Radiographic, macromorphological and hematological evaluation of hydroxyapatite ceramics with diﬀ erent physicochemical properties

Chemerovsky, V.

doi:10.33245/2310-4902-2020-154-1-140-152

Cited by 7 publications

(4 citation statements)

References 13 publications

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“…Водночас рівень вимог [27][28][29][30] до композитних матеріалів постійно підвищується, вони мають характеризуватися наступними властивостями: біосумісністю; здатністю до біорезорбції та поступового заміщення кістковою тканиною з швидкою остеоінтеграцією; остеокондуктивністю та остеоіндуктивністю; відносною простотою введення у місце дефекту та можливістю моделювання під час оперативного втручання. Найбільшою мірою цим вимогам відповідають наступні групи матеріалів: 1) біоорганічні -демінералізований кістковий матрикс, колаген, фібринові композити; 2) керамічні матеріали -оксиди алюмінію та цирконію, гідроксиапатитні композити; 3) синтетичні полімери -поліметилметакрилат, полігліколід, полідіоксанон; 4) багатокомпонентні різних типів [31,32]. Заразом в умовах клінічної практики суттєвим є врахування об'ємів і особливостей кісткової травми, ступеня втрати кістковою тканиною регенеративного потенціалу.…”

Section: хірургія та анестезіологіяunclassified

Comparison of osseointegration properties of individual and standard sized implants

Ulianchych,

Firstov,

Kolomiiets

et al. 2023

Nauk. vìsn. vet. med.

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According to clinical and radiological studies,the presented samples of calcium-phosphate ceramicimplants fully perform the osteoconductive function.However, in the case of their dense contact with theedges of the bone defect (experimental group), the primary inflammatory-resorptive stage of reparative osteogenesis turned out to be more intense and prolongedin time with pronounced phenomena of osteoresorptionof the mother bone and volumetric periosteal reactionas a compensatory mechanism. At the same time, osteoid formation is formed around the implant, while thecontrol implants are partially resorbed and replaced byosteoid tissue. As a result, macromorphologically, experimental implants in compact bone are visualized upto the 42nd day, and in cancellous bone - up to the 30thday, while the control implants are covered with boneregenerate already on the 14th day, which in the lattercase indicates the presence of osseointegration processes. The histological picture in the case of experimentalimplants confirms the prolongation of the inflammatory-resorptive stage with the formation of cartilage-boneregenerate around them, i.e. the dense placement of theimplants causes biomechanical pressure on the walls ofthe bone defect with the development of a reaction inthem, primarily to a foreign body.The study of the effect on reparative osteogenesis of the osseointegration properties of the developednew individual and standard-sized bioactive ceramicimplants based on clinical-radiological, macromorphological and histomorphological justification is relevant,as it will make it possible to evaluate their advantagesin terms of consolidation of complex defective fractures of tubular bones and reduce the frequency of theircomplications in animals. Key words: rabbits, bioceramics, reparative osteogenesis, bone defects.

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Section: хірургія та анестезіологіяunclassified

Comparison of osseointegration properties of individual and standard sized implants

Ulianchych,

Firstov,

Kolomiiets

et al. 2023

Nauk. vìsn. vet. med.

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“…Найскладнішими серед травм опорно-рухового апарату як у людини, так і у домашніх тварин є осколкові переломи довгих трубчастих кісток, за яких застосування лише методів остеосинтезу не завжди забезпечує оптимальний перебіг репаративної регенерації кісткової тканини, оскільки за наявності багатоплощинної лінії злому та кісткових дефектів втрачається її регенеративний потенціал насамперед з причин втрати остеокондуктивності та порушень локальної гемодинаміки і мікроциркуляції (O'Neill et al, 2018). Кісткові дефекти, які виникають за такого виду переломів, зумовлюють збільшення кількості післяопераційних ускладнень, а надалі можливі розлади статико-динамічної функції травмованої кінцівки і навіть зміни загального стану організму тварини в цілому (Brusko & Gajko, 2005;Chemerovskyi, 2020).…”

Section: вступunclassified

“…На сьогодні розроблено і представлено на ринку низку композитних матеріалів вітчизняного і зарубіжного виробництва на основі гідроксиапатиту, α-чи β-трикальційфосфату, які за своїм хімічним складом та механічними властивостями максимально ідентичні до мінерального компоненту кісткової тканини (Chemerovskyi, 2020).…”

Section: вступunclassified

“…У випадку надання їм остеоіндуктивних властивостей вони потребують оцінки не тільки місцевої, а й загальної реакції організму. Зокрема, як було вказано в ряді робіт (Rublenko et al, 2018;Chemerovskyi, 2020), за використання гідроксиапатиту, легованого кремнієм, репаративний остеогенез прискорюється за рахунок скорочення запально-резорбтивної стадії та прискорення проліферативної, головним чином з боку ендоосту з ранньою мінералізацією кісткового регенерату. При цьому динаміка гематологічних показників є типовою для консолідації довгих трубчастих кісток, що свідчить про відсутність вираженої загальної реакції організму на імплантацію гідроксиапатитної кераміки, легованої кремнієм.…”

Section: обговоренняunclassified

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Dynamics of hematological parameters in rabbits for osteosubstitution by hydroxyapatite ceramics doped with germanium and in complex with blood coagulation activator

2021

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Bone tissue has powerful regenerative properties, thanks to which, with stable fixation, quite large amounts of skeletal bone damage can be successfully repaired. However, in the case of fragmentary fractures, the use of osteosynthesis methods alone does not always ensure the optimal course of reparative regeneration, as its regenerative potential is lost. Therefore, there is a need to replace post-traumatic bone defects and stimulate reparative osteogenesis. For this purpose, doped or doped with various elements (Ge, Si, Zn, Ag, Cu) composite materials. The aim of the study was to investigate the dynamics of hematological parameters in rabbits for osteosubstitution by hydroxyapatite ceramics doped with germanium and in combination with a blood clotting activator. Model defects were formed in the radial diaphysis and femur metaphysis in rabbits with a 3 mm and 4.2 mm diameter drill bit, respectively. Anesthesia included acepromazine, thiopenate, and lidocaine infiltration anesthesia. Animals of the first experimental group (n = 12) were replaced by defects with granules of hydroxyapatite ceramics doped with germanium (HTGe), the second (n = 12) – hydroxyapatite ceramics doped with germanium with blood coagulation activator (HTGe + a), the third – (n = 12). hydroxyapatite α + β with active (α + β + a), and control – granules of undoped ceramics (HT). On day 7, animals of all groups had minor post-traumatic erythrocytopenia and oligochromemia. There was also a gradual increase in the content of leukocytes in the blood with a peak on the 14th day, which in the group HTGe + a lasted until the 30th day. The increase in the number of leukocytes and their peak values occurred within the physiological norm and only approached its upper limit. In most groups there was a pronounced thrombocytosis during the first 14 days with normalization to the 30th day, but in the 2nd experimental group the number of platelets returned to normal only on the 60th day. Changes in most integral hematological indices, which reflect the relationship between blood cell populations, are characteristic of the inflammatory-resorptive phase of reparative osteogenesis. The dynamics of hematological parameters indicates the absence of a pronounced reaction of the body to the implantation of hydroxyapatite composite doped with germanium. The combination of hydroxyapatite ceramics doped with germanium with coagulation activator eliminates post-traumatic oligochromemia and erythrocytopenia and significantly increases the level of hematological integral indices, which indicates a more intensive course of inflammatory-resorptive clinical resorptive aparation phase.

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Effect of implants of hydroxyapatite with tricalcium phosphates alloyed with Si on histomorphological and biochemical parameters in cases of bone defects of rabbits

Chemerovskіy

Rublenko

et al. 2021

Regul. Mech. Biosyst.

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Complex comminuted fractures are accompanied by development of bone defects and loss of reparative potential of the bone tissue in the region of the trauma. This brings the necessity of using implants with optimum osteoconductive and osteointegration properties. The objective of the study was determining the condition of biochemical bone markers and peculiarities of histomorphological changes under the influence of ceramic hydroxyapatite (HA) implants with various physical-chemical properties in the conditions of diaphyseal bone defects in rabbits. We composed control and experimental groups of rabbits with 10 individuals in each with diaphyseal bone defects (3 mm) of the radial bones formed under general anesthesia. In one experimental group, they were filled with granules of hydroxyapatite with α-tricalcium phosphate, and in the second group – with β-tricalcium phosphate, alloyed with Si. In the control rabbits, the defects healed under a blood clot. Blood was analyzed on the 3rd, 7th, 14th, 21st and 42nd days, and as reference we used biochemical parameters of blood of clinically healthy rabbits (n = 10). Bone biopsied materials were taken on days 21–42 under general anesthesia. When using hydroxyapatite with β-tricalcium phosphate, alloyed with Si, we determined early intensification of the levels of nitrogen oxide, angiogenesis and development of bone regenerate in conditions of shortening of inflammatory resorption phase, which was verified according to the level of tartrate-resistant acid phosphatase. According to the level of bone isoenzyme of alkaline phosphatase in the blood serum of animals of the control group, the reparative osteogenesis developed slowly and peaked on day 42, whereas in animals implanted with α-tricalcium phosphate, its development peaked peaked on days 14–42, and when using Si-alloy – on days 7–14. Histomorphologically, on the 21st day, in the case of replacement of bone defect with hydroxyapatite with α-tricalcium phosphate, coarse-fibered type of bone regenerate developed with no dense contact with the elements of the regenerate, while spongy bone trabeculae occurred when hydroxyapatite was applied with β-tricalcium phosphate alloyed with Si, and the control rabbits were observed to be in the stage of cartilaginous callus. On the 42nd day, under the influence of implants of hydroxyapatite with α-tricalcium phosphate, the spongy bone tissue transformed into compact tissue with further mineralization. With implants alloyed with Si, there occurred compact bone tissue, and bone regenerates of the control animals were regions of coarse-fibered and spongy bone tissue without dense contact with the parent bone. This study revealed that hydroxyapatite with β-tricalcium phosphate alloyed with Si had notable osteoinductive and osteointegrating properties, as indicated by early angiogenesis and osteoblast reaction, positive dynamics of the marker biochemical parameters with faster and better development of bone regenerate as spongy bone trabeculae.

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Radiographic, macromorphological and hematological evaluation of hydroxyapatite ceramics with diﬀ erent physicochemical properties

Cited by 7 publications

References 13 publications

Comparison of osseointegration properties of individual and standard sized implants

Comparison of osseointegration properties of individual and standard sized implants

Dynamics of hematological parameters in rabbits for osteosubstitution by hydroxyapatite ceramics doped with germanium and in complex with blood coagulation activator

Effect of implants of hydroxyapatite with tricalcium phosphates alloyed with Si on histomorphological and biochemical parameters in cases of bone defects of rabbits

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