), Ex-RAD (4-carboxystyryl-4-chlorobenzylsulfone), and entolimod the most promising agents, with regards to their contingent use in clinical practice.

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Radioprotection of Mice by the Bacterial Extract Broncho-Vaxom^R: Haemopoietic Stem Cells and Survival Enhancement

Cited by 17 publications

References 18 publications

Radioprotective effects of combination broncho‐vaxom, a macrophage activator, and indomethacin, an inhibitor of prostaglandin production: relationship to myelopoiesis

Radioprotective effects of combination broncho‐vaxom, a macrophage activator, and indomethacin, an inhibitor of prostaglandin production: relationship to myelopoiesis

Stimulation of Immunoprotective Mechanisms by OM-85 BV

Pharmacological Modulation of Radiation Damage. Does It Exist a Chance for Other Substances than Hematopoietic Growth Factors and Cytokines?

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Radioprotection of Mice by the Bacterial Extract Broncho-VaxomR: Haemopoietic Stem Cells and Survival Enhancement

Cited by 17 publications

References 18 publications

Radioprotective effects of combination broncho‐vaxom, a macrophage activator, and indomethacin, an inhibitor of prostaglandin production: relationship to myelopoiesis

Radioprotective effects of combination broncho‐vaxom, a macrophage activator, and indomethacin, an inhibitor of prostaglandin production: relationship to myelopoiesis

Stimulation of Immunoprotective Mechanisms by OM-85 BV

Pharmacological Modulation of Radiation Damage. Does It Exist a Chance for Other Substances than Hematopoietic Growth Factors and Cytokines?

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Radioprotection of Mice by the Bacterial Extract Broncho-Vaxom^R: Haemopoietic Stem Cells and Survival Enhancement