Radioimmunoassay for “free” testosterone in human saliva

Luisi, M; Bernini, Giampaolo; Genovese, A; Birindelu, R.; Barletta, D.; Gasperi, Maurizio; Franchi, F

doi:10.1016/0022-4731(80)90316-7

Cited by 42 publications

(12 citation statements)

References 13 publications

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“…Values closely approximating the male adult range were observed in the 17-yr-old group. This clear-cut, age dependence of salivary testosterone concentrations, also observed by others (57), emphasizes the need to establish normal ranges, based on decades of age, for the correct interpretation of clinical data.…”

Section: Agementioning

confidence: 77%

Steroids in Saliva for Assessing Endocrine Function

et al. 1982

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Section: Agementioning

confidence: 77%

Steroids in Saliva for Assessing Endocrine Function

et al. 1982

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“…However, higher [12,13] and lower [3,6] concentra tions have been reported. The mean level of T in female saliva was close to values pub lished previously [4,5], but higher levels [12,13] were also reported. The concentration of T in saliva and the calculated percent 'free' T index in the present study are quite close to the published estimation of free T in plasma [8], As a close correlation between salivary T and plasma-free T levels was reported in other studies [3,4,6,7], salivary T clearly seems to provide a reliable index of the con centration of free, unbound T in plasma.…”

Section: Discussionmentioning

confidence: 99%

“…7, 9] and mass spectrometry/gas chromatography methods [11]. However, higher [12,13] and lower [3,6] concentra tions have been reported. The mean level of T in female saliva was close to values pub lished previously [4,5], but higher levels [12,13] were also reported.…”

Section: Discussionmentioning

confidence: 99%

“…In this case, the RIA ofT in saliva and the calculated percent 'free' T in dex could represent a simple and accurate estimation of plasma free T levels available for target tissues, in clinical studies of male and female infertility and androgenic status. Preliminary investigations showed, in fact, the utility of salivary T determinations in case of male hypogonadism [6,12] prostatic cancer [15], thyroid diseases [6], hirsutism [ 1. 4.…”

Section: Discussionmentioning

confidence: 99%

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Further Evidence for the Usefulness of the Salivary Testosterone Radioimmunoassay in the Assessment of Androgenicity in Man in Basal and Stimulated Conditions

Magrini¹,

Chiodoni²,

Rey³

et al. 1986

Horm Res

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Since correct assessment of testicular function and androgenic status in humans requires multiple sampling, a sensitive and accurate radioimmunoassay (RIA) of testosterone (T) was established for male and female saliva samples. This easily collected biological fluid, which contains nonprotein-bound T, may represent an attractive alternative or a complement to total plasma T assays. In saliva samples from 5 normal males, a clear circadian rhythm was observed, and morning concentrations (135 ± 31 pg/ml) were significantly higher (p < 0.02) than evening samples (85 ± 23 pg/ml). In 11 normal females, morning saliva levels were 12.8 ± 1.8 pg/ml. The levels of T in male saliva, in response to both exogenous T administration (100 mg i.m.) and HCG stimulation (2 × 2,000 IU i.m.), accurately reflected the changes observed in plasma T, and the magnitude of increase in T levels was clearly greater in saliva than in plasma samples during the intramuscular administration of the long-acting T preparation. In males, significant correlations were observed between salivary and plasma T concentrations in morning samples (r = 0.61, p < 0.01), following HCG stimulation (r = 0.89, p < 0.05) and during T administration (r = 0.87, p < 0.05). In women, the correlation at 8 a.m. was also significant (r = 0.82, p < 0.05).

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“…1972;Nieschlag and Wickings, 1981). While T*.., is a measure of both the bound and the free testos terone, T«) provides a measurement of the free testosterone alone [Smith et al, 1979;Luisi et al. 1980;Wang et al, 1981].…”

Section: Hormone Assaysmentioning

confidence: 99%

Sex Hormones and Cognitive Functioning in Men

1987

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Blood and saliva samples were obtained from 117 healthy young men, following which radioimmunoassays were used to determine the serum concentrations of testosterone (T_ser), 5α-dihydrotestosterone (DHT), and the level of free testosterone (T_sal) in the saliva. The cognitive functioning was determined by five spatial and six verbal ipsative test scores, reflecting intra-individual variance in the performance of these tasks, independent of the person’s general level of achievement. Within the normal physiological range androgen levels – especially T_ser and to a lesser extent DHT and T_sal – showed a significantly positive correlation with measures of spatial ability and field dependence-independence and a significantly negative correlation with measures of verbal production.

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Radioimmunoassay for “free” testosterone in human saliva

Cited by 42 publications

References 13 publications

Steroids in Saliva for Assessing Endocrine Function

Steroids in Saliva for Assessing Endocrine Function

Further Evidence for the Usefulness of the Salivary Testosterone Radioimmunoassay in the Assessment of Androgenicity in Man in Basal and Stimulated Conditions

Sex Hormones and Cognitive Functioning in Men

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