Radioimmunoassay of Oestrone and Oestradiol in Human Plasma

Emment, Yvonne; Collins, William P.; Sommerville, Ian F.

doi:10.1530/acta.0.0690567

Cited by 148 publications

(39 citation statements)

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“…The mean and range of peripheral plasma estradiol concentrations in normal adult males and females obtained by the present method were similar to those reported by other workers (Mikhail et al, 1970;Wu and Lundy, 1971;Abraham et al, 1972;Emment et al, 1972;Saez et al, 1972;Wright et al, 1973).…”

Section: Discussionsupporting

confidence: 92%

Plasma Estradiol Concentrations and Effect of HCG on Plasma Estradiol and Testosterone in Normal Subjects and Patients with Endocrine Disorders

Ishimaru

1975

Endocrinol Japon

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Section: Discussionsupporting

confidence: 92%

Plasma Estradiol Concentrations and Effect of HCG on Plasma Estradiol and Testosterone in Normal Subjects and Patients with Endocrine Disorders

Ishimaru

1975

Endocrinol Japon

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“…Free testosterone (non-protein-bound) was calculated as described by Bartsch (5). Serum estradiol was measured using a specific in-house RIA after extraction chromatography, as described by Emment and Collins (14).…”

Section: Methodsmentioning

confidence: 99%

Suppression of endogenous testosterone production attenuates the response to strength training: a randomized, placebo-controlled, and blinded intervention study

Kvorning

Andersen

Brixen

et al. 2006

American Journal of Physiology-Endocrinology and Metabolism

145

114

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Kvorning, Thue, Marianne Andersen, Kim Brixen, and Klavs Madsen. Suppression of endogenous testosterone production attenuates the response to strength training: a randomized, placebo-controlled, and blinded intervention study. Am J Physiol Endocrinol Metab 291: E1325-E1332, 2006. First published July 25, 2006 doi:10.1152/ajpendo.00143.2006.-We hypothesized that suppression of endogenous testosterone would inhibit the adaptations to strength training in otherwise healthy men. Twenty-two young men with minor experience with strength training participated in this randomized, placebo-controlled, double-blinded intervention study. The subjects were randomized to treatment with the GnRH analog goserelin (3.6 mg) or placebo (saline) subcutaneously every 4 wk for 12 wk. The strength training period of 8 wk, starting at week 4, included exercises for all major muscles [3-4 sets per exercise ϫ 6 -10 repetitions with corresponding 6-to 10-repetition maximum (RM) loads, 3/wk]. A strength test, blood sampling, and whole body DEXA scan were performed at weeks 4 and 12. Endogenous testosterone decreased significantly (P Ͻ 0.01) in the goserelin group from 22.6 Ϯ 5.5 (mean Ϯ SD) nmol/l to 2.0 Ϯ 0.5 (week 4) and 1.1 Ϯ 0.6 nmol/l (week 12), whereas it remained constant in the placebo group. The goserelin group showed no changes in isometric knee extension strength after training, whereas the placebo group increased from 240.2 Ϯ 41.3 to 264.1 Ϯ 35.3 Nm (P Ͻ 0.05 within and P ϭ 0.05 between groups). Lean mass of the legs increased 0.37 Ϯ 0.13 and 0.57 Ϯ 0.30 kg in the goserelin and placebo groups, respectively (P Ͻ 0.05 within and P ϭ 0.05 between groups). Body fat mass increased 1.4 Ϯ 1.0 kg and decreased 0.6 Ϯ 1.2 kg in the goserelin and placebo groups, respectively (P Ͻ 0.05 within and between groups). We conclude that endogenous testosterone is of paramount importance to the adaptation to strength training.

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“…Estrone and estrone sulfate was measured in serum by radioimmunoassay [15,16]. SHBG was measured in serum by immunofluorometric analysis [17].…”

Section: Laboratory Assaysmentioning

confidence: 99%

Associations of Body Composition Measures and Endogenous Sex-Steroid Hormones among Postmenopausal Women

Mellemkjær¹,

Christensen²,

Frederiksen³

et al. 2011

TOOBESJ

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Abstract:Several studies have shown a positive association between body mass index (BMI) and endogenous levels of female sex-steroid hormones, but relationship with the body fat mass and the fat free mass components of BMI has rarely been investigated. In this cross-sectional study, we included 265 women from the Danish "Diet, Cancer and Health" cohort who were postmenopausal and never users of hormone replacement therapy and who previously were included in a nested case-control study on breast cancer. Multiple log-linear regression analyses of the association between anthropometric measurements and serum levels of estrone, estrone sulfate and sex hormone binding globulin (SHBG) were performed. We found positive associations between all the anthropometric measurements included and estrone and estrone sulfate, whereas negative associations were found for SHBG. We confirmed previous findings of associations between BMI and serum levels of estrogen and SHBG among postmenopausal women. Associations were in the same direction for the two components of BMI, body fat mass index (BFMI) and fat free mass index (FFMI), and use of these more detailed measures did not give a better prediction of the hormone levels than use of BMI alone.

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Radioimmunoassay of Oestrone and Oestradiol in Human Plasma

Cited by 148 publications

References 0 publications

Plasma Estradiol Concentrations and Effect of HCG on Plasma Estradiol and Testosterone in Normal Subjects and Patients with Endocrine Disorders

Plasma Estradiol Concentrations and Effect of HCG on Plasma Estradiol and Testosterone in Normal Subjects and Patients with Endocrine Disorders

Suppression of endogenous testosterone production attenuates the response to strength training: a randomized, placebo-controlled, and blinded intervention study

Associations of Body Composition Measures and Endogenous Sex-Steroid Hormones among Postmenopausal Women

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