Radioautographic Investigation of Serotonin Cells

Calas, A.; Dupuy, Jean-Jacques; Gamrani, Halima; Gonella, J; Mourre, Christiane; Condamin, Madeleine; Pellissier, Jean‐François; Bosch, Philippe Van den

doi:10.1007/978-1-4684-3860-4_3

Cited by 4 publications

(1 citation statement)

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“…At the same time, an enigmatic presence of 5-HT within the cell nuclei has been registered in a number of early works using electron microscopy and autoradiography techniques. Moreover, application of the radioactively marked 5-HT or its biochemical precursor 5-hydroxytryptophan (5-HTP) revealed strong localization of the signal within the cell nuclei, perinuclear space or even in condensed chromosomes. − Later, the immunochemical techniques also demonstrated the presence of 5-HT-immunoreactivity in adult neuronal and immune cell nuclei. These observations were confirmed by both fluorescent immunohistochemistry (IHC) and immunogold IHC combined with electron microscopy − as well as by direct identification of histone serotonylation in a recent study …”

Section: Introductionmentioning

confidence: 99%

Transglutaminase Activity Determines Nuclear Localization of Serotonin Immunoreactivity in the Early Embryos of Invertebrates and Vertebrates

Ivashkin

Melnikova

Kurtova

et al. 2019

ACS Chem. Neurosci.

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Serotonin (5-HT) is a key player in many physiological processes in both the adult organism and developing embryo. One of the mechanisms for 5-HT-mediated effects is covalent binding of 5-HT to the target proteins catalyzed by transglutaminases (serotonylation). Despite the implication in a variety of physiological processes, the involvement of serotonylation in embryonic development remains unclear. Here we tested the hypothesis that 5-HT serves as a substrate for transglutaminase-mediated transamidation of the nuclear proteins in the early embryos of both vertebrates and invertebrates. For this, we demonstrated that the level of serotonin immunoreactivity (5-HT-ir) in cell nuclei increases upon the elevation of 5-HT concentration in embryos of sea urchins, mollusks, and teleost fish. Consistently, pharmacological inhibition of transglutaminase activity resulted in the reduction of both brightness and nuclear localization of anti-5-HT staining. We identified specific and bright 5-HT-ir within nuclei attributed to a subset of different cell types: ectodermal and endodermal, macro-and micromeres, and blastoderm. Western blot and dot blot confirmed the presence of 5-HT-ir epitopes in the normal embryos of all the species examined. The experimental elevation of 5-HT level led to the enhancement of 5-HT-ir-related signal on blots in a species-specific manner. The obtained results demonstrate that 5-HT is involved in transglutaminase-dependent monoaminylation of nuclear proteins and suggest nuclear serotonylation as a possible regulatory mechanism during early embryonic development. The results reveal that this pathway is conserved in the development of both vertebrates and invertebrates.

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