Radiopaque scaffolds based on electrospun iodixanol/polycaprolactone fibrous composites

Perez, Joy Vanessa D.; Singhana, Burapol; Damasco, Jossana A.; Lu, Linfeng; Behlau, Paul; Rojo, Raniv D.; Whitley, Elizabeth M.; Heralde, Francisco M.; Melancon, Adam David; Huang, Steven Y.; Melancon, Marites P.

doi:10.1016/j.mtla.2020.100874

Cited by 6 publications

(8 citation statements)

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“…The copyright holder for this preprint this version posted November 24, 2022. ; https://doi.org/10.1101/2022.11.21.517369 doi: bioRxiv preprint scaffold increasing as much as 7-fold after 1 week of incubation. 23 In this study, fluorescence microscopy similarly revealed that the seeded Sprague-Dawley rat RFP-MSCs attached to the electrospun PCL scaffold (Figure 2).…”

Section: Electrospun Pcl Supported the Attachment Of Mscssupporting

confidence: 61%

“…We have shown in a previous study that an electrospun PCL scaffold can support the attachment of human GFP-expressing MSCs, with the number of viable cells attached to the scaffold increasing as much as 7-fold after 1 week of incubation. 23 In this study, fluorescence microscopy similarly revealed that the seeded Sprague-Dawley rat RFP-MSCs attached to the electrospun PCL scaffold (Figure 2).…”

Section: Electrospun Pcl Supported the Attachment Of Mscssupporting

confidence: 61%

“…22 We have previously shown that an electrospun PCL scaffold can support the attachment and proliferation of MSCs. 23 The objective of the current study was to determine the effects on NIH of PCL scaffolds with and without MSC attachment in a rat chronic kidney disease (CKD) model. Ultrasonography (US) is used clinically to assess parameters that predict AVF maturation, such as luminal diameter and flow rate.…”

Section: Introductionmentioning

confidence: 99%

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Bioresorbable Mesenchymal Stem Cell–Loaded Electrospun Polymeric Scaffold Inhibits Neointimal Hyperplasia Following Arteriovenous Fistula Formation in a Rat Model of Chronic Kidney Disease

Barcena

Perez

Bernardino

et al. 2022

Preprint

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In the setting of chronic kidney disease (CKD), the periadventitial injection of mesenchymal stem cells (MSCs) has shown significant potential in improving arteriovenous fistula (AVF) maturation by inhibiting neointimal hyperplasia (NIH). However, the rapid clearance of MSCs remains a challenge. Hence, we fabricated an electrospun perivascular scaffold from polycaprolactone (PCL) to support MSC attachment and allow gradual MSC elution at the outflow vein, the AVF site most prone to NIH. We performed 5/6th nephrectomy to induce CKD in Sprague-Dawley rats, followed by direct AVF formation and perivascular scaffold application. We then compared the following groups of CKD rats: no perivascular scaffold (i.e., control), PCL scaffold, and PCL+MSC scaffold. On ultrasonography, the PCL and PCL+MSC groups showed significantly reduced wall thickness and wall-to-lumen ratio and increased luminal diameter and flow rate. Of note, PCL+MSC group showed greater improvement in luminal diameter and flow rate compared to PCL alone. Moreover, 18F-fluorodeoxyglucose positron emission tomography showed that only PCL+MSC resulted in a significant reduction in uptake. On histology, the PCL and PCL+MSC groups showed significantly reduced neointima-to-lumen and neointima-to-media ratios and reduced neointimal CD45, α-SMA, and vimentin fluorescence staining compared to control. Although the two scaffold treatments did not differ significantly in histology, in vivo imaging suggested that addition of MSCs promoted greater luminal expansion and blood flow and reduced the inflammatory process underlying NIH. Our results demonstrate the utility of a mechanical support loaded with MSCs at the outflow vein immediately after AVF formation to support maturation by minimizing NIH.

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Section: Electrospun Pcl Supported the Attachment Of Mscssupporting

confidence: 61%

Section: Electrospun Pcl Supported the Attachment Of Mscssupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

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Bioresorbable Mesenchymal Stem Cell–Loaded Electrospun Polymeric Scaffold Inhibits Neointimal Hyperplasia Following Arteriovenous Fistula Formation in a Rat Model of Chronic Kidney Disease

Barcena

Perez

Bernardino

et al. 2022

Preprint

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“…It was tried to blend biodegradable polymer with an iodinated contrast agent, − yet the mechanical properties of the resultant composites are not satisfactory or not reported in pertinent literatures. In light of polymer physics, it is difficult to mix a hydrophilic iodinated contrast agent with a hydrophobic biodegradable polymer, especially when a high ratio of a contrast agent is needed for radiopacity in deep tissue.…”

Section: Introductionmentioning

confidence: 99%

A Facile Composite Strategy to Prepare a Biodegradable Polymer Based Radiopaque Raw Material for “Visualizable” Biomedical Implants

Wang

Chen

et al. 2022

ACS Appl. Mater. Interfaces

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Enabling a biodegradable polymer radiopaque under X-ray is much desired for many medical devices. Physical blending of a present biodegradable polymer and a commercialized medical contrast agent is convenient yet lacks comprehensive fundamental research. Herein, we prepared a biodegradable polymer-based radiopaque raw material by blending poly(L-lactic acid) (PLLA or simply PLA) and iohexol (IHX), where PLA constituted the continuous phase and IHX particles served as the dispersed phase. The strong X-ray adsorption of IHX enabled the composite radiopaque; the hydrolysis of the polyester and the water solubility of the contrast agent enabled the composite biodegradable in an aqueous medium. The idea was confirmed by in vitro characterizations of the resultant composite, in vivo subcutaneous implantation in rats up to 6 months, and the clear visualization of a part of a biodegradable occluder in a Bama piglet under X-ray. We also found that the crystallization of PLA was significantly enhanced in the presence of the solid particles, which should be taken into consideration in the design of an appropriate biomaterial composite because crystallization degree influences the biodegradation rate and mechanical property of a material to a large extent. We further tried to introduce a small amount of poly(vinylpyrrolidone) into the blend of PLA and IHX. Compared to the bicomponent composite, the tricomponent one exhibited decreased modulus and increased elongation at break and tensile strength. This paves more ways for researchers to select appropriate raw materials according to the regenerated tissue and the application site.

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“…12 Current clinically used CT contrast agents consist mainly of tri-iodinated benzene rings. 12 Our group has incorporated iodinated molecules in medical devices made of polycaprolactone 13 and poly(p-dioxanone), 14 resulting in significant radiopacity with a first-order signal degradation rate in CT when implanted in mice. However, iodine has suboptimal x-ray absorption at high x-ray tube potentials (120-140 kVp).…”

Section: Introductionmentioning

confidence: 99%

Optimization of the differentiation and quantification of high‐Z nanoparticles incorporated in medical devices for CT‐guided interventions

et al. 2020

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Purpose Material differentiation has been made possible using dual‐energy computed tomography (DECT), in which the unique, energy‐dependent attenuating characteristics of materials can provide new diagnostic information. One promising application is the clinical integration of biodegradable polymers as temporary implantable medical devices impregnated with high‐atomic number (high‐Z) materials. The purpose of this study was to explore the incorporation of high atomic number (high‐Z) contrast materials in a bioresorbable inferior vena cava filter for advanced CT‐based monitoring of its location and differentiating from surrounding materials. Materials and methods Imaging optimization and calibration studies were performed using a body phantom. The dual‐energy CT (DECT) ratios for iron, zirconium, barium, gadolinium, ytterbium, tantalum, tungsten, gold, and bismuth were generated for peak kilovoltage combinations of 80/150Sn, 90/150Sn, and 100/150Sn kVp in dual‐source CT via linear regression of the CT numbers at low and high energies. A secondary calibration of the material map to the nominal material concentration was generated to correct for use of materials other than iodine. CT number was calibrated to the material concentration based on single‐energy CT (SECT) with additional filtration (150Sn kVp). These quantification methods were applied to monitoring of biodegradable inferior vena cava filters (IVCFs) made of braided poly(p‐dioxanone) sutures infused with ultrasmall bismuth nanoparticles (BiNPs) implanted in an adult domestic pig. Results Qualitative material differentiation was optimal for high‐Z (>73) contrast agents in DECT. However, quantification became nonlinear and inaccurate as the K‐edge of the material increased. Using the high‐energy (150Sn kVp) data component as a SECT scan, the linearity of quantification curves was maintained with lower limits of detection than with DECT. Among the materials tested, bismuth had optimal differentiation from iodine in DECT while maintaining increased contrast in high‐energy SECT for quantification (11.5% error). Coating the IVCF with BiNPs resulted in markedly greater radiopacity (maximum CT number, 2028 HU) than that of an uncoated IVCF (maximum CT number, 127 HU). Using DECT imaging and processing, the BiNP‐IVCF could be clearly differentiated from iodine contrast injected into the inferior vena cava of the pig. Conclusions These findings may improve widespread integration of medical devices incorporated with high‐Z materials into the clinic, where technical success, possible complications, and device integrity can be assessed intraoperatively and postoperatively via DECT imaging.

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Radiopaque scaffolds based on electrospun iodixanol/polycaprolactone fibrous composites

Cited by 6 publications

References 64 publications

Bioresorbable Mesenchymal Stem Cell–Loaded Electrospun Polymeric Scaffold Inhibits Neointimal Hyperplasia Following Arteriovenous Fistula Formation in a Rat Model of Chronic Kidney Disease

Bioresorbable Mesenchymal Stem Cell–Loaded Electrospun Polymeric Scaffold Inhibits Neointimal Hyperplasia Following Arteriovenous Fistula Formation in a Rat Model of Chronic Kidney Disease

A Facile Composite Strategy to Prepare a Biodegradable Polymer Based Radiopaque Raw Material for “Visualizable” Biomedical Implants

Optimization of the differentiation and quantification of high‐Z nanoparticles incorporated in medical devices for CT‐guided interventions

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