Radioimmunoassay of steroids produced by cultured chick embryonic gonads: Differences according to age, sex, and side

Guichard, A.; Cédard, L.; Mignot, Thérèse-Marie; Scheib, D; Haffen, K

doi:10.1016/0016-6480(77)90204-0

Cited by 67 publications

(17 citation statements)

References 9 publications

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“…The present observation that transcripts for all the five genes required for the synthesis of estrogen are present in the regressing right gonad in female embryos is consistent with earlier studies in which small amounts of estrone and estradiol-17 were detected by radioimmunoassay in the cultured right gonad from female chicken or quail embryos (Guichard et al 1977, Teng & Teng 1977, Scheib et al 1981 or by immunofluorescence staining of gonadal sections from female chicken embryos (Woods & Erton 1978).…”

Section: Differential Gene Expression In Developing Chicken Gonads · supporting

confidence: 92%

“…The virtual absence of aromatase gene transcripts in the gonads of male embryos is consistent with the observation by Imataka et al (1989) that the production of radiolabeled estradiol-17 could be detected only in tissue slices of female chicken embryonic gonad incubated with a radiolabeled precursor steroid, but is inconsistent with the detection of small amounts of estrogen in male chicken embryonic gonads by radioimmunoassay or immunofluorescence staining (Guichard et al 1977, Woods & Erton 1978, Tanabe et al 1986). The latter observations may be explained by the detection of small amounts of aromatase cDNA fragment in male embryonic gonads after 40 cycles of PCR (Fig.…”

Section: Differential Gene Expression In Developing Chicken Gonads · supporting

confidence: 80%

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Differential expression of genes for aromatase and estrogen receptor during the gonadal development in chicken embryos

Nakabayashi¹,

Kikuchi²,

Kikuchi³

et al. 1998

Journal of Molecular Endocrinology

129

107

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In birds, differentiation of embryonic gonads is not as strictly determined by the genetic sex as it is in mammals, and can be influenced by early manipulation with a sex steroid hormone. Thus administration of an aromatase inhibitor induces testis development in the genetic female, and administration of estrogen induces a left ovotestis in the genetic male embryo. Another feature of avian gonadogenesis is that only the left ovary develops in most species. Molecular mechanisms underlying these features at the level of gene expression have not been elucidated. In this paper, we present evidence that a gene for aromatase cytochrome P-450, an enzyme required for the last step in the synthesis of estradiol-17 , is expressed in medullae of the left and right gonads of a female chicken embryo, but not in those of a male chicken embryo, and that an estrogen receptor gene is expressed only in epithelium (and cortex later, in the female) of the left, not the right, gonad of both sexes, but the expression in the male left gonad is temporary and restricted to an early stage of development. Differential expression of these two genes serves well to explain the above features of gonadal development in birds. Furthermore, in ovo administration of estradiol-17 from the 5th to the 14th day of incubation does not cause expression of the estrogen receptor gene in the right gonad of chicken embryos of either sex, suggesting that the absence of expression of the estrogen receptor gene in the right gonad is not the result of down-regulation, but may be regarded as an important cause of the unilateral ovarian development.

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Section: Differential Gene Expression In Developing Chicken Gonads · supporting

confidence: 92%

Section: Differential Gene Expression In Developing Chicken Gonads · supporting

confidence: 80%

Differential expression of genes for aromatase and estrogen receptor during the gonadal development in chicken embryos

Nakabayashi¹,

Kikuchi²,

Kikuchi³

et al. 1998

Journal of Molecular Endocrinology

129

107

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“…The present study indicates that duck embryonic and postembryonic ovaries are highly active in the production and secretion of testosterone and estradiol as compared with embryonic and postembryonic testes. Similar observations have been made in the embryonic chickens (Guichard et al 1977, 1979, Tanabe et al 1979. This is in contrast with the case of mammalian embryo, in which embryonic testes are much more active than embryonic ovaries (Rigaudiere 1977, George et al 1978, Wilson 1978.…”

Section: Methodssupporting

confidence: 78%

“…Whereas in birds some works have been reported only in the chicken (Woods et al 1975, Woods & Brazzill 1981, Guichard et al 1977, 1979, Tanabe et al 1979. Recently, it was found that H-Y antigen plays a pivotal role in the primary (gonadal) sex determination of mammals, and its regulatory gene exsists on Y chromosome of XY male cells in mammals, but it exists in ZW female cells (possibly on W chromosome) in avian species (Wachtel et al 1975a b, Ohno 1979, Ohno & Matsunaga 1981, Muller et al 1979, Zaborski et al 1980).…”

Section: Introductionmentioning

confidence: 99%

Ontogenetic Aspect of Steroidogenesis by Gonads and Adrenals of Ducks and Its Role on Sex Differentiation

Tanabe

1982

Journal of the Yamashina Institute for Ornithology

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Plasma testosterone, estradiol, corticosterone and cortisol levels together with gonadal testosterone and estradiol concentrations were determined by radioimmunoassay in the embryonic and post-embryonic Domestic Duck, Anas platyrhynchos var. domestica. The plasma testosterone and estradiol concentrations were higher in the female than in the male embryo , whereas no sex-difference was observed either in plasma corticosterone or cortisol . Both the ovarian estradiol and testosterone concentrations in the embryo were much higher in the testicular concentrations. The plasma corticosterone or cortisol concentration reached a peak one day after hatching, and decreased thereafter in both sexes. These results indicate that the ovary of embryonic duck is more active in secreting the sex steroid hormones than the embryonic testes , and suggest that the original sex of the duck (probably of all avian species) should be male (ZZ) , and estrogenic hormones secreted from the embryonic ovary has an important role in sexual development (feminizing) of the female in the duck.

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“…Voilà quelle était la situation quand les méthodes radioimmunologiques commencèrent à être employées (Guichard et al, 1977(Guichard et al, , 1979a (Chouraqui et al, 1980 Zeis, 1973bZeis, , 1974 (Weniger et al, 1981b(Weniger et al, , 1983, ainsi que du diéthylstilboestrol (Samsel et al, 19821 (Akram et al, 1973 ;Akram et Weniger, 1974) …”

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Sécrétion de stéroïdes par les gonades embryonnaires d'Oiseaux

Weniger¹

1987

Reprod. Nutr. Dévelop.

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Radioimmunoassay of steroids produced by cultured chick embryonic gonads: Differences according to age, sex, and side

Cited by 67 publications

References 9 publications

Differential expression of genes for aromatase and estrogen receptor during the gonadal development in chicken embryos

Differential expression of genes for aromatase and estrogen receptor during the gonadal development in chicken embryos

Ontogenetic Aspect of Steroidogenesis by Gonads and Adrenals of Ducks and Its Role on Sex Differentiation

Sécrétion de stéroïdes par les gonades embryonnaires d'Oiseaux

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