Radiometric selective inhibition tests for differentiation of Mycobacterium tuberculosis, Mycobacterium bovis, and other mycobacteria

Gross, W.; Hawkins, Jean E.

doi:10.1128/jcm.21.4.565-568.1985

Cited by 23 publications

(10 citation statements)

References 7 publications

(2 reference statements)

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“…M. bovis isolates were cultured immediately upon receipt in 20 ml of Middlebrook 7H9 broth (Difco Laboratories) supplemented with oleic acid-albumin-dextrose-catalase (OADC) enrichment medium (Difco) for 3 weeks at 37ЊC. The M. bovis isolates were further characterized on the basis of susceptibility to 5 g of thiophene carboxylic hydrazide (Sigma Chemical Co.) per ml in Middlebrook 7H10 agarose medium supplemented with OADC (9). The cultures were harvested following heat killing at 80ЊC for 40 min.…”

Section: Methodsmentioning

confidence: 99%

Molecular epidemiology of Mycobacterium bovis in Texas and Mexico

et al. 1996

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Seventy-nine Mycobacterium bovis isolates recovered from Mexican and Texas cattle were categorized into 16 and 25 distinct types on the basis of IS6110 and direct-repeat fingerprint patterns, respectively. By using a combination of both fingerprint patterns, 30 distinct restriction fragment length polymorphism types were defined. Fifty-eight of 79 isolates (73%) were distributed among nine clusters. Clustered isolates were identified within herds, as well as in geographically disperse herds in Texas and Mexico. This observation is consistent with active transmission within herds and among herds, presumably as a result of active or historical cattle movements. The majority of bovine isolates (64 of 79) exhibited a single copy of IS6110. Interestingly, in contrast to previous studies, a high percentage of bovine isolates (15 of 79) exhibited multiple IS6110 copies (two to five) distributed among 11 different restriction fragment length polymorphism types. It is speculated that transmission from noncattle sources may be responsible. Continued fingerprinting of isolates originating from nonbovine sources and herd surveys is expected to provide useful information regarding the epidemiology of tuberculosis in this region.

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Section: Methodsmentioning

confidence: 99%

Molecular epidemiology of Mycobacterium bovis in Texas and Mexico

et al. 1996

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“…However, subculture and conventional biochemical testing are still necessary for identification of the M. avium complex with the BACTEC system. This can require an additional 2 to 4 weeks or more (11,14). Use of the DNA probe test can reduce the time for identification of the M. avium complex in BACTEC cultures to 1 week.…”

Section: Discussionmentioning

confidence: 99%

“…for mycobacterial cultures has greatly decreased the time for final identification and susceptibility testing of M. tuberculosis, with detection times of 2 to 39 days (14,16). M. tuberculosis isolates can be presumptively identified by comparing growth in a control vial with growth in a second vial containing p-nitro-a-acetylamino-phydroxypropiophenone (NAP) (11). Strains that are inhibited by NAP are presumed to be M. tuberculosis, whereas those that grow are presumed to be MOTT bacilli (11).…”

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confidence: 99%

Whole chromosomal DNA probes for rapid identification of Mycobacterium tuberculosis and Mycobacterium avium complex

Roberts¹,

McMillan²,

Coyle³

1987

J Clin Microbiol

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Whole chromosomal DNA probes were used to identify clinical isolates of Mycobacterium tuberculosis, Mycobacterium avium complex, and Mycobacterium gordonae. The probe for M. tuberculosis was prepared from Mycobacterium bovis BCG, which has been shown to be closely related to M. tuberculosis. A probe for the M. avium complex was prepared from three strains representing each of the three DNA homology groups in the M. avium complex. The probes were used in dot blot assays to identify clinical isolates of mycobacteria. The dot blot test correctly identified 57 of the 61 (93%) cultures grown on solid media, and 100% of antibiotic-treated broth-grown cells were correctly identified. Identification by dot blot required a maximum of 48 h. When the probes were tested against 63 positive BACTEC (Johnston Laboratories, Inc., Towson, Md.) cultures of clinical specimens, 59% were correctly identified. However, of the 14 BACTEC cultures that had been treated with antibiotics before being lysed, 13 (93%) were correctly identified.

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“…[4,5] Patients with M.tb infection are easily and successfully treated with primary antituberculosis drugs, while the therapy of the disease caused by NTM is often long, strenuous, and not always successful. [6] Thus, it is important to differentiate infections due to M.tb and NTM at the early stage of the disease.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Rapid Differentiation Test for Mycobacterium Tuberculosis from other Mycobacteria by Selective Inhibition with p-nitrobenzoic Acid using MGIT 960

Sharma

Malhotra

et al. 2010

J Lab Physicians

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Tuberculosis is caused by Mycobacterium tuberculosis (M.tb) as well as Non-tubercular mycobacterium (NTM) with similar clinical presentation. Infections due to NTM are reported to have increased in the past few years. Growth of M.tb is inhibited by p-Nitrobenzoic acid (PNB), whereas, NTM are resistant. One hundred and nine isolates from various clinical samples were identified up to species level by their growth rate, pigmentation, and a battery of biochemical tests, including niacin accumulation, nitrate reduction, and heat-stable catalase (68°C) reactions. Para-nitrobenzoic acid (PNB) inhibition test was performed to differentiate between M.tb and NTM. PNB was added to the Lowenstein-Jensen (LJ) medium and BACTEC™ MIGIT (Mycobacteria Growth Indicator Tube)960 medium to a final concentration of 500 μg/ml. All the M.tb isolates, including Mycobacterium tuberculosis H37Rv (standard strain), were inhibited by PNB on both LJ and MGIT 960. Of the NTM isolates, all were resistant to PNB on MGIT 960 and on LJ PNB, except one isolate of Mycobacterium marinum that was resistant to MGIT 960 PNB, but was susceptible to LJ PNB. The reporting time for M.tb ranged from 4–11 days (median 5.9 days) by MGIT 960 and for NTM it was 2–10 days with an average of 4.5 days. This study was carried out to establish the accuracy and efficiency of MGIT 960 PNB and to differentiate between M.tb and NTM.

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Radiometric selective inhibition tests for differentiation of Mycobacterium tuberculosis, Mycobacterium bovis, and other mycobacteria

Cited by 23 publications

References 7 publications

Molecular epidemiology of Mycobacterium bovis in Texas and Mexico

Molecular epidemiology of Mycobacterium bovis in Texas and Mexico

Whole chromosomal DNA probes for rapid identification of Mycobacterium tuberculosis and Mycobacterium avium complex

Evaluation of a Rapid Differentiation Test for Mycobacterium Tuberculosis from other Mycobacteria by Selective Inhibition with p-nitrobenzoic Acid using MGIT 960

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