Radiobiological effect of Heparin in Swiss mice, Human Amnion cells andE. coli B/r irradiated with Co60 ?-rays

Chaubal, K.A.; Godbole, C. S.

doi:10.1007/bf01323678

Cited by 10 publications

(5 citation statements)

References 10 publications

(9 reference statements)

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“…Our finding on radioprotective efficacy of heparin agrees with those of other authors (Argutinskaya and Salganik 1963;Brückner 1976;Chaubal and Codbole 1983), supplement them and increase the sum of knowledge of the mechanism of action of this substance.…”

Section: Discussionsupporting

confidence: 92%

Radioprotective Effect of Heparin

Lukashin¹,

Grebenyuk²,

Zatsepin³

2011

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The study was aimed at the experimental assessment of the efficacy of heparin as radioprotector in condition of acute whole body γ-ray irradiation. The efficacy of the product was based on the 30-day survival rate and average life expectancy of dead irradiated animals. To reveal possible mechanisms of radioprotective effects of heparin the thymus mass, the number of leukocytes and colony formation units (CFU) in the peripheral blood and bone marrow were studied. It was shown that radioprotective effect of heparin administered intraperitoneally in a dose of 250 unit/kg was developed within one day after the administration and maintained for periods up to 30 days. Heparin increased the survival rate only in radiosensitive BALB/c and inbred mice. The product exerted stimulating effects on the migration of haemopoietic cells: injection of heparin doubled the number of CFU in the peripheral blood of BALB/c mice and reliably reduced it in the bone marrow.

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Section: Discussionsupporting

confidence: 92%

Radioprotective Effect of Heparin

Lukashin¹,

Grebenyuk²,

Zatsepin³

2011

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“…Several observations suggest a possible role for such molecules in protecting tissue from radical damage. Heparin alleviated the effects in vivo of radiations known to lead to free-radical production (Chaubel & Godbole, 1983), and heparin enhanced the activity in vivo of a superoxide dismutase (Flick et al, 1983). Intravenous injection ofheparin in man and some other mammals led to a prompt release of extracellular superoxide dismutase subtype C into the plasma (Karlsson & Marklund, 1987, 1988a.…”

Section: Introductionmentioning

confidence: 99%

Inhibition by heparin of Fe(II)-catalysed free-radical peroxidation of linolenic acid

Ross

Long

Williamson

1992

Biochemical Journal

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Heparin, in a concentration-dependent manner, inhibited the generation of conjugated dienes and thiobarbituric acid-positive substances when incubated with Fe2+ and gamma-linolenic acid. In the conjugated diene assay, other glycosaminoglycans, on a molar basis calculated with respect to their respective hydrated disaccharide repeat units, were less effective than heparin. Heparin which had been re-N-sulphonated after removal of both N-sulphonates and O-sulphates, and heparin in which iduronate residues had been reduced to idose residues, were largely unaffected in their activity. Removal of both N-sulphonates and O-sulphates greatly reduced the effectiveness of the heparin. Analysis of the effects of heparin fragments generated by heparinase I treatment of heparin showed that depolymerization decreased the effectiveness of the heparin. It is possible that heparins and related strongly acidic polysaccharides may function as endogenous antioxidants, and that sequestration by them, or harmless oxidation by them, of ions such as Fe2+, contributes to their effectiveness.

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“…Experimental information consistent with the above suggestion includes: the inhibition by a heparin of luminol-dependent chemiluminescence by neutrophils stimulated with serumactivated zymosan (Brestel & McClain, 1983); alleviation by heparin of the effects of radiations that are known to lead to free radical production (Chaubel & Godbole, 1983); production of a relatively stable heparin free radical species by radiation (Khan, 1982); and an inhibition of the Cu*+-catalysed oxidation of linoleic acid and of the decomposition of linoleate hydroperoxide to secondary oxidation products by particular carrageenans, which are algal extracellular sulphated polysaccharides, broadly similar in structure to heparins (Allen et al, 1982). In addition, heparin enhances the effect of administered exogenous superoxide dismutase in vivo (Flick et al, 1983), and induces the release into plasma of superoxide dismutase from endothelial cell surfaces (Karlsson & Marklund, 1987).…”

mentioning

confidence: 62%