Radioimmunological Determination of Human C-Peptide in Serum

Heding, L. G.

doi:10.1007/978-94-011-7094-9_10

Cited by 163 publications

(174 citation statements)

References 11 publications

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“…Plasma glucose was measured enzymatically, whereas plasma insulin was measured by RIA with polyethylene glycol separation [25,26]. Plasma C-peptide levels were measured by a modification of the method of Heding [27], using polyclonal antibody A-4741 (Ventrex, Portland, ME, USA) and polyethylene glycol precipitation [26] as previously described [28]. The interassay CV was 1.3% for a basal glucose value set at 5.0 mmol/l.…”

Section: Methodsmentioning

confidence: 99%

High normal 2-hour plasma glucose is associated with insulin sensitivity and secretion that may predispose to type 2 diabetes

et al. 2005

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Section: Methodsmentioning

confidence: 99%

High normal 2-hour plasma glucose is associated with insulin sensitivity and secretion that may predispose to type 2 diabetes

et al. 2005

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“…Plasma insulin (immunoreactive insulin) was measured (in duplicate) by a modified double-antibody radioimmunoassay according to Hales and Randle [17] with a filtration instead of centrifugation [intra-assay coefficient of variation (CV) = 5.5%; inter-assay CV = 8.7%]. Plasma C-peptide was measured (in duplicate) by a radioimmunoassay according to Heding [18] (intra-assay CV = 3.1%; inter-assay CV = 10.8%). Plasma free fatty acids were measured by an enzymatic method [19] (intra-assay CV = 3.1%; inter-assay CV = 3.4%).…”

Section: Methodsmentioning

confidence: 99%

A randomized study over 13 cycles to assess the influence of oral contraceptives containing ethinylestradiol combined with drospirenone or desogestrel on carbohydrate metabolism

Gaspard¹,

Scheen²,

Endrikat³

et al. 2003

Contraception

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In this open-label, randomized study we compared the influence of a new oral contraceptive containing 30 µg ethinylestradiol and 3 mg drospirenone (Yasmin™) with a reference preparation containing 30 µg ethinylestradiol and 150 µg desogestrel (Marvelon™) on variables of carbohydrate metabolism by means of oral glucose tolerance tests at baseline and in the 6th and 13th treatment cycle. The mean levels of fasting glucose and insulin were similar at baseline and after 13 treatment cycles, whereas C-peptide and free fatty acid levels decreased slightly in both groups. All blood glucose and insulin values measured in the oral glucose tolerance tests were within normal ranges, despite a slight increase in the mean areas under the curves of 0-3 h [AUCs (0-3 h)] of both variables from baseline to treatment cycle 13. Differences between both treatments were not statistically significant. The mean AUCs (0-3 h) for C-peptide were not markedly changed in any treatment group. Free fatty acid levels decreased by 42% in the drospirenone group and increased by 48.9% in the desogestrel group, in terms of means of individual changes. Both preparations were well tolerated and equally efficacious regarding contraception and cycle control. The mean body weight was slightly decreased in most cycles during treatment with the drospirenone combination, as compared to baseline, while it was slightly increased versus baseline in all cycles during treatment with the desogestrel combination. The combination with drospirenone had less impact on blood pressure than the combination with desogestrel. In conclusion, Yasmin, a combined low-dose oral contraceptive with 30 µg ethinylestradiol and 3 mg of the novel progestogen drospirenone, as well as the reference Marvelon, containing 30 µg ethinylestradiol and 150 µg desogestrel had little impact on carbohydrate metabolism when used for 1 year. The observed changes were small and not suggestive of a clinically relevant deterioration of carbohydrate metabolism.

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“…Serum insulin was measured using a double antibody technique [14]; the intra-and inter-assay coefficients of variation were 6.8 % and 7.9 %, respectively. Serum C-peptide was measured by ethanol precipitation radioimmunoassay [15] using the M1221 antibody and a synthetic standard (Novo Research Institute, B agsvaerd, Denmark).…”

Section: Assaysmentioning

confidence: 99%

Insulin sensitivity, insulin secretion and glucose effectiveness in diabetic and non-diabetic cirrhotic patients

et al. 1993

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Radioimmunological Determination of Human C-Peptide in Serum

Cited by 163 publications

References 11 publications

High normal 2-hour plasma glucose is associated with insulin sensitivity and secretion that may predispose to type 2 diabetes

High normal 2-hour plasma glucose is associated with insulin sensitivity and secretion that may predispose to type 2 diabetes

A randomized study over 13 cycles to assess the influence of oral contraceptives containing ethinylestradiol combined with drospirenone or desogestrel on carbohydrate metabolism

Insulin sensitivity, insulin secretion and glucose effectiveness in diabetic and non-diabetic cirrhotic patients

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