Radioimmunoassay for Bovine and Ovine Luteinizing Hormone<sup>1</sup>

Niswender, Gordon D.; Reichert, Leo E.; Midgley, A. Rees; Nalbandov, A. V.

doi:10.1210/endo-84-5-1166

Cited by 838 publications

(267 citation statements)

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“…Blood samples were allowed to clot at room temperature for 6 h, stored at 4°C and the following day serum was collected. A rapid radioimmunoassay for LH was performed using 200 µ serum, 100 µ 125I-labelled oLH, 200 µ anti-ovine LH serum (Niswender et a!, 1969) and 500µ phosphate-buffered saline containing 01% gelatin (PBS-gel; 001 M-NaP04, 014M-NaCl, 0-22min-merthiolate, 0-1 % (w/v) gelatin; pH 70) and were incubated for 8 h at 4°C. The 200 µ of second antibody (goat anti¬ rabbit immunoglobulin) were added to each assay tube and they were incubated for 12 additional hours.…”

Section: Methodsmentioning

confidence: 99%

Functional and morphological characteristics of the first corpus luteum formed after parturition in ewes

Braden¹,

Sawyer²,

Niswender³

1989

Reproduction

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Section: Methodsmentioning

confidence: 99%

Functional and morphological characteristics of the first corpus luteum formed after parturition in ewes

Braden¹,

Sawyer²,

Niswender³

1989

Reproduction

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“…When housed on the dry lot, the ewes were fed hay. All animals were given (Niswender et al, 1969) modified as described previously (Legan et al, 1985a). The limit of detection (95% confidence limit of maximum binding) averaged 011 ng/ml for 200 µ serum.…”

Section: Methodsmentioning

confidence: 99%

Changes in LH pulse frequency and serum progesterone concentrations during the transition to breeding season in ewes

I’Anson

Legan²

1988

Reproduction

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Summary. To characterize the changes in LH pulse frequency during the transition to breeding season, LH pulse patterns and serum progesterone profiles were determined in 8 intact ewes from mid-anoestrus to the early breeding season. Overall, 8 increases in LH pulse frequency were observed and these were restricted to 5 ewes. Of the 8 increases, 7 occurred during the 4 weeks before the first cycle, 5 of them within 1 week after a pulse frequency typical of anoestrus (0\p=n-\2per 8 h). Six of them occurred less than 1 week before either a full-length luteal phase (n = 2) or a 1\p=n-\3-day increment in progesterone (n = 4). Seven of these brief progesterone increases were observed in 6 ewes, 5 of them immediately preceding the first full-length luteal phase. These results are consistent with the hypothesis that the seasonal decrease in response to oestradiol negative feedback at the beginning of the breeding season causes an increase in GnRH, and thereby LH pulse frequency. In addition, they demonstrate that the first increase in tonic LH secretion occurs in < 1 week and, in most ewes, initiates either the first fulllength cycle or a transient increase in progesterone, the latter occurring more often.

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“…A double antibody precipitation method [14], as modified [15], was used for radioimmunoassay. '251-labeled pit-bGH …”

Section: Radioimmunoassaymentioning

confidence: 99%

Recombinant‐DNA‐derived bovine growth hormone from Escherichia coli

Langley

Lai

Wypych

et al. 1987

European Journal of Biochemistry

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Bacterially synthesized, recombinant‐DNA‐derived bovine growth hormone (r‐bGH), prepared as described in the preceding paper in this journal, has been characterized in comparison with pituitary bovine growth hormone (pit‐bGH). The characterization criteria include sodium dodecyl sulfate/polyacrylamide gel electrophoresis, automated N‐terminal sequence analysis, amino acid composition, isoelectric focusing, reverse‐phase high‐performance liquid chromatography, ultraviolet absorbance, analysis for free protein thiol, sizing by gel filtration, circular dichroism, radioimmunoassay and biological activity in the hypophysectomized rat weight‐gain assay. In every respect the r‐bGH appears to be virtually identical to pit‐bGH.

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Radioimmunoassay for Bovine and Ovine Luteinizing Hormone¹

Cited by 838 publications

References 0 publications

Functional and morphological characteristics of the first corpus luteum formed after parturition in ewes

Functional and morphological characteristics of the first corpus luteum formed after parturition in ewes

Changes in LH pulse frequency and serum progesterone concentrations during the transition to breeding season in ewes

Recombinant‐DNA‐derived bovine growth hormone from Escherichia coli

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Radioimmunoassay for Bovine and Ovine Luteinizing Hormone1

Cited by 838 publications

References 0 publications

Functional and morphological characteristics of the first corpus luteum formed after parturition in ewes

Functional and morphological characteristics of the first corpus luteum formed after parturition in ewes

Changes in LH pulse frequency and serum progesterone concentrations during the transition to breeding season in ewes

Recombinant‐DNA‐derived bovine growth hormone from Escherichia coli

Contact Info

Product

Resources

About

Radioimmunoassay for Bovine and Ovine Luteinizing Hormone¹