Radioimmunoassay Measurement of Prostaglandins E, A, and F in Human Plasma

Jaffe, Bernard M.; Behrman, Harold R.; Parker, Charles W.

doi:10.1172/jci107196

Cited by 469 publications

(98 citation statements)

References 44 publications

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“…PGEz was measured by radioimmunoassay using PGE2 antiserum, as described by Jaffe et a1 (18) and by a method outlined b y the supplier (Seragen, Boston, MA). Crossreactivity of the antiserum at 50% B/Bo (normalized percent bound) is 2.7% for PGF2,, 10.6% for PGA2, 100% for PGEI, and negligible for 6-keto-PGF1, (0.3%), PGD:!…”

Section: Methodsmentioning

confidence: 99%

Interleukin‐1β and interleukin‐1α stimulate the plasminogen activator activity and prostaglandin E₂ levels of human synovial cells

Leizer

Clarris

Ash

et al. 1987

Arthritis & Rheumatism

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Monocyte-macrophage polypeptides (monokines) cause synovial cells to increase the levels of putative mediators of destruction and inflammation. This interaction may account for some of the properties of rheumatoid pannus. We report here that samples of purified human interleukin-lfi (IL-lp) and recombinant IL-la stimulate both the plasminogen activator activity and prostaglandin Ez levels of human synovial fibroblast-like cells. The same holds true for purified pig IL-1 (catabolin) and recombinant murine IL-1. The elevation in plasminogen activator activity was inhibited by indomethacin, and this suggests that endogenous prostanoids are important in the IL-1-mediated stimulation of proteinase activity.

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Section: Methodsmentioning

confidence: 99%

Interleukin‐1β and interleukin‐1α stimulate the plasminogen activator activity and prostaglandin E₂ levels of human synovial cells

Leizer

Clarris

Ash

et al. 1987

Arthritis & Rheumatism

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“…The fractions were then dried and reconstituted with 0.2 M phosphate buffer (pH 7.0) containing 1% rabbit serum. Radioimmunoassays for PGE and PGF content were carried out in the manner previously described (19). Measurements were corrected for final recovery of added tritiated PG, which averaged 40%,…”

Section: Diafiltration Of Mediamentioning

confidence: 99%

Monocytes regulate osteoclast-activating factor production by releasing prostaglandins.

Yoneda¹,

Mundy²

1979

The Journal of Experimental Medicine

129

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Osteoclast-activating factor (OAF), a powerful stimulator of osteoclastic bone resorption, is released by peripheral blood mononuclear cells on exposure to phytohemagglutinin (PHA) or a specific antigen to which the leukocytes have been previously exposed. Both lymphocytes and monocytes are required in the leukocyte population for OAF release to occur. In this study we examined the relationship between the lymphocyte and monocyte in OAF production. Biological activity, as a result of OAF, was assessed by a bioassay based on the release of previously incorporated 45Ca from fetal rodent long bones in organ culture. We found that an enriched lymphocyte population depleted of monocytes by serial adherence does not release OAF after stimulation with PHA, although the cells are activated as assessed by [3H]thymidine and 3H-amino acid incorporation. When conditioned media harvested from adherent cells which did not contain OAF was added to the enriched lymphocytes, OAF release occurred. Media harvested from adherent cells which were cultured with indomethacin (10 microM), an inhibitor of prostaglandin synthesis, did not permit OAF release by activated lymphocytes. When PGE1 and PGE2 (0.1 microM) were added exogenously to the enriched lymphocyte population, OAF release occurred after stimulation with PHA. These results indicate that, (a) the activated lymphocyte is the cell or origin of OAF, (b) prostaglandins produced by monocytes are necessary for OAF production by activated lymphocytes, and (c) monocyte prostaglandins can influence bone resorption indirectly by regulating OAF production as well as directly by osteoclast activation. The interactions of OAF and prostaglandins at bone resorbing sites may be important in inflammatory and neoplastic diseases associated with bone destruction.

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“…The homologous PgE 2 Pl~ and anti-P~ anribodm in the same serum, bind. ,me of oar mtisera apmst PgA 2 and PIll [10,12] m s P~ and PIgB better than the anti-P~ antibodies raN/Shill [5] amtimra dhould provide a a~nnp4e do. might contribute to tim ~fkity of the PI~ amy.…”

Section: Discussionmentioning

confidence: 93%

“…In fact, several investigators [2,3] have suggested that it would be impossible to obtain sufficiently specific antibodies against PgE and PgA. Only recently the production of more specific antisera against PgE 1 [11,12] and PgA 1 [5] has been described. However, there is also much interest in the specific measurement of the dienoic prostaglandins PgE 2 PgA 2, since they seem to be widely distributed in biological material.…”

Section: Introductionmentioning

confidence: 99%

On the specificity of antisera against prostaglandins A₂ and E₂

Jobke

Peskar

Peskar³

1973

FEBS Letters

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Radioimmunoassay Measurement of Prostaglandins E, A, and F in Human Plasma

Cited by 469 publications

References 44 publications

Interleukin‐1β and interleukin‐1α stimulate the plasminogen activator activity and prostaglandin E₂ levels of human synovial cells

Interleukin‐1β and interleukin‐1α stimulate the plasminogen activator activity and prostaglandin E₂ levels of human synovial cells

Monocytes regulate osteoclast-activating factor production by releasing prostaglandins.

On the specificity of antisera against prostaglandins A₂ and E₂

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Radioimmunoassay Measurement of Prostaglandins E, A, and F in Human Plasma

Cited by 469 publications

References 44 publications

Interleukin‐1β and interleukin‐1α stimulate the plasminogen activator activity and prostaglandin E2 levels of human synovial cells

Interleukin‐1β and interleukin‐1α stimulate the plasminogen activator activity and prostaglandin E2 levels of human synovial cells

Monocytes regulate osteoclast-activating factor production by releasing prostaglandins.

On the specificity of antisera against prostaglandins A2 and E2

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Interleukin‐1β and interleukin‐1α stimulate the plasminogen activator activity and prostaglandin E₂ levels of human synovial cells

Interleukin‐1β and interleukin‐1α stimulate the plasminogen activator activity and prostaglandin E₂ levels of human synovial cells

On the specificity of antisera against prostaglandins A₂ and E₂