Radiomutants of chrysanthemum (Dendranthema grandiflora Tzvelev) of the Lady group: RAPD analysis of the genetic diversity

Abstract

Random amplified polymorphic DNA (RAPD) markers were used to study the molecular characterization of 10 new radiomutants of chrysanthemum. The original cultivar ÔRichmondÕ differed in genetic distance from its Lady group mutants. The analysis of genetic similarity indices revealed low diversity within the radiomutants. The dendrogram obtained after cluster analysis separated the new cultivars as a group that differed from the original cultivar ÔRichmondÕ. The Lady group cultivars, derived from one original cultivar by radiomutation, could be distinguished from each other by using RAPD markers of only a single primer or sets of two or three primers. Polymerase chain reaction analysis proved the efficiency of the RAPD method for DNA fingerprinting of the original cultivar ÔRichmondÕ and its new radiomutants.

Effect of radiation on regeneration of Chinese narcissus and analysis of genetic variation with AFLP and RAPD markers

“…This corresponds to a genetic variation frequency of 8.33%. RAPD method has successfully been used to study genetic variability in radiomutants from chrysanthemum (Ruminska et al 2004;Kumar et al 2006). The tissue culture-induced DNA polymorphisms detected with RAPD has been reported in some crops: 0.05%, 0.63% and 3.57% in sugar beet, garlic and soybean respectively (Munthali et al 1996;Al-Zahim 1999;Gesteira et al 2002).…”

Genetic Diversity of Chrysanthemum Plants Derived via Somatic Embryogenesis Using Rapd Markers

“…The authors also investigated the genetic stability of those cultivars and lines applying flow cytometry, however, they did not show any changes in the ploidy of the test plant material. The reports by Lema-Rumińska et al [2004] point to a variation in the genetic level (from 0.50 to 0.93) using RAPD markers in mutants in the 'Lady' cultivar group, including control cultivars 'Lady Salmon' (LS0) and 'Lady Vitroflora' (LV0), which was similar as in the study of the plants derived from somatic embryos. Similarly Chattarjee et al [2006], investigating 10 original chrysanthemum cultivars and 11 mutants, found a variation at a similar genetic level in the range from 0.17 to 0.90 using RAPD analysis.…”

“…PCR reactions were performed twice for all plant materials in Eppendorf tubes in 25µl containing 1 µl of template DNA (20 ng/µl), 12.5 µl 2 × PCR MIX PLUS (A&A Biotechnology, Poland), 2.5 µl of each primer (1 µM) and ultrapure water. Amplification was performed in Bio-Rad Thermal Cycler C1000 Touch TM (Hercules, CA, USA) programmed according to Lema-Rumińska et al [2004]. PCR products were separated on 1.5% agarose gels stained with ethidium bromide in 1 × TBE buffer.…”

“…The latter, even the smallest change point, can be detected using molecular markers. RAPD markers have been still used for the study of genetic differentiation between cultivars [Wolff and Peters-Van Rijn 1993, Wolff et al 1995, Wolff 1996, Myśków et al 2001, Mukherjee et al 2013, or a group of the cultivars produced by mutagenesis [Lema-Rumińska et al 2004, Chattarjee et al 2006, Miňano et al 2009].…”

Application of RAPD markers for characterization of γ-ray-induced rose mutants and assessment of genetic diversity

“…Recently, 11 radiomutants from two chrysanthemum cultivars were characterized by RAPD (Kumar et al 2006). This technique has also been used to study genetic variability in radiomutants from the lady group of chrysanthemum (Ruminska et al 2004). Similarly, a mutated cherry was differentiated from its parental by 1 out of the 40 RAPD primers tested (Stockinger et al 1996).…”