“…To increase sensitivity and reduce background, several variables, including probe concentration, probe label, buffers, or substrate, can be optimized (1,2,4,5). In addition, data resolution, or the ability to clearly distinguish data, can also be affected by various factors, such as the type and thickness of the film's emulsion, the distance between the sample and emulsion, or the exposure and the development time (3)(68). Along with the required experimental optimization, film quality and handling before and during development of the silver halide emulsion can contribute to background (3,68).…”