Radioimmunoassay analysis of baculovirus granulins and polyhedrins

Summers, Max D.; Hoops, Pepper

doi:10.1016/0042-6822(80)90128-2

Cited by 12 publications

(3 citation statements)

References 29 publications

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“…Virus-specific proteins were precipitated from the cell-free translation mixture with serum prepared against either purified polyhedrin or occluded virus proteins of AcMNPV. The antisera were prepared essentially as described by Summers and Hoops (21). Cross-reactions between occluded virus proteins and serum prepared against purified polyhedrin did not occur, as determined by the new immunoautoradiographic techinque of Symington et al (24), as modified by Smith and Summers (19a).…”

Section: Methodsmentioning

confidence: 99%

Hybridization Selection and In Vitro Translation of Autographa californica Nuclear Polyhedrosis Virus mRNA

Vlak

Smith

Summers

1981

J Virol

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We isolated polyadenylated RNA from the cytoplasm of cells infected with Autographa californica nuclear polyhedrosis virus late after infection (21 h postinfection). At that time intracellular protein synthesis was directed almost exclusively toward infected cell-specific proteins. The polyadenylic acid-containing RNA sequences in the cytoplasm at 21 h postinfection were radiolabeled in vitro and hybridized to A. californica nuclear polyhedrosis virus DNA restriction fragments. The polyadenylic acid-containing RNA was derived from regions representing the entire viral genome. Translation in a reticulocyte cell-free protein-synthesizing system of cytoplasmic RNA selected by hybridization to viral DNA and polyadenylic acid-containing RNA produced almost identical polypeptide patterns, suggesting that late after infection almost all of the cytoplasmic polyadenylic acid-containing RNA present in infected cells was of viral origin. Polyhedrin protein (molecular weight, 33,000) and a number of virion structural proteins were among the translation products which were identified by immunoprecipitation and by comparing molecular weights. In addition, some tentative nonstructural infected cell-specific proteins were also detected. Using the hybridization selection technique, we determined that sequences complementary to the message coding for polyhedrin were located on EcoRI fragment I of A. californica nuclear polyhedrosis virus DNA, whereas sequences coding for a putative nonstructural protein (molecular weight, 39,000) were on EcoRI fragment J. Nuclear polyhedrosis viruses are members of subgroup A in the family Baculoviridae. A distinctive feature of these invertebrate DNA viruses is the occlusion of many enveloped virus particles in a large paracrystalline inclusion, which is referred to as a polyhedron. About 95% of the polyhedron mass is composed of a single polypeptide having a molecular weight of approximately 30,000, which is referred to as poly

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Section: Methodsmentioning

confidence: 99%

Hybridization Selection and In Vitro Translation of Autographa californica Nuclear Polyhedrosis Virus mRNA

Vlak

Smith

Summers

1981

J Virol

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“…Although antigenic data indicate relatively close relatedness between granulin and polyhedrin protein (Smith & Summers, 1981 ;Summers & Hoops, 1980) only about 50 ~o of the amino acid sequence is in fact homologous. Certain regions of these proteins, however, are highly conserved and probably contain the antigenic sites that result in the serological cross-reactivity.…”

Section: Relatedness Of Polyhedrin Genesmentioning

confidence: 99%

“…The nucleocapsid proteins of GVs and NPVs have been compared on the basis of their size distribution (Summers & Smith, 1978) and antigenic relatedness using Western blots (Smith & Summers, 1981). The antigenic relatedness of polyhedrins and granulins has been examined using radioimmunoassay (Summers & Hoops, 1980), In addition, the DNA sequence homology of a variety of baculoviruses has been compared by DNA-DNA hybridization (Smith & Summers, 1982). These various studies have not provided a clear understanding of the relatedness of different GVs to one another or their relatedness to NPVs.…”

Section: Introductionmentioning

confidence: 99%

The Nucleotide Sequence of the Pieris brassicae Granulosis Virus Granulin Gene

Chakerian

Rohrmann

Nesson

et al. 1985

Journal of General Virology

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SUMMARYTwo overlapping restriction fragments containing the Pieris brassicae granulosis virus (GV) granulin gene were cloned into plasmids. The regions containing the coding region and the 5" and 3' flanking regions were subcloned into M 13 and sequenced. The nucleotide sequence data were compared to those for the granulin gene from the Trichoplusia ni GV and the polyhedrin gene from the Autographa californica nuclear polyhedrosis virus (AcMNPV). The amino acid sequences derived from these DNA sequences indicated that the two GV proteins are more closely related to each other (77~ amino acid homology) than either is to the AcMNPV (about 53~ amino acid homology for either GV). The N-terminal region shows the greatest degree of variation between these proteins. Highly conserved amino acid sequences were identified between the two GVs and were also found between NPVs. Certain of these conserved regions are shared between GVs and NPVs while others are not.

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Descriptions of Virus Families and Groups

Francki

Fauquet

Knudson

et al. 1991

Classification and Nomenclature of Viruses

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Alfalfa mosaic 392 Microviridae 178 Arenaviridae 284 Myoviridae 161 Baculoviridae 117 Necrovirus 316 Birnaviridae 200 Nepovirus 368 Bromovirus 382 Nodaviridae 372 Bunyaviridae 273 Orthomyxoviridae 263 Caliciviridae 300 Papovaviridae 146 Capillovirus 339 Paramyxoviridae 242 Carla virus 341 Parsnip yellow fleck 318 Carmovirus 303 Partitiviridae 208 Caulimovirus 150 Parvoviridae 167 Closterovirus 345 Pea enation mosaic 375 Commelina yellow mottle

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Radioimmunoassay analysis of baculovirus granulins and polyhedrins

Cited by 12 publications

References 29 publications

Hybridization Selection and In Vitro Translation of Autographa californica Nuclear Polyhedrosis Virus mRNA

Hybridization Selection and In Vitro Translation of Autographa californica Nuclear Polyhedrosis Virus mRNA

The Nucleotide Sequence of the Pieris brassicae Granulosis Virus Granulin Gene

Descriptions of Virus Families and Groups

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