Radionuclide Uptake and Transport on Microbes in Potential Repository Drifts at Yucca Mountain, Nevada

Abstract

Radionuclide adsorption onto microbes, microbial retention in the engineered barrier system (EBS), and their potential release from the EBS as microbial colloids have been investigated. The microbial source term for these calculations was derived using MING V 1.0 software code [1]. Multiple model calculations from MING representing variations in possible microbial communities in the EBS were abstracted into two equations representing one meter segments of potential repository drift containing either commercial spent nuclear fuel (CSNF) or defense high level waste (HLW) packages. These two equations (Equations 1 and 2) represent the average cumulative microbial biomass generated in the EBS at any given time. A distribution for uranium uptake onto microbes (162.88 ± 133.05 mg U/gm dry cell) was applied to the microbial source term. The distribution was derived from the data set in Suzuki and Banfield [2] representing 45 different species of bacteria and fungus, covering uranium uptake at optimum pH values of 1 to 7. The mass of uranium sorbed onto the biomass was either sequestered in the EBS or transported as a microbial colloid based on a regression of data from Jewett et al. [3] representing microbial sorption onto air-water interfaces in unsaturated column experiments. Over one million years, it is estimated that EBS microbes may adsorb from 77 to 2302 kg of uranium [2302 kg U > 100% of the uranium available in a one meter segment of a CSNF waste package] per meter of waste package depending on the saturation of the invert and type of waste package. Over the same time, microbial colloids may transport from 8 to 1250 kg of adsorbed uranium per meter of waste package from the EBS.

Cyclical Patterns Affect Microbial Dynamics in the Water Basin of a Nuclear Research Reactor

“…Microbes can be present in the form of planktonic populations or biofilms, which can adhere to metal surfaces in SNFPs, potentially leading to microbiologically influenced corrosion (MIC; Zhang et al, 1999;Giacobone et al, 2011;Smart et al, 2014). Although this phenomenon has been investigated, together with the radionuclide bioaccumulation potential of some bacterial strains (Jolley, 2002;Tisakova et al, 2013), these studies mainly focused on cultivation-based approaches at a single point in time to identify and characterize the detected microorganisms. However, only a small percentage of environmental bacteria can be cultivated under laboratory conditions (Whitman et al, 1998).…”