Radioimmunoassay for brassinosteroids and its use for comparative analysis of brassinosteroids in stems and seeds ofPhaseolus vulgaris

Yokota, Takao; Watanabe, Satoru; Ogino, Yasuyo; Yamaguchi, Isomaro; Takahashi, Nobutaka

doi:10.1007/bf02041955

Cited by 52 publications

(18 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ultrathin sections were collected on gold grids (200 mesh) and incubated in a mixture of 1% bovine serum albumin (BSA) and 0.1% Tween 20 in 10 mM phosphate-buffered saline (PBS; mono-and dibasic phosphate buffer with 150 mM NaC1, pH 7.4) followed by BSA-PBS, then incubated for 1 h in serum containing polyclonal antibodies to castasterone raised in rabbit (Yokota et al 1990). The serum was diluted 1:200 (w/v) from freeze-dried material.…”

Section: Methodsmentioning

confidence: 99%

“…As the antibodies recognise brassinolide, castasterone, and 3-epicastasterone, and there is 20-50 % cross-reactivity to seven other natural brassinosteroids (Yokota et al 1990), other chemical identification of the particular brassinosteroids present in rye-grass pollen would be useful. Therefore, partially purified extracts were analysed by high-performance liquid chromatography (HPLC) with fluorescence detection of dansylaminophenylboronate derivatives (Gamoh et al 1990).…”

Section: Introductionmentioning

confidence: 99%

“…Abe (1991) has reported evidence for the synthesis of brassinosteroids in the male reproductive tissue of lily; as the tissue matured, levels of typhasterol increased and so did the relative proportion present of the more bioactive brassinolide and castasterone. Subcellular localization of brassinosteroids could assist in determining their function in pollen, and the availability of polyclonal antibodies to castasterone (Yokota et al 1990) prompted us to attempt the detection of active brassinosteroids in both mature and developing pollen by immunocytochemistry.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Detection of brassinosteroids in pollen of Lolium perenne L. by immunocytochemistry

Taylor

Spuck

Smith

et al. 1993

Planta

Self Cite

View full text Add to dashboard Cite

Abstract. Bioactive brassinosteroids have been localized in developing and mature pollen of anhydrously fixed rye-grass (Lolium perenne) by immunocytochemistry using polyclonal antibodies to castasterone generated in rabbits. Tricellular pollen fixed by freeze-substitution was also labelled in the starch granules. Study of the developmental sequence of the pollen through the microsporocyte, microspore, bicellular and tricellular stages showed that the brassinosteroids were increasingly sequestered in starch granules as the amyloplasts matured, supporting the view that these are storage organelles for these potent plant growth promoters. In bicellular pollen, heavy labelling was seen in the zone within 0.5 gm of the starch granule, where stromal tissue remains. Thus, the stroma may be the site of synthesis of these compounds. During aqueous fixation, the brassinosteroids leached from the starch granules of tricellular pollen, indicating that they would be quickly available after imbibition to influence the physiology of germinating pollen. The results from high-performance liquid chromatography of dansylaminophenylboronates from partially purified extracts of freshly dehisced tricellular pollen of rye-grass showed 25-methylcastasterone may be a minor component, together with two unknown peaks. No specific binding of brassinolide to any soluble proteins extracted from tricellular rye-grass pollen was observed using the antibodies in gel electrophoresis or enzyme-linked immunosorbent assays.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of brassinosteroids in pollen of Lolium perenne L. by immunocytochemistry

Taylor

Spuck

Smith

et al. 1993

Planta

Self Cite

View full text Add to dashboard Cite

show abstract

“…For investigations of biosynthesis, metabolism, transport and distribution especially the preparation of radioactive labelled brassinosteroids is important, whereas only few studies in this direction have been done hitherto.Thus, starting from dolicholide via catalytically tritiation [24,28-3H2]-brassinolide and [24,28-3H2]-24-epibrassinolide were prepared [4]. Also a synthetic pathway leading to [4-1%]-24-epibrassinolide has been published [5].…”

Section: Introductionmentioning

confidence: 98%

Synthesis of tritium labelled 24‐epibrassinolide

Kolbe

Marquardt

Adam

1992

Labelled Comp Radiopharmac

View full text Add to dashboard Cite

SUMMARYDeuterium and tritium 5,7,7-tris-labelled 24-epibrassinolide were prepared by base catalyzed exchange reaction using 24-epicastasterone tetraacetate I or bis-isopropylidenedioxy-24-epicastasterone 8 and labelled water. Baeyer-Villiger oxidation of the obtained labelled 6-ketones 2 and 3 with CF3C03H gave after alkaline deacetylation of the resulting 4 and 5 the desired tris-labelled 24-epibrassinolides 6 and 2, respectively, or starting from 9 under simultaneous oxidation and deprotection in one step the same final products.

show abstract

“…6 ) [24, 28-3 H]CS (sp. act., 8.3 x 10 13 Bq mol-1 ) thus obtained was homogenous on reversedphase HPLC and silica gel TLC.…”

mentioning

confidence: 99%

Brassinolide Is Biosynthesized from Castasterone inCatharanthus roseusCrown Gall Cells

Yokota

Ogino

Takahashi

et al. 1990

Agricultural and Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Radioimmunoassay for brassinosteroids and its use for comparative analysis of brassinosteroids in stems and seeds ofPhaseolus vulgaris

Cited by 52 publications

References 16 publications

Detection of brassinosteroids in pollen of Lolium perenne L. by immunocytochemistry

Detection of brassinosteroids in pollen of Lolium perenne L. by immunocytochemistry

Synthesis of tritium labelled 24‐epibrassinolide

Brassinolide Is Biosynthesized from Castasterone inCatharanthus roseusCrown Gall Cells

Contact Info

Product

Resources

About