Radiolytic Synthesis of Colloidal Silver Nanoparticles for Antibacterial Wound Dressings

Abstract

Radiolytic synthesis provides a convenient and environmentally-friendly approach to prepare metallic nanoparticles in large scale with narrow size distribution. In this report, colloidal silver nanoparticles (AgNPs) were synthesized by gamma radiation using poly(vinyl alcohol) (PVA) or silk fibroin (SF) as stabilizers and were evaluated for their antibacterial properties. The conversion of metallic silver ions to silver atoms depended on irradiation dose and stabilizer concentration as determined by UV-Vis spectrophotometry and transmission electron microscopy. The uniformly dispersed AgNPs with diameter 32.3 ± 4.40 nm were evaluated as antiseptic agents in films composed of chitosan, SF, and PVA that were processed by irradiation-induced crosslinking. Using disc diffusion assay, the films containing 432 ppm AgNPs could effectively inhibit the growth of bothStaphylococcus aureusandPseudomonas aeruginosa. Therefore, we have demonstrated in our present study that gamma radiation technique can potentially be applied in the mass production of antibacterial wound dressings.

Certain Aspects of Silver and Silver Nanoparticles in Wound Care: A Minireview

“…Regarding its therapeutic properties silver is known for more than 1000 years BC. Water was stored in silver or copper vessels and exposed to light or filtered, unfit for human consumption [6,9,10]. Hippocrates-father of modern medicine-also used silver; he believed that silver powder has beneficial therapeutic effect and antidisease properties.…”

Biocidal properties study of silver nanoparticles used for application in green housing

“…During the last decades, silver metal nanoparticles (MNPs) have spurred a lot of interest due to their conductive, optical [1], catalytic [2], and more particularly biocidal properties [3,4]. In fact, among noble metals, Ag MNPs possess the most efficient biocidal properties with a very broad bactericidal and fungicidal activity spectrum.…”

Role of hydroxyl radical scavenger agents in preparing silver nanoparticles under γ-irradiation

“…IPA (or formic acid) plays an important role in scavenging the free radicals and are converted into organic radicals (refer to Eqs. 4,5,7,8,10). In this process, Ag + was successfully reduced to form Ag nanoparticles according to reactions proposed following Eqs.…”

“…A preparation method is than required to avoid the particles agglomeration and then to stabilize silver nanoparticles with non-toxic surfactants. [5][6][7][8].…”

“…The radiolytic synthesis method offers some profitability over the conventional methods because it can be carried out at ambient pressure and room temperature with high reproducibility and it provides fully reduced and highly pure nanoparticles free from by-products or chemical reducing agents. The method is able of controlling the particle size and structure which is influenced by certain parameters such as the choice of solvents and stabilizer, the precursor to stabilizer ratio, pH during synthesis, and absorbed dose [4,5,14,15].…”