Radioimmunoassay for Desmosine

King, Graham S.; Mohan, V. S.; Starcher, Barry

doi:10.3109/03008208009152362

Cited by 103 publications

(34 citation statements)

References 10 publications

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“…2). Immunology-based methods for DES detection in urine include ELISA [11] and radioimmunoassay (RIA) [12,13]. The latter method has undergone progressive modifications, after which urine did not require acid hydrolysis [14], and the specificity improved [6].…”

Section: Elastin Breakdown Products and Methods Of Measurementmentioning

confidence: 99%

“…Early work, in which urinary DES was detected by RIA [12], compared a group of both children and adults (the latter with or without emphysema) who had AATD (PI*ZZ genotype) with appropriate controls. Urinary DES in children (mean age 10 yrs) was significantly higher than it was in all adults (mean¡SD 3.50¡0.62 versus 2.32¡0.82 mg per 100 mg creatinine, respectively; p ,0.001), but levels in children with AATD were not different from those of age-matched controls [28].…”

Section: Urinary Des Levels In Normal Nonsmoking or Smoking Individuamentioning

confidence: 99%

“…The study lasted 24 weeks, and urinary DES excretion was measured weekly by two methods simultaneously, the isotope-dilution HPLC method [15] and RIA [12]. DES values showed a good correlation between the two methods of measurement but no significant differences occurred between values at entry and after 24 weeks of treatment.…”

Section: Urinary Des As a Surrogate End-point In Clinical Trials In Cmentioning

confidence: 99%

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Desmosine as a biomarker of elastin degradation in COPD: current status and future directions

Luisetti¹,

Ma²,

Iadarola³

et al. 2008

European Respiratory Journal

114

110

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Desmosine (DES) and isodesmosine (IDES) are two unusual, tetrafunctional, pyridinium ring-containing amino acids involved in elastin cross-linking. Being amino acids unique to mature, cross-linked elastin, they are useful for discriminating peptides derived from elastin breakdown from precursor elastin peptides. According to these features, DES and IDES have been extensively discussed as potentially attractive indicators of elevated lung elastic fibre turnover and markers of the effectiveness of agents with the potential to reduce elastin breakdown. In the present manuscript, immunology-based and separation methods for the evaluation of DES and IDES are discussed, along with studies reporting increased levels of urine excretion in chronic obstructive pulmonary disease (COPD) patients with and without a 1 -antitrypsin deficiency. The results of the application of DES and IDES as surrogate end-points in early clinical trials in COPD are also reported. Finally, recent advances in detection techniques, including liquid chromatography tandem mass spectrometry and high-performance capillary electrophoresis with laser-induced fluorescence, are discussed. These techniques allow detection of DES and IDES at very low concentration in body fluids other than urine, such as plasma or sputum, and will help the understanding of whether DES and IDES are potentially useful in monitoring therapeutic intervention in COPD.

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Section: Elastin Breakdown Products and Methods Of Measurementmentioning

confidence: 99%

Section: Urinary Des Levels In Normal Nonsmoking or Smoking Individuamentioning

confidence: 99%

Section: Urinary Des As a Surrogate End-point In Clinical Trials In Cmentioning

confidence: 99%

See 1 more Smart Citation

Desmosine as a biomarker of elastin degradation in COPD: current status and future directions

Luisetti¹,

Ma²,

Iadarola³

et al. 2008

European Respiratory Journal

114

110

View full text Add to dashboard Cite

show abstract

“…Because desmosine is unique to elastin in mammals (it is, however, found in avian and reptilian egg-shell membranes [26,27]), it can be used as a quantitative marker for insoluble elastin levels in tissues and cultured cells. Many methods have been employed for detection and quantification of desmosine, including HPLC [28][29][30][31][32][33], radioimmunoassay [27,[34][35][36][37], ELISA [38][39][40], capillary electrophoresis, [41] and mass spectrometry [42][43][44]. Of these techniques, only RIA and ELISA are applicable to high throughput analysis.…”

Section: Quantitation Of Desmosinementioning

confidence: 99%

Methods in elastic tissue biology: Elastin isolation and purification

Mecham

2008

Methods

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Elastin provides recoil to tissues subjected to repeated stretch, such as blood vessels and the lung. It is encoded by a single gene in mammals and is secreted as a 60-70 kDa monomer call tropoelastin. The functional form of the protein is that of a large, highly crosslinked polymer that organizes as sheets or fibers in the extracellular matrix. Purification of mature, crosslinked elastin is problematic because its insolubility precludes its isolation using standard wet-chemistry techniques. Instead, relatively harsh experimental approaches designed to remove non-elastin 'contaminates' are employed to generate an insoluble product that has the amino acid composition expected of elastin. Although soluble, tropoelastin also presents problems for isolation and purification. The protein's extreme stickiness and susceptibility to proteolysis requires careful attention during purification and in tropoelastin-based assays. This article describes the most common approaches for purification of insoluble elastin and tropoelastin. It also addresses key aspects of studying tropoelastin production in cultured cells, where elastin expression is highly dependent upon cell type, culture conditions, and passage number.

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“…While the RIA provides reproducible results and the specificity of the antibody for desmosine has been documented in both urine and lung homogenates, 19,33 recent methodological advances 34 in liquid chromatography and tandem mass spectrometry may allow for more sensitive and specific measurements of desmosine for future studies.…”

Section: Discussionmentioning

confidence: 99%

Sputum Desmosine During Hospital Admission for Pulmonary Exacerbation in Cystic Fibrosis

Laguna

Wagner

Luckey

et al. 2009

Chest

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Background: Cystic fibrosis (CF) lung disease is characterized by structural changes in the airways and parenchyma. No sputum biomarker exists to measure the degree of active structural destruction during pulmonary exacerbation in patients with CF. The noninvasive measurement of desmosine, a breakdown product of elastin, may reflect ongoing lung injury and serve as a biomarker of short-term damage. Our objectives were to measure desmosine in the sputum of patients with CF hospitalized for treatment of a pulmonary exacerbation and to explore the correlation between desmosine levels and other markers of clinical improvement, including lung function and inflammatory mediators, following hospitalization. Methods: Sputum and blood samples collected and lung function measurements were made at multiple time points during hospitalization. We used a repeated measures model, adjusted for age and time between measurements, to compare log-transformed sputum desmosine levels across multiple time points and to correlate those levels with related variables.

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Radioimmunoassay for Desmosine

Cited by 103 publications

References 10 publications

Desmosine as a biomarker of elastin degradation in COPD: current status and future directions

Desmosine as a biomarker of elastin degradation in COPD: current status and future directions

Methods in elastic tissue biology: Elastin isolation and purification

Sputum Desmosine During Hospital Admission for Pulmonary Exacerbation in Cystic Fibrosis

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