Radioimmunoassays for the Determination of the Indole Alkaloids Ajmalicine and Serpentine in Plants¹

Abstract

A Radioimmunoassay was developed for the individual measurement of ajmalicine and serpentine in plant extracts. Each RIA was assessed for its sensitivity, specificity and accuracy. The preparation of antigens and antibodies specific for these alkaloids as well' as the radiochemical preparation of ajmalicine-W and serpentine-3H with high specific activities is described.The RIA method allowed the detection of 0.27 pmol (0.1 ng) ajmalicine and 1.4 pmol (0.5 ng) serpentine and has been successfully applied in the selection of individual plants with higher than average contents of these alkaloids for breeding and tissue culture purposes.

Plant Cell Biotechnology

“…In order to attain a previously unachieved resolution, immunological testing methods were developed in addition to the classic physical methods (Fig. 30) of gas chromatography (GC) and high performance liquid chromatography (HPLC) ( et al 1978) no elaborate sample preparation is required (Arens et al 1978;Lehtola et al 1982).…”

Instability of Indole Alkaloid Production inCatharanthus roseusCell Suspension Cultures

“…Radioimmunoassay were used for the determination of serpentine and ajmalicine (12), as well as secologanin (13).…”

Radioimmuntests für die Bestimmung der Peptidealkaloide Ergotamin und Ergocristin in Claviceps purpurea

“…Die Durchfuhrung und Automatisierung der Radioimmuntests erfolgte wie in [9] beschrieben. Die optimalen Bedingungen fur die Antigen-und Antikorperreaktion und die Trennung der freien Antigene von den Antigen-Antikorperkomplexen waren fiir beide Tests iden-…”