Purpose: To show that radiation response across cancer cell lines of the same anatomic site and histologic type varies remarkably for protons and carbon (C) ions. Materials and Methods: We measured and obtained from the literature clonogenic survival of human cancer cell lines of the lung (n=18), brain (n=10) and pancreas (n=10) exposed to photons, protons, and C-ions to assess their variability in response. We also treated cancer cell lines with DNA repair inhibitors prior to irradiation to assess how DNA repair capacity affects their variability in response. We quantified the variability in response by calculating the relative range (range/mean) and the coefficient of variation (COV) of the dose at 10% survival fraction (D10%) and relative biological effectiveness (RBE10%). Results: The relative range of D10% for lung cancer cell lines varied from 55-92% for photons, protons, and Cions, with the relative range in RBE varying from 16-45% for protons and C-ions. For brain and pancreatic cancer cell lines, the relative range of D10% varied from 95-112%, and 39-75%, respectively, with the relative range in RBE varying from 27-33% and 25-50%, respectively. However, the COVs in D10% were approximately equal across radiation qualities, varying from 0.24±0.07-0.35±0.10, 0.35±0.09-0.69±0.62 and 0.13±0.03-0.21±0.04 for lung, brain and pancreatic cancer cell lines, respectively. Greater relative ranges in D10% were observed in the cell lines with inhibited DNA repair, varying from 108%-157% for photons, protons, and C-ions, with relative ranges in RBE varying from 29-67%. The COVs in the D10% were also greater for the cell lines treated with inhibitors of DNA repair, varying from 0.34±0.09-0.41±0.06. Conclusions: Cell lines of the same anatomic site and histologic type have a remarkable variability in response, not only to photons but also to protons and C-ions. We attributed this variability to differences in DNA repair capacity.
Category: Biological Physics and Response PredictionClonogenic cell survival assay Cells were trypsinized 18-24 hours prior to irradiation and seeded into 6-well plates or T-12.5 flasks at appropriate numbers for each dose. Cell lines were allowed to form colonies for 7-14 days and then were fixed and stained with pure ethanol containing 0.5% crystal violet. Plates were allowed to air dry overnight and then scanned using a high-resolution flatbed scanner (Epson Expression 10000 XL). Images were analyzed using an ImageJ plugin developed in-house that automatically counts colonies containing more than 50 cells. 24 Details of the clonogenic cell survival assay are in Supplementary Note 3.
Statistical analysesStatistical analyses were performed in MATLAB 2017 (Mathworks, Natick, MA) and Graph Pad Prism 7 (Graph Pad, San Diego, CA). Error bars represent the standard error propagated from the survival curve parameters (α and β) fits, including their covariance, to the parameter estimates.
RESULTSWe found large variability in the D10% values of several lung, brain and pancreatic cancer cell lines to ph...