Radioimmunoassay of Parathyroid Hormone in Human Plasma and Tissue Using Commercially Available Antiserum and Modified Iodination

Tanaka, Masahiro; Abe, Kaoru; Adachi, Isamu; Miyakawa, Sumiko; Kumaoka, Soichi

doi:10.1507/endocrj1954.21.173

Cited by 13 publications

(4 citation statements)

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“…The accumulation of aluminium in patients with chronic renal failure may occur as a result of three factors, namely: (1) renal mass reduction may induce aluminium accumula tion, since the kidney is the main route for excretion of aluminium in humans [8,23]; (2) long-term ingestion of an aluminium-containing phosphate binder could be a factor for aluminium accumulation [1,32]; (3) dialysis itself might be an important cause of aluminium accumulation [15,22,30]. Previously, we reported that an aluminium-containing phosphate binder had little effect on serum aluminium concentration in undialyzed and dialyzed patients [34].…”

Section: Discussionmentioning

confidence: 99%

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Influence of Aluminium on the Effect of 1α (OH) D<sub>3</sub> on Renal Osteodystrophy

Shimada

Nakamura

Marumo

1983

Nephron

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The influence of aluminium in dialysate on the effects of 1α(OH)₃ on hemodialyzed hypocalcemic patients with end-stage renal failure, was studied during a 24- to 42-month period. 51 hypocalcemic patients were divided into two groups; group 1 consisted of 28 patients who were dialyzed using dialysate prepared from reverse osmosed water; the 23 patients in group 2 used dialysate prepared from softened water. Aluminium concentration in the dialysate used for group 1 was less than the detectable limit (10 µg/l) in twelve times determinations, while that for group 2 was 23.1 ± 9.2 µg/l (mean ± SD, n = 14). By the administration of 1α(OH)D₃, the serum concentration of calcium was increased, and that of iPTH and alkaline phosphatase activity was decreased in both groups. Subperiosteal resorption of the finger bone, evaluated by Jensen’s criteria, was significantly improved in group 1, while there was no improvement in group 2. Serum aluminium concentration in the patients of group 1 and group 2 were 46.6 ± 6.3 and 84.7 ± 13.9 µg/l, respectively, and the concentration of the latter was significantly higher than that of the former (p < 0.01). It was also shown that there is a positive correlation between the extent of subperiosteal resorption and the concentration of aluminium in serum. Serum aluminium concentration and bone aluminium content were increased according to the duration of hemodialysis in the patients who were dialyzed using dialysate from softened water, while there was no correlation between the duration of hemodialysis and serum aluminium concentration for the patients of group 1. After changing the supply of water for dialysate, prepared from softened water to reverse osmosed water, serum aluminium concentration significantly decreased. These results are consistent with, but do not prove, the hypothesis that aluminium plays a pathological role in renal osteodystrophy.

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Section: Discussionmentioning

confidence: 99%

“…Serum iPTH concentra tion was determined by a double antibody radioimmunoassay tech nique using antibovine anti-iPTH serum (Wellcome Co. Ltd., Eng land), as described by Tanaka et al [32], Bovine PTH (Wilson Lab., USA) was used as a standard material.…”

Section: Methodsmentioning

confidence: 99%

Influence of Aluminium on the Effect of 1α (OH) D<sub>3</sub> on Renal Osteodystrophy

Shimada

Nakamura

Marumo

1983

Nephron

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“…Plasma HDL-cholesterol was measured by the heparin-Mn++ precipitation method [13], Plasma lecithin-cholesterol acyltransferase (LCAT) activity was measured according to the method of Nagasaki and Akannma [14], its activity being expressed as units, i.e. free cholesterol umol/ml/h at 37 C. Parathyroid hormone (PTH), which is likely to suppress the lipo protein lipase activity and, on the other hand, to stimulate lipolysis and release of free fatty acids into the bloodstream, was measured by radioimmunoassay [15].…”

Section: Methodsmentioning

confidence: 99%

Effects of a Polyunsaturated Fatty Acid-Rich Diet on Serum Lipids in Patients with Chronic Renal Failure

Tsukamoto

Okubo

Yoneda

et al. 1982

Nephron

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An abnormal level of serum lipids may be one of the major risk factors for the development of atherosclerosis in patients with chronic renal failure (CRF). In the present study, the effect of polyunsaturated fatty acid-rich and low carbohydrate diet on serum lipids and HDL-cholesterol was studied in 6 nonnephrotic and nondialyzed patients with CRF on an isocaloric basis. Serum triglycerides decreased significantly from 2.08 ± 0.93 mmol/l (183 ± 82 mg/dl) to 1.49 ± 0.83 mmol/l (131 ± 73 mg/dl) by consumption of polyunsaturated diet (p < 0.01). The ‘HDL-cholesterol ratio’ increased significantly and LCAT decreased on a polyunsaturated diet. In conclusion, the consumption of a polyunsaturated fatty acid-rich diet tends to normalise increased serum triglycerides and lowered ‘HDL-cholesterol ratio’ in patients with CRF and may be effective for prevention of atherosclerotic vascular sequellae.

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“…Plasma PTH levels were determined by the radioimmunoassay as reported previously (Tanaka et al, 1974). Calcium (Ca) and magnesium (Mg) were determined by atomic absorption spectrophotometry and phosphate (P) by the method of Goldenberg and Fernandez (Goldenberg and Fernandez, 1966).…”

Section: Methodsmentioning

confidence: 99%

Phosphaturic Effect of I. V. Administered Calcitonin in Man

et al. 1974

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SynopsisPorcine calcitonin (CT) was administered intravenously to 4 patients whose kidney function and plasma Ca and P levels were normal. A significant increase in urinary P excretion with an increase in urinary cyclic AMP (cAMP) excretion was observed. Plasma P and cAMP levels which were determined simultaneously did not show any significant change. Furthermore, plasma PTH levels determined by the radioimmunoassay revealed that the increased PTH levels provoked by the CT infusion was not responsible for an increase in urinary P or cAMP excretion, since no increase in plasma PTH levels was found during the period when an increase in urinary P or cAMP excretion was observed. In addition, the CT infusion caused an increase in urinary excretion of Ca, Na and Mg as well as P.Based on these data, it can be concluded that CT administered intravenously acts directly on the kidney, resulting in an increase in urinary P excretion which could be mediated through cAMP in the kidney.It is well known that calcitonin (CT) administration causes phosphaturia, but there is a controversy about the mechanism. In the rat, CT has been shown to increase the renal excretion of P in both intact and thyroparathyroidectomized animals (Kenny et al., 1965;Milhaud et al., 1966;Robinson et al., 1966). However, in the dog this effect depended on the presence of the parathyroids (Pak et al., 1970). Ardaillou et al. reported that a patient with hypoparathyroidism responded to porcine CT with a significant phosphaturia (Ardaillou et al., 1968). Haas et al. infused porcine, human or salmon CT to 8 parients with untreated surgical hypoparathyroidism and documented that pharmacological doses of CT increased the renal clearance of P, Ca, Na and Mg independently of the parathyroids (Haas et al., 1972).In the present study, porcine CT was infused to 3 patients with advanced breast cancer and a patient with Turner's syndrome whose renal function or plasma Ca and P levels were normal. Plasma Levels of P, Ca, PTH and porcine CT as well as urinary excretion of P, Ca and creatinine were determined to clarify whether the phosphaturic effect of the intravenouly administered CT in man was due to a direct effect on the kidney or to the PTH secreted in response to the CT administration. In addition, urinary levels of Na, Mg and K were measured following the CT infusion to examine the CT effect on these electrolytes excretion from the kidney.Furthermore, urinary excretion of adenosine 3',5'-monophosphate (cAMP) as well as plasma levels of cAMP was determined following the CT administration in order to

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Radioimmunoassay of Parathyroid Hormone in Human Plasma and Tissue Using Commercially Available Antiserum and Modified Iodination

Cited by 13 publications

References 10 publications

Influence of Aluminium on the Effect of 1α (OH) D<sub>3</sub> on Renal Osteodystrophy

Influence of Aluminium on the Effect of 1α (OH) D<sub>3</sub> on Renal Osteodystrophy

Effects of a Polyunsaturated Fatty Acid-Rich Diet on Serum Lipids in Patients with Chronic Renal Failure

Phosphaturic Effect of I. V. Administered Calcitonin in Man

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