Radioimmunoassay for the Natural Plant Growth Regulator (−)-Jasmonic Acid

Knöfel, Hans‐Dieter; Brückner, Christian; Kramell, Robert; Sembdner, G.; Schréiber, Klaus

doi:10.1016/s0015-3796(11)80239-8

Cited by 48 publications

(20 citation statements)

References 15 publications

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“…JA was coupled to keyhole limpet hemocyanin and was used to raise rabbit antibodies specific for jasmonates as described (34). Specificity was assessed by determining the relative cross-reactivity to JA-amino acid conjugates.…”

Section: Methodsmentioning

confidence: 99%

Antisense-mediated depletion of a potato lipoxygenase reduces wound induction of proteinase inhibitors and increases weight gain of insect pests

Royo

León

Vancanneyt

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

158

111

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De novo jasmonic acid (JA) synthesis is required for wound-induced expression of proteinase inhibitors and other defense genes in potato and tomato. The first step in JA biosynthesis involves lipoxygenase (LOX) introducing molecular oxygen at the C-13 position of linolenic acid. We previously have shown that, in potato, at least two gene families code for 13-LOX proteins. We have now produced transgenic potato plants devoid of one specific 13-LOX isoform (LOX-H3) through antisense-mediated depletion of its mRNA. LOX-H3 depletion largely abolishes accumulation of proteinase inhibitors on wounding, indicating that this specific LOX plays an instrumental role in the regulation of wound-induced gene expression. As a consequence, weight gain of Colorado potato beetles fed on antisense plants is significantly larger than those fed on wild-type plants. The poorer performance of LOX-H3-deficient plants toward herbivory is more evident with a polyphagous insect; larvae of beet armyworm reared on the antisense lines have up to 57% higher weight than those fed on nontransformed plants. LOX-H3 thus appears to regulate gene activation in response to pest attack, and this inducible response is likely to be a major determinant for reducing performance of nonspecialized herbivores. However, the regulatory role of LOX-H3 is not caused by its involvement in the wound-induced increase of JA, as wild-type and LOX-H3 deficient plants have similar jasmonate levels after wounding. LOX-H3-deficient plants have higher tuber yields. The apparent effect of suppressing the inducible defensive response on plant vigor suggests that it may pose a penalty in plant fitness under nonstress situations.

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Section: Methodsmentioning

confidence: 99%

Antisense-mediated depletion of a potato lipoxygenase reduces wound induction of proteinase inhibitors and increases weight gain of insect pests

Royo

León

Vancanneyt

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

158

111

View full text Add to dashboard Cite

show abstract

“…The immunoreactive material was monitored using an antiserum raised against (Ϫ)-JA linked to hemocyanine (Knö fel et al, 1990). Based on the procedure described by Weiler (1986), ELISA was performed as described previously (Lehmann et al, 1995).…”

Section: Immunological Detection Of Jasmonatesmentioning

confidence: 99%

Octadecanoid-Derived Alteration of Gene Expression and the “Oxylipin Signature” in Stressed Barley Leaves. Implications for Different Signaling Pathways

et al. 2000

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Stress-induced gene expression in barley (Hordeum vulgare cv Salome) leaves has been correlated with temporally changing levels of octadecanoids and jasmonates, quantified by means of gas chromatography/mass spectrometry-single ion monitoring. Application of sorbitol-induced stress led to a low and transient rise of jasmonic acid (JA), its precursor 12-oxophytodienoic acid (OPDA), and the methyl esters JAME and OPDAME, respectively, followed by a large increase in their levels. JA and JAME peaked between 12 and 16 h, about 4 h before OPDA and OPDAME. However, OPDA accumulated up to a 2.5-fold higher level than the other compounds. Dihomo-JA and 9,13-didehydro-OPDA were identified as minor components. Kinetic analyses revealed that a transient threshold of jasmonates or octadecanoids is necessary and sufficient to initiate JA-responsive gene expression. Although OPDA and OPDAME applied exogenously were metabolized to JA in considerable amounts, both of them can induce gene expression, as evidenced by those genes that did not respond to endogenously formed JA. Also, coronatine induces JA-responsive genes independently from endogenous JA. Application of deuterated JA showed that endogenous synthesis of JA is not induced by JA treatment. The data are discussed in terms of distinct signaling pathways.

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“…The jasmonate was eluted with 80% acidic methanol. The eluate was concentrated and used for JA determination by ELISA according to the method of Knö fel et al (1990).…”

Section: Extraction and Analysis Of Jasmonatementioning

confidence: 99%

Inhibition of Wound-Induced Accumulation of Allene Oxide Synthase Transcripts in Flax Leaves by Aspirin and Salicylic Acid

1998

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Radioimmunoassay for the Natural Plant Growth Regulator (−)-Jasmonic Acid

Cited by 48 publications

References 15 publications

Antisense-mediated depletion of a potato lipoxygenase reduces wound induction of proteinase inhibitors and increases weight gain of insect pests

Antisense-mediated depletion of a potato lipoxygenase reduces wound induction of proteinase inhibitors and increases weight gain of insect pests

Octadecanoid-Derived Alteration of Gene Expression and the “Oxylipin Signature” in Stressed Barley Leaves. Implications for Different Signaling Pathways

Inhibition of Wound-Induced Accumulation of Allene Oxide Synthase Transcripts in Flax Leaves by Aspirin and Salicylic Acid

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