Radiological and histological analysis of cortical allografts: an experimental study in sheep femora

Taira, Hirofumi; Moreno, José Luis López; Ripalda, P.; Forriol, Francisco

doi:10.1007/s00402-004-0653-x

Cited by 39 publications

(50 citation statements)

References 15 publications

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“…Results were evaluated according to the radiographic scoring system suggested by Taira et al (2004) (Table 1). For comparison of the radiographic score between two groups, Mann-Whitney U test was used.…”

Section: Radiologic Analysismentioning

confidence: 99%

Autologous mesenchymal stem cells loaded in Gelfoam® for structural bone allograft healing in rabbits

et al. 2010

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This study was designed to evaluate the effect of autologous bone marrow mesenchymal stem cells (MSCs) seeded into Gelfoam® on structural bone allograft healing. Thirty New Zealand white rabbits were divided into two groups. Segmental bone defect was created on diaphysis of the femur, and the defect was reconstructed with structural bone allograft. In experimental group, structural allograft was wrapped around by Gelfoam® containing autologous MSCs, whereas cells were not included in control group. At 4, 8, 12 weeks, the femur of rabbits underwent radiographic and histologic evaluation for bony union. Bone morphogenic protein-2 (BMP-2), BMP-4, BMP-7, vascular endothelial growth factor (VEGF), and receptor activator of nuclear factor-kappa B ligand (RANKL) were measured within the grafted periosteal tissue. Bony union was not achieved in both groups at 4 and 8 weeks. At 12 weeks, three out of five femurs in experimental group were united, but one out of five in control group was united. Mean Taira scores were significantly different between two groups. The expression of BMP-2 was significantly higher at 4, 8 weeks, the expressions of BMP-4 and BMP-7 were significantly higher at 8 and 12 weeks, and the expression of VEGF and RANKL were significantly higher at all time points in experimental group. Incorporation of the structural bone allograft could be enhanced if allograft is covered with Gelfoam® containing autologous MSCs. MSCs have influence on not only bone formation, but neo-angiogenesis, and bone resorption.

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Section: Radiologic Analysismentioning

confidence: 99%

Autologous mesenchymal stem cells loaded in Gelfoam® for structural bone allograft healing in rabbits

et al. 2010

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“…Autogenous bone grafts allow for revascularization and the migration of bone forming and resorbing cells onto and into tissue. 8,9 The most commonly used harvest site for autogenous grafts is the iliac crest. 10 Other sites include, but are not limited to, the proximal tibia, proximal humerus, fibula, and ribs.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Assessment of the Bone Morphogenetic Protein Expression From Alternate Bone Graft Harvesting Sites

Takemoto

Fajardo

Kirsch³

et al. 2010

Journal of Orthopaedic Trauma

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“…In order to get better graft incorporation, multi-perforations of the allograft bone have been advocated as a means to increase the interface between living soft tissues of the host and the allografted bone and to achieve a higher new bone formation [8,27]. However, perforation of the cortical allograft bone weakened the biomechanical properties and increased the risk of fracture.…”

Section: Discussionmentioning

confidence: 99%

“…Radiographs were graded independently by two observers according to a modified scoring system [27] (Table 1). Then the proximal femurs with allograft bone in situ were resected from the host bone, and contact radiographs were taken vertical to the longitudinal axis of the femur.…”

Section: Radiographymentioning

confidence: 99%

Effects of rhBMP-2 on cortical strut allograft healing to the femur in revision total hip arthroplasties: an experimental study

Xiong

2006

International Orthopaedics (SICO

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We have studied the effects of recombinant human bone morphogenetic protein-2 (rhBMP-2) on cortical strut allograft healing and remodelling in revision hip arthroplasty. Thirty adult New Zealand rabbits underwent bilateral onlay allograft strut procedures to the femur using wires. The left femur (experimental side) received the rhBMP-2 device (1.0-mg rhBMP-2/gelatin composites) interposed between the allograft and host bone, while the right side was grafted with an allograft strut as the control. The femurs and implants were retrieved at 4, 6, and 8 weeks postoperatively. The healing of cortical strut grafts to the femur was enhanced dramatically by the addition of the rhBMP-2 device in radiographic examination, contact radiographic examination, non-decalcification sections, fluorescence tag, and computer-aided image analysis. The remodelling of cortical strut allograft was also accelerated. The new bone formation ratio and radiographic scores of the experimental side were also much higher than the control side at all times. Strut healing with the rhBMP-2 device at 4 weeks postoperatively was superior to the healing in control sides at 8 weeks. Our findings showed that the rhBMP-2 device improved and accelerated the course of cortical strut allograft healing and remodelling with host bone.Résumé Nous avons étudié les effets de la BMP recombinante rhBMP-2 sur des allogreffes corticales dans les révisions de prothèses totales. 30 lapins adultes de Nouvelle Zélande ont bénéficié d'une allogreffe bilatérale sur un fémur cerclé avec interposition entre l'allogreffe et l'os receveur au niveau du fémur gauche de rhBMP-2 (1.0 mg rhBMP-2 de gélatines composites), le fémur droit étant greffé avec une allogreffe simple (groupe contrôle). Les fémurs et les implants ont été prélevés à 4, 6, 8 semaines post opératoires. La consolidation par allogreffe corticale a été considérablement améliorée par l'addition de rhBMP-2, tant sur le plan radiographique que sur le plan histologique. La consolidation avec rhBMP-2 à 4 semaines post opératoires est supérieure au groupe contrôle à 8 semaines. Notre expérimentation nous permet de montrer que le rhBMP-2 améliore la consolidation et le remodelage au niveau de l'os receveur des allogreffes corticales.

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Radiological and histological analysis of cortical allografts: an experimental study in sheep femora

Abstract: We believe that endochondral ossification is probably a biological event occurring routinely during the bone healing process and that the processes of incorporation of variously treated cortical allografts differ only at the early phase of implantation.

Cited by 39 publications

References 15 publications

Autologous mesenchymal stem cells loaded in Gelfoam® for structural bone allograft healing in rabbits

Autologous mesenchymal stem cells loaded in Gelfoam® for structural bone allograft healing in rabbits

Quantitative Assessment of the Bone Morphogenetic Protein Expression From Alternate Bone Graft Harvesting Sites

Effects of rhBMP-2 on cortical strut allograft healing to the femur in revision total hip arthroplasties: an experimental study

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