Radioimmunoassay of Plasma Adrenocorticotrophin in Cushing's Syndrome

Croughs, R. J. M.; Tops, C. F.; Jong, Frank H. de

doi:10.1677/joe.0.0590439

Cited by 11 publications

(4 citation statements)

References 16 publications

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“…Sufficient pituitary response of ACTH to LVP in Cushing's disease and no response of ACTH to LVP in adrenal tumors were demonstrated. These findings were already reported in the previous literature (Ney et al, 1963;Graber et al, 1965;Demura et al, 1966;Demura et al, 1972;Croughs et al, 1973) In patients with totally adrenalectomized Cushing's disease, the infusion of 150mg hydrocortisone did not suppress plasma ACTH by 30 min, while the suppression was already observed by 30min in 3 patients with Addison's disease whose basal ACTH levels were also high. This finding suggests the abnormality in the feedback mechanism of ACTH secretion continuing after the clinically effective treatment.…”

Section: Discussionsupporting

confidence: 85%

The Pituitary ACTH, GH, LH, FSH, TSH and Prolactin Reserves in Patients with Cushing's Syndrome

Hashimoto

1975

Endocrinol Japon

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In order to know the pituitary reserves of ACTH, GH, LH, FSH, TSH and prolactin in patients with Cushing's syndrome, the responses of these hormones to hydrocortisone, lysine-8 vasopressin (LVP), insulin-induced hypoglycemia, luteinizing hormon-releasing hormone (LH-RH) and thyrotropin releasing hormone (TRH) were examined before and after treatment. Fourteen patients with Cushing's disease (adrenal hyperplasia), 3 patientswith adrenal adenoma and one patient with adrenal carcinoma were investigated. Before treatment, sufficient response of plasma ACTH to LVP was observed in patients with Cushing's disease, while no response was observed in 3 patients with adrenal adenoma. There was no significant difference in the responses of other pituitary hormones between the patients with Cushing's disease. and the patients with adrenal adenoma. The response of plasma GH to insulin-induced hypoglycemia was impaired in most these patients. The response of plasma TSH to TRH was impaired in 6 of 8 patients tested. The response of plasma LH and FSH to LH-RH were preserved in 6 and 5 of 8 patients, respectively. The response of plasma prolactin to TRH was normal in most patients tested. After treatment, the improvements of the impaired responses of GH, TSH, LH and FSH wereobserved. Therefore, the impaired reserve observed in these patients before treatment seemed to be due to the hypercortisolemia. If the difference of the suppressibility of these pituitary hormones by cortisol may be judged simply from our observation, the orderof the suppressibility is supposed to be ACTH, GH, TSH, LH and FSH, and then prolactin.

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Section: Discussionsupporting

confidence: 85%

The Pituitary ACTH, GH, LH, FSH, TSH and Prolactin Reserves in Patients with Cushing's Syndrome

Hashimoto

1975

Endocrinol Japon

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“…We checked the antiserum against similar fragments and obtained similar results (Fig. 1), with the exception that the slope of the percentage bound v. log-dose curve of a25-39 ACTH was much flatter than that found by Croughs et al (1973). The slopes of serial dilutions of rat pituitary extracts and incubation medium from flasks containing rat pituitaries (in-vitro assay for CR-activity; below) were similar to that of the standard (Fig.…”

Section: Assay Methodssupporting

confidence: 53%

“…The characteristics of the Wellcome anti-ACTH serum have been examined by Croughs, Tops & de Jong (1973). We checked the antiserum against similar fragments and obtained similar results (Fig.…”

Section: Assay Methodsmentioning

confidence: 76%

Hypothalamic Luteinizing Hormone Releasing Factor and Corticotrophin Releasing Activity in Relation to Pituitary and Plasma Hormone Levels in Male and Female Rats

Chiappa¹,

Fink²

1977

Journal of Endocrinology

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Hypothalamic corticotrophin releasing (CR) activity and LH-releasing factor (RF) content, and pituitary and plasma LH, FSH and ACTH were measured in adult male and female Wistar rats maintained under 14 h light per day. Hypothalamic LH-RF and pituitary and plasma hormones were estimated by radioimmunoassay while CR-activity was assessed by the amount of ACTH released from hemipituitaries in vitro. Two experiments were carried out on male animals. In the first, some of the animals were kept in a room, distant from the animal house, in which the lighting was reversed with respect to the external environment. In animals exposed to the reversed lighting regime, hypothalamic LH-RF content and pituitary gonadotrophin concentrations were significantly lower than the values in male rats kept in the animal house where they were in close proximity to female rats. In the second experiment, which was carried out on animals which had all been kept in the animal house, there was no significant differences between the LH-RF contents measured at 3-4 h intervals throughout the day. Pituitary LH and FSH contents, but not concentrations, were significantly increased at 12.00 h. There was little differences between the experiments in CR-activity, plasma ACTH concentrations and profiles of pituitary ACTH content and concentration. As expected there was a diurnal rhythm in plasma corticosterone concentrations (determined by competitive protein-binding assay) with the peak occurring between 15.00 and 18.00 h. The profiles of plasma and pituitary ACTH were similar to that of plasma corticosterone. Corticotrophin releasing activity dropped significantly between 12.00 and 16.00 h, but remained steady at the other times. In female rats there were no significant differences between hypothalamic LH-RF content throughout the 4-day cycle. During pro-oestrus the mean LH-RF content rose to teach a high level at 18.00 h at which time plasma LH concentration had risen sharply to a level consistent with the peak of the preovulatory surge. Plasma FSH concentration also rose significantly between 15.00 and 18.00 h of pro-oestrus. At metoestrus and dioestrus, plasma FSH levels were lower in the morning than in the evening. These results suggest that (1) there is no diurnal rhythm in hypothalamic LH-RF, (2) there may be a diurnal rhythm in pituitary gonadotrophin content in the male and in plasma FSH concentration on the days of metoestrus and dioestrus in the female, (3) if a surge of LH-RF does occur on the afternoon of pro-oestrus, the rate of LH-RF synthesis exceeds its release, and (4) the mechanism which regulates gonadotrophin secretion in the male may be affected by factors in the environment other than daylength. The results provide further evidence for the view that the diurnal rhythm of corticosterone secretion is under hypothalamo-hypophysial control.

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“…Blood levels of ACTH were measured, using an immunoassay method (Croughs, Tops & de Jong, 1973) in 40 patients during the last 2 weeks of pregnancy. Subjects were chosen who were free of serious medical disease and who had no apparent obstetrical disorder.…”

Section: P Physiological Society December 1974mentioning

confidence: 99%

Communications

1975

The Journal of Physiology

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In order to understand the overall process of oxygenation within the lung or gill, it is necessary to measure the reaction velocity between oxygen and the red blood cell in situ. For this purpose Mochizuki, Tazawa & Ono (1974) developed a microphotometric technique which is most conveniently applied to gas exchange surfaces such as the chorioallantoic surface of the chick and the secondary gill lamella of fish. Gas mixtures were introduced into a moist chamber containing the secondary lamellae. The time course of the reaction recorded (Fig. 1) therefore includes the properties of the resistance to gas transfer across the gill membrane, plasma layer, and red cell membranes in addition to the reaction velocity between the oxygen and haemoglobin within the corpuscle.The time course of the oxygenation and deoxygenation of both carp and eel red blood corpuscles clearly show that deoxygenation takes place much more slowly than does the oxygenation process. Both processes 82P

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Radioimmunoassay of Plasma Adrenocorticotrophin in Cushing's Syndrome

Cited by 11 publications

References 16 publications

The Pituitary ACTH, GH, LH, FSH, TSH and Prolactin Reserves in Patients with Cushing's Syndrome

The Pituitary ACTH, GH, LH, FSH, TSH and Prolactin Reserves in Patients with Cushing's Syndrome

Hypothalamic Luteinizing Hormone Releasing Factor and Corticotrophin Releasing Activity in Relation to Pituitary and Plasma Hormone Levels in Male and Female Rats

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