Radiolytic Degradation of Uranyl-Loaded Tributyl Phosphate by High and Low LET Radiation

Ngelale, Randy; Lee, Christopher; Bustillos, Steven; Nilsson, Mikael

doi:10.1080/07366299.2018.1552549

Cited by 11 publications

(2 citation statements)

References 12 publications

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“…A metal sample holder was used to maintain consistent sample distance from the source. The dose rate has been previously calibrated with Fricke dosimetry. , Fricke dosimetry was used to confirm the expected dose for our sample position, volume, and vials . Solutions of 0.4 M sulfuric acid, 6 mM ammonium ferrous sulfate, and 1 mM potassium chloride were well-agitated for aeration and irradiated for 5 and 10 min.…”

Section: Methodsmentioning

confidence: 99%

Human γS-Crystallin Resists Unfolding Despite Extensive Chemical Modification from Exposure to Ionizing Radiation

et al. 2022

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Ionizing radiation has dramatic effects on living organisms, causing damage to proteins, DNA, and other cellular components. γ radiation produces reactive oxygen species (ROS) that damage biological macromolecules. Protein modification due to interactions with hydroxyl radical is one of the most common deleterious effects of radiation. The human eye lens is particularly vulnerable to the effects of ionizing radiation, as it is metabolically inactive and its proteins are not recycled after early development. Therefore, radiation damage accumulates and eventually can lead to cataract formation. Here we explore the impact of γ radiation on a long-lived structural protein. We exposed the human eye lens protein γS-crystallin (HγS) to high doses of γ radiation and investigated the chemical and structural effects. HγS accumulated many post-translational modifications (PTMs), appearing to gain significant oxidative damage. Biochemical assays suggested that cysteines were affected, with the concentration of free thiol reduced with increasing γ radiation exposure. SDS-PAGE analysis showed that irradiated samples form protein–protein cross-links, including nondisulfide covalent bonds. Tandem mass spectrometry on proteolytic digests of irradiated samples revealed that lysine, methionine, tryptophan, leucine, and cysteine were oxidized. Despite these chemical modifications, HγS remained folded past 10.8 kGy of γ irradiation as evidenced by circular dichroism and intrinsic tryptophan fluorescence spectroscopy.

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Section: Methodsmentioning

confidence: 99%

Human γS-Crystallin Resists Unfolding Despite Extensive Chemical Modification from Exposure to Ionizing Radiation

et al. 2022

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“…In addition, the TBP can be used as an organic solvent to extract minerals from rocks [1][2][3][4]. The most important use of TBP is in extracting the hexavalent uranium and the tetravalent plutonium, to form radioactive complexes in the processing of nuclear fuel by the PUREX process (Plutonium Uranium Refining by Extraction) [5][6][7][8][9]. Thus, it is not surprising that TBP and its derivatives have recently been documented as newly emerging pollutants of the environments and, therefore, may pose health hazards for humans and animals [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Spectral, Structural, Stability Characteristics and Frontier Molecular Orbitals of Tri-N-Butyl Phosphate (Tbp) and Its Degradation Products: DFT Calculations

HUSSEIN¹,

ELKHAIR²,

ELZUPIR³

et al. 2021

JOR

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This work demonstrates the facilities offered by density functional theory (DFT) to calculate the molecular structural and spectral properties of tri-n-butyl phosphate (TBP) and its derivatives, namely, di-n-butyl phosphate (HDBP) and mono-n-butyl phosphate (H2MBP). The computed parameters including frontier molecular orbitals and HOMOLUMO transitions indicated the TBP has the lowermost total energy with a lager HOMOLUMO energy bandgap followed by HDBP and H2MBP. These outcomes in terms of reactivity and stability were reversed counting their dipole moments. The simulated spectra of FTIR and Raman suggested these techniques could be considered as powerful for qualitative analysis of these compounds.

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Treatment of tributyl phosphate by fenton oxidation: Optimization of parameter, degradation kinetics and pathway

Wang

2023

Chemosphere

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Radiolytic Degradation of Uranyl-Loaded Tributyl Phosphate by High and Low LET Radiation

Cited by 11 publications

References 12 publications

Human γS-Crystallin Resists Unfolding Despite Extensive Chemical Modification from Exposure to Ionizing Radiation

Human γS-Crystallin Resists Unfolding Despite Extensive Chemical Modification from Exposure to Ionizing Radiation

Spectral, Structural, Stability Characteristics and Frontier Molecular Orbitals of Tri-N-Butyl Phosphate (Tbp) and Its Degradation Products: DFT Calculations

Treatment of tributyl phosphate by fenton oxidation: Optimization of parameter, degradation kinetics and pathway

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