Radiometric Method for Detection of Bacteremia

Washington, John A.; Yu, Pauline K. W.

doi:10.1128/aem.22.1.100-101.1971

Cited by 14 publications

(12 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This period is shorter for gram-negative organisms than for streptococci; with inocula as low as 10 organisms this may take up to 15 h (7), by which time most organisms will grow to 107colony-forming units per ml. Our experiments clearly show a lag between growth of inocula in aerobic vials and their radiometric detection, corroborating the findings of Washington and Yu (6).…”

Section: Discussionsupporting

confidence: 91%

False no-growth blood cultures in pneumococcal pneumonia

Adeniyi-Jones¹,

Stevens²,

Rasquinha³

1980

J Clin Microbiol

View full text Add to dashboard Cite

The growth of Streptococcuspneumoniae in commercial media containing`4Clabeled substrates was studied experimentally; the results of blood cultures that were positive for S. pneumoniae over a 14-month period were analyzed to explain no-growth but radiometrically positive blood cultures from four patients with clinically diagnosed pneumococcal pneumonia. The growth of S. pneumoniae in aerobic blood culture vials resulted in a chocolate color in the medium. S. pneumoniae grew rapidly in both aerobic and anaerobic media, but 14CO2 evolved from the metabolism of the labeled substrates was detected only in the aerobic culture vials. Radiometric detection lagged behind growth of the organisms and was accompanied by visual changes in the media. By 24 h, the viability of the culture was on the decline; radiometric readings remained positive even when the culture had died. The isolation of bacteria from the blood has major clinical import. The earlier this happens, the better, so that appropriate therapy can be instituted. The initial detection of bacteremia

show abstract

Section: Discussionsupporting

confidence: 91%

False no-growth blood cultures in pneumococcal pneumonia

Adeniyi-Jones¹,

Stevens²,

Rasquinha³

1980

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…Some investigators failed to detect some bacterial species using a radiometric technique (10). They indicated that their use of a rotary shaker rather than a magnetic stirrer to agitate cultures during incubation probably was not responsible for differences between their results and those of De Land and Wagner (3,4).…”

Section: Resultsmentioning

confidence: 96%

Radiometric Detection of Some Food-Borne Bacteria

Previte

1972

Applied Microbiology

View full text Add to dashboard Cite

Studies on detection of bacteria by radiometric techniques have been concerned primarily with aerobic species in clinical specimens. The data presented here are related to detection of aerobic and anaerobic species that are of significance in foods, by measurement of "4CO2 evolved from the metabolism of 14Cglucose. Salmonella typhimurium and Staphylococcus aureus were inoculated into tryptic soy broth containing 0.0139 ,gCi of 14C glucose/ml of medium. Detection times ranged from 10 to 3 hr for inocula of 100 to 104 cells/ml of broth. Heat-shocked spores of Clostridium sporogenes or C. botulinum were incubated in tryptic soy broth supplemented with Thiotone and NaHCO,. The medium was rendered anaerobic with N2. Spores were detected when 0.0833 M1Ci of labeled glucose was available/ml of medium but not when 0.0139 1Ci of glucose was present/ml. The spores required 3 to 4 hr longer for detection than did comparable numbers of aerobic vegetative cells. The results demonstrate the importance of availability of sufficient label in the media and the potential of the application of this technique for sterility testing of foods.

show abstract

“…Previous studies comparing the efficiency of radiometric detection of bacteremia and conventional techniques have been performed with two separate systems (6,7). In these studies, the blood was distributed between a BACTEC vial for radiometric detection and one or more bottles of broth which were observed for turbidity and subcultured.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the BACTEC System with Blind Subculture for the Detection of Bacteremia

Caslow

Ellner

Kiehn

1974

Applied Microbiology

View full text Add to dashboard Cite

One thousand blood specimens were cultured in BACTEC vials containing modified Columbia broth in aerobic, anaerobic, and hypertonic formulations. Radiometric readings and subcultures were performed on aerobic and hypertonic vials at 24 h and 7 days, and on anaerobic vials at 48 h and 7 days. Significant numbers of false-positive BACTEC readings were obtained. Although all positive cultures were eventually detected by the BACTEC, approximately 20% of blood specimens yielding positive subcultures at 24 h did not give positive BACTEC readings until 48 h.

show abstract

Radiometric Method for Detection of Bacteremia

Cited by 14 publications

References 1 publication

False no-growth blood cultures in pneumococcal pneumonia

False no-growth blood cultures in pneumococcal pneumonia

Radiometric Detection of Some Food-Borne Bacteria

Comparison of the BACTEC System with Blind Subculture for the Detection of Bacteremia

Contact Info

Product

Resources

About