Radioiodination of balsalazide, bioevaluation, and characterization as a highly selective radiotracer for imaging of ulcerative colitis in mice

Abstract

This work focuses on tracking ulcerative colitis in mice. High labeling yield and radiochemical purity were achieved for the formation of a [125/131I]balsalazide radiotracer at optimum conditions of oxidizing agent content (chloramines‐T [Ch‐T], 75 μg), substrate amount (100 μg), pH of reaction mixture (6), reaction time (30 min), and temperature (37°C), using radioactive iodine‐125 (200–450 MBq). The radiolabeled compound, [125/131I]balsalazide, was stable in serum and saline solution during 24 h. Balsalazide is acting as a peroxisome proliferator‐activated receptor (PPARγ). Biodistribution studies were carried in normal and ulcerated colon mice. High uptake of 75 ± 1.90% injected dose/g organ (ID/g) observed in ulcerated mice confirmed the suitability of [131I]balsalazide as a novel radiotracer for ulcerative colitis imaging in mice.

Radioiodination of zearalenone and determination of Lactobacillus plantarum effect of on zearalenone organ distribution: In silico study and preclinical evaluation

“…The total yield of the radiotracer consists of free [ 125 I] -iodide and [ 125 I] -ZEN it is calculated from the percentage (%), on TLC at RTs (retention times) 0.0 and 0.9–1.0 respectively. HPLC analysis revealed the following, the purity for [ 125 I]-ZEN reached 99 % [ [101] , [102] , [103] , [104] , [105] , [106] , [107] , [108] , [109] , [110] , [111] , [112] , [113] , [114] , [115] , [116] , [117] , [118] , [119] , [120] ]. The Retention times values for both the free iodide and the labeled molecule [ 125 I]-ZEN existed at 4.5 and 15.8 min, respectively, with regard to the retention time for ZEN that was 14.7 min ( Fig.…”

Facile one-pot strategy for radiopreprationof radioiodinated phenylpiracetam as a new highly selective radiotracer for brainimaging

“…A standard deviation (SD) for the mean value of the percentage of the supplied dose per gram was determined. It was calculated that muscle made up 40% of a person's body weight, whereas bone made up 10%, and blood made up 7% [71][72][73][74][75][76][77][78][79][80]. Experiments were conducted while corrections were used for background radiation and emission decays.…”

Radioiodination, and Biological Assessment of Olsalazine, as a Highly Selective Radiotracer for Ulcerative Colitis Imaging in Mice

“…2B). By studying the effect of pH in the range 2-15, it was discovered that pH 8 is the optimum value for achieving the highest labelling yield of [ 125 I] iodoolsalazine radiotracer [84]. Furthermore, the effect of the reaction time and oxidizing agent (Ch-T) amount was investigated to obtain the highest labelling yield (98.5%) at 30 minutes and 100 μg, respectively (Fig.…”

“…XX, X, (2023) twenty-four hours post-injection. So, we look forward to finding a new radiotracer compound that is localized in the target organ with a higher percentage than the compounds that were studied, up to 24 h. [83,84].…”