Radioimmunoassay of Plasma—prolactin

Wood, Peter; Shahwan, M. M.

doi:10.1016/s0140-6736(75)90328-1

Cited by 5 publications

(5 citation statements)

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“…One subject (subject 6) had a MCR measured twice, with the repeat value (45 ml/min per m2) being very similar to the original value (48 ml/min per m2). It was also demonstrated in this subject that there was no difference in the MCR of PRL iodinated with chloramine T vs. that iodinated with lactoperoxidase (13 There was no significant difference between these two groups (P 0.7).…”

Section: Resultsmentioning

confidence: 53%

“…In three reports (25)(26)(27), basal levels of PRL in hyperthyroid patients were equal to or higher than control, but the differences were not statistically significant. In a fourth study (28), the mean basal PRL of 12+6.9 ng/ml was significantly higher than control values 4.3+4.9 ng/ml FIGURE 5 (A) Sephadex G-100 chromatograph (1.5 x 90-cm column) of a typical iodination of PRL by lactoperoxidase (13). 1-ml fractions were collected and counted.…”

Section: Resultsmentioning

confidence: 93%

“…Fig. 5A shows the elution profile of '25I-PRL after iodination with lactoperoxidase (13). Three protein peaks (I, II, and III) were evident.…”

Section: Resultsmentioning

confidence: 99%

“…The horinone was reconstituted in 0.01 M NH4HCO3 buffer, pH 8.2, and stored at -70°C. lodination with 1251 to a specific activity of 50-100 ,uCi/,g was carried out using a modified chloramine T method or the lactoperoxidase method (13 At equilibriumn, labeled PRL was iminunoprecipitated fioni 0.8 ml of sera or infusate by the double antibody techni(lue using a final rabbit anti-PRI antibody titer of 1:100. The imnmunoprecipitability of infusate ranged froml 45 to 83% depending on the hormonie used for iodination, the specific activity, and the age of the iodinated PRL.…”

Section: Dopamine Infusion Decreased Prl Pr From 270 To 66mentioning

confidence: 99%

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Metabolic clearance and production rates of prolactin in man.

et al. 1979

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A B S T R A C T Metabolic clearance rates (MCR) and production rates (PR) of prolactin (PRL) have been determined by the constant infusion to equilibrium technique in 11 normal subjects, 6 patients with hyperthyroidism, 4 patients with hypothyroidism, and 9 patients with hyperprolactinemia. PRL MCR was also determined in four patients during dopamine infusion. Mean PRL MCR was 46±1 ml/min per m2 in women and 44±3 ml/min per m2 in men, and was significantly correlated with body mass (r = 0.84, P < 0.001). In contrast with controls, PRL MCR was higher in hyperthyroidism (MCR = 52±8 ml/min per mi2, P < 0.05), was slightly lower in hypothyroidism (MCR = 38+10 ml/min per m2, P = NS), and was significantly correlated with serum thyroxine (r = 0.46, P < 0.02). PRL MCR was lower than controls in hyperprolactinemia (MCR = 40+5 mllmin per i2, P <0.01) and was inversely correlated with serum PRL (r = -0.72, P < 0.001). PRL MCR was not significantly changed by dopamine infusion.Mean PRL PR for women and men was 211±74 and 187±44 ,ug/d per m2, respectively (P = NS). In hyperthyroidism the PRL PR was elevated (PR = 335±68 ,ug/d per m2, P < 0.02), but in hypothyroidism the increase (PR = 233±159 ,tg/d per m2) was not significant. In hyperprolactinemia the PRL PR was extremely high (PR = 31,000±29,000 ,ig/d per m2). Dopamine infusion decreased PRL PR from 270 to 66,ug/d per m2 indicating that its effect was on pituitary PRL secretion and not PRL metabolism.To evaluate possible circulating PRL heterogeneity that might arise during infusion, gel filtration of infusate and serum obtained during the MCR procedure was performed. Labeled monomeric PRL (peak III, Kav (partition coefficient) = 0.4) was partially A preliminary account of this work was presented at the 61st Annual Meeting of the Endocrine Society, Anaheim, Calif., 13-15 June, 1979. Received for publication 29 January 1979 and in revised form 9 August 1979. converted to two larger forms (peaks I and II) in vivo. Peak I (Kav = 0) was 30-40% immunoprecipitable, although peak II (Kav = 0.2) was not immunoprecipitable. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of peak I resulted in .90% conversion to peak III and restoration of full immunoactivity. Thus, peak

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Section: Resultsmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 93%

“…Fig. 5A shows the elution profile of '25I-PRL after iodination with lactoperoxidase (13). Three protein peaks (I, II, and III) were evident.…”

Section: Resultsmentioning

confidence: 99%

Section: Dopamine Infusion Decreased Prl Pr From 270 To 66mentioning

confidence: 99%

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Metabolic clearance and production rates of prolactin in man.

et al. 1979

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“…TSH was measured as described by Wood et al [16], prolactin by the method of Sinha et al [17] as modified by us [18], GH by the Lepetit Human GH Kit using IRP 66/217 as standard, LH as described by Wilde et al [19] using LH (MRC 68/ 40) as standard, FSH using non-LH cross-reacting antiserum and FSH (MRC 69/104) as standard, triiodothyronine (T3) using an antiserum raised at the University of Surrey and thyroxine (T4) using the Radiochemical Centre Kit. Testosterone was assayed by a non-chromatographic RIA using an antiserum raised at the University of Surrey and having minimal cross-reactivity with other steroid hormones and metabolites.…”

Section: Assay Methodsmentioning

confidence: 99%

Differences in pituitary and testicular function between diabetic patients on insulin and oral anti-diabetic agents

et al. 1978

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Summary.Pituitary responsiveness to thyrotrophin releasing hormone (TRH) and luteinizing hormone releasing hormone (LHRH) was studied in thirty one male diabetics, of whom sixteen were insulin-dependent and fifteen on oral ant• agents. Ten age-matched controls were also studied. TRH and LHRH were simultaneously administered intravenously, each in a small dose of 10 ~tg followed two hours later by 190 ~tg and 90 ~tg respectively. Basal hormone levels were measured in a further group of thirty six patients (twelve on insulin, twelve on oral agents and twelve on dietary restrictions alone).Higher thyrotrophin (TSH) response was observed following the small dose of TRH in the patients treated with oral agents than in the control subjects. The response of prolactin was lower in patients treated with oral agents compared with those treated with insulin. There was no difference in plasma 73 and T 4 levels in the patients treated with insulin or oral agents. Significantly higher basal growth hormone (GH) levels were observed in the diabetics. The insulin-dependent group showed a more marked response of GH to TRH/LHRH. No response was observed in the controls. Plasma testosterone levels were significantly lower in the oral agent group (13.8 nmol/1) than in the insulin group (19.4 nmol/1), patients on dietary restrictions (18.4 nmol/1) and the control subjects (19.0 nmol/1). The LH response to the smaller dose of LHRH was impaired in patients on insulin and oral agents. There was a significant difference in FSH response between impotent and sexually normal patients.

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