Radiochromatography in pharmaceutical and biomedical analysis

Veltkamp, A.C.

doi:10.1016/s0378-4347(00)82282-3

Cited by 13 publications

(6 citation statements)

References 68 publications

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“…Radiolabels, as a tracer of drug related components, not only facilitate the detection, isolation and identification of radioactive metabolites, but also allow the parent drug and its metabolites to be quantified without the use of synthetic standards. Various radiochromatographic techniques are employed for metabolite profiling in biological matrices [5] and for metabolite concentration determination in enzyme kinetic consists of four steps: HPLC separation, fraction collection into test tubes, mixing with scintillation cocktail, and counting radioactivity one fraction a time. The entire process is time-consuming and labor-intensive.…”

Section: Introductionmentioning

confidence: 99%

Analysis of low level radioactive metabolites in biological fluids using high-performance liquid chromatography with microplate scintillation counting: Method validation and application

Zhu¹,

Zhao²,

Vázquez³

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

Analysis of low level radioactive metabolites in biological fluids using high-performance liquid chromatography with microplate scintillation counting: Method validation and application

Zhu¹,

Zhao²,

Vázquez³

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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“…One problem associated with on-line radiochemical detection is that relatively high-volume detector cells have to be used to obtain acceptable sensitivity (14): this results in peak broadening. In the present study the resolution in the radiochemically detected chromatograms was not good enough for quantitation of individual glycosides and sulfates; only a value representing their sum was therefore calculated.…”

Section: Discussionmentioning

confidence: 99%

Metabolite profiles of two [14C]-labelled catechol O-methyltransferase inhibitors, nitecapone and entacapone, in rat and mouse urine and rat bile

Wikberg

Vuorela

1994

Eur. J. Drug Metab. Pharmacokinet.

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The metabolites of two inhibitors of catechol O-methyltransferase, nitecapone [3-(3,4-dihydroxy-5-nitrobenzylidene)2,4-pentanedione] and entacapone [(E)-2-cyano-N,N-diethyl-3-(3,4-dihydroxy-5-nitrophenyl)propenamide++ +], excreted in urine and bile by rats and in urine by mice, were compared and quantified by using HPLC with radiochemical detection after administration of [14C]-labelled compounds. With the exception of 3-O-methylated nitecapone, no major metabolites were found in rat bile that were not found in rat urine. For both compounds the major biotransformations were the same in the mouse and the rat. However, a bisulfite adduct of nitecapone was found in rat urine only, and reduction of the C = C and C = O groups of the nitecapone side chain was more extensive in the mouse. After entacapone administration, the products of amide N-dealkylation were more abundant in rat urine than in mouse urine. Most of the dose was excreted in urine and bile as O-conjugates. Most abundant were the O-glucuronides, while smaller amounts of O-sulfates and O-methylated metabolites were found in both species. One non-glucuronide glycoside of entacapone was found in urine of both rats and mice.

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“…Radio flow detection is a versatile and high sensitive technique suitable for many applications [ 14 – 17 ]. The radioactivity detected is directly proportional to the concentration of the analyte according to its speciation.…”

Section: Introductionmentioning

confidence: 99%

A LabVIEW®-based software for the control of the AUTORAD platform: a fully automated multisequential flow injection analysis Lab-on-Valve (MSFIA-LOV) system for radiochemical analysis

Barbesi

Vilas

Millet

et al. 2017

J Radioanal Nucl Chem

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A LabVIEW®-based software for the control of the fully automated multi-sequential flow injection analysis Lab-on-Valve (MSFIA-LOV) platform AutoRAD performing radiochemical analysis is described. The analytical platform interfaces an Arduino®-based device triggering multiple detectors providing a flexible and fit for purpose choice of detection systems. The different analytical devices are interfaced to the PC running LabVIEW®VI software using USB and RS232 interfaces, both for sending commands and receiving confirmation or error responses. The AUTORAD platform has been successfully applied for the chemical separation and determination of Sr, an important fission product pertinent to nuclear waste.

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Radiochromatography in pharmaceutical and biomedical analysis

Cited by 13 publications

References 68 publications

Analysis of low level radioactive metabolites in biological fluids using high-performance liquid chromatography with microplate scintillation counting: Method validation and application

Analysis of low level radioactive metabolites in biological fluids using high-performance liquid chromatography with microplate scintillation counting: Method validation and application

Metabolite profiles of two [14C]-labelled catechol O-methyltransferase inhibitors, nitecapone and entacapone, in rat and mouse urine and rat bile

A LabVIEW®-based software for the control of the AUTORAD platform: a fully automated multisequential flow injection analysis Lab-on-Valve (MSFIA-LOV) system for radiochemical analysis

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