Radioautographic localization of125I-atrial natriuretic fator (ANF) in rat tissues

Abstract

Rats were injected either with synthetic 125I-Arg 101-Tyr 126 atrial natriuretic factor (ANF) or with 125I-ANF together with an excess of cold Arg 101-Tyr 126 ANF. Binding sites in various tissues were accepted depending on two criteria: displacement of radioactivity by cold ANF and absence of localization of silver grains on putative target cells in the presence of cold ANF. Binding sites were localized on zona glomerulosa cells and on adrenergic and noradrenergic cells of adrenal medulla, on hepatocytes, on the base of mature epithelial cells of villi in the small intestine, on smooth muscle cells of the muscularis layer of the colon and on the base of epithelial cells of the ciliary bodies. In addition, binding sites were localized in the vasculature of kidney, adrenal cortex, lung and liver. Binding sites were particularly numerous on renal glomerular endothelial cells. These results indicate that ANF may have important hemodynamic effects in kidney, lung, liver and adrenal cortex, may regulate water and ion transport in small intestine and ciliary bodies and may have metabolic effects in the liver. The presence of binding sites on the zona glomerulosa is in agreement with the important inhibitory effect of the peptide on aldosterone secretion.

Relaxant effect of brain natriuretic peptide in nonsensitized and passively sensitized isolated human bronchi

“…Autoradiography has identified ANP in the Type II alveolar epithelial cells of rat lung, and NPR-A is expressed in various tissues including lung [7][8][9], suggesting the possibility that lung tissue is a target organ. In effect, ANP relaxes guinea pig [10,11], rat [10], bovine [12] and human [13] airway smooth muscle in vitro.…”

Marked and Prompt Hemodynamic Improvement by Carperitide in Refractory Congestive Heart Failure due to Dilated Cardiomyopathy.

“…However, several possible mechanisms may be considered. First, since atrial natriuretic hormone receptors are abundant in pulmonary blood vessels, 10) carperitide is thought to exert a potent pulmonary vasodilator effect, 1) and thus to reduce preload. Second, the natriuretic peptide has been reported to reduce the isovolumetric relaxation time of the LV, suggesting that this peptide may enhance LV diastolic relaxation 11,12) and thus reduce the preload.…”

Atrial natriuretic peptide (ANP) in rat gastrointestinal tract

“…Intravenously given radioactive ANP binds specifically, among other tissues, to the epithelial cells of the villi of the small intestine [10]. ANP inhibits Na-K-C1 cotransport across the intestinal wall in the flounder [12].…”

“…Radioactive ANP, when given intravenously in rats, is specifically bound by several tissues, including the mucosal layer of the small intestine, indicating the presence of ANP receptors in the tissue [10]. ANP given intracerebroventricularly has been found to stimulate gastric acid secretion I11].…”