Radioimmunoassay of Arginine Vasotocin

Rosenbloom, A.A.; Fisher, Delbert A.

doi:10.1210/endo-95-6-1726

Cited by 72 publications

(24 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A V T also has bccn measured in the cerebral spinal fluid of adult mammals, including man (7,15,17). w e have recently reported RIA systems for measurement of AVP (24) and AVT, using a differential antiserum protocol (21). The present study reports results applying this nlcthod to qualititation of AVP and AVT in early gestational age human fetal pituitary glands and late gestational age ovinc fetal pituitary glands.…”

Section: Speculationmentioning

confidence: 86%

See 1 more Smart Citation

Fetal Neurohypophyseal Arginine Vasopressin and Arginine Vasotocin in Man and Sheep

Fisher

1977

Pediatr Res

View full text Add to dashboard Cite

show abstract

Section: Speculationmentioning

confidence: 86%

“…Synthetic AVP ancl AVT a e r c iodinated, using a modification of the method of Hunter ancl Greenwood (24). Assay procedures werc carried out as described earlier (21,23). and bourid ant1 free hormones were scparaterl exclusively by the double antibody nicthod (13).…”

Section: Speculationmentioning

confidence: 99%

Fetal Neurohypophyseal Arginine Vasopressin and Arginine Vasotocin in Man and Sheep

Fisher

1977

Pediatr Res

View full text Add to dashboard Cite

show abstract

“…Arginine vasotocin (AVT; [8-arginine]-oxytocin), the most ubiquitous neurohypophysial principle in submammalian vertebrates, has been reported in fetal pituitary and pineal glands and/or adult pineal glands of a variety of mammalian species (4)(5)(6)(7)(8)(9). However, the mammalian occurrence of AVT is still not universally accepted (10,11), and AVT has not been reported in mammalian circulation.…”

mentioning

confidence: 99%

Arginine vasotocin in ovine fetal blood, urine, and amniotic fluid.

Ervin¹,

Leake²,

Ross³

et al. 1985

J. Clin. Invest.

View full text Add to dashboard Cite

Arginine vasotocin (18-argininel-oxytocin) (AVT), the primary antidiuretic principle in submammalian vertebrates, has been reported to be present in mammalian pituitary and pineal glands. Although the most phyletically ubiquitous of the known neurohypophysial peptides, AVT is still not recognized as a mammalian hormone. We examined plasma, urine, and amniotic fluid from fetal lambs by radioimmunoassay (RIA) for evidence of AVT to assess the possibility of its being such a hormone. Measureable quantities of AVT-like immunoreactivity (irAVT) were observed in fetal plasma (2.4±0.2 pg/ml), urine (1.4±0.2 pg/ml), and amniotic fluid (1.9±0.2 pg/ml). Since the AVT antiserum shows minimal cross-reactivity with arginine vasopressin (AVP) and oxytocin (OT), measurements of AVP and OT concentrations in the same biological fluids also were conducted with specific antisera. The results suggest that the irAVT was not accountable on the basis of cross-reaction. To further verify the identity of the irAVT, a high pressure liquid chromatography system using RIA as a means of detection was developed. This system is sufficiently sensitive to allow the separation and quantitation of picogram quantities of the synthetic peptides AVT, AVP, and OT. In this system, the irAVT in fetal plasma, urine, and amniotic fluid appeared as a single peak coeluting with synthetic AVT. These results indicate that AVT is present in ovine fetal plasma and support the view that the fetus secretes this peptide. The physiological significance of circulating AVT remains to be defined.

show abstract

“…Generally, the two species had similar plasma AVT concentrations (9.4-164·pg·ml -1 ) and most excavated individuals had mean concentrations not significantly different from control individuals. Concentrations were also similar to that of hydrated Rana ridibunda and Bufo marinus (Nouwen and Kühn, 1983;Nouwen and Kühn, 1985;Konno et al, 2005) but higher than hydrated Rana catesbeiana (Rosenbloom and Fisher, 1974;Sawyer and Pang, 1975;Pang, 1977). One cocooned N. aquilonius was found to have much higher AVT concentrations than any other frog (394·pg·ml -1 ).…”

Section: Arginine Vasotocinmentioning

confidence: 50%

“…The radioimmunoassay for AVT was modified from Rosenbloom and Fisher (Rosenbloom and Fisher, 1974) by Rice (Rice, 1980) and is described in detail elsewhere (Rice, 1982). Briefly, [Arg 8 ]-Vasotocin (Auspep Pty Ltd, Parkville, Victoria, Australia) was radioactively labelled with 125 I (Amersham Biosciences, UK) using the chloramine-T method (Hunter and Greenwood, 1962).…”

Section: Arginine Vasotocinmentioning

confidence: 99%

Water balance of field-excavated aestivating Australian desert frogs, the cocoon-formingNeobatrachus aquiloniusand the non-cocooningNotaden nichollsi(Amphibia: Myobatrachidae)

Cartledge

Withers

McMaster

et al. 2006

Journal of Experimental Biology

View full text Add to dashboard Cite

, except for one cocooned N. aquilonius with a higher concentration of 394·pg·ml -1 . For both species, AVT showed no relationship with plasma osmolality over the lower range of plasma osmolalities but was appreciably increased at the highest osmolality recorded. This study provides the first evidence that cocoon formation following burrowing is not obligatory in species that are capable of doing so, but that cocoon formation occurs when soil water conditions are more desiccating than for non-cocooned frogs.

show abstract

Radioimmunoassay of Arginine Vasotocin

Cited by 72 publications

References 0 publications

Fetal Neurohypophyseal Arginine Vasopressin and Arginine Vasotocin in Man and Sheep

Fetal Neurohypophyseal Arginine Vasopressin and Arginine Vasotocin in Man and Sheep

Arginine vasotocin in ovine fetal blood, urine, and amniotic fluid.

Water balance of field-excavated aestivating Australian desert frogs, the cocoon-formingNeobatrachus aquiloniusand the non-cocooningNotaden nichollsi(Amphibia: Myobatrachidae)

Contact Info

Product

Resources

About