Radioautographic analysis of <sup>3</sup>H‐fucose utilization by fibroblasts of the periodontal ligament

Cho, Moon‐Il; Garant, Philias R.

doi:10.1111/j.1600-0765.1986.tb01439.x

Cited by 3 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Free 'H-fucose, labeled metabolie glyeoprotein intermediates, and procollagen are readily soluble and are assumed to be washed frotn tissues during fixation and processing (Bennett, Leblotid & Haddad 1974), Keratan sulfate is not a major component of the rat transseptal ligament (Johnson 1988). Cho & Garant (1986) describe the incorporation of 'H-fucose into giycoproteins of the periodontal ligament by fibroblasts. It is probable that fibroblasts of the periodontal and transseptal ligaments utilize 'H-fucose similarly.…”

Section: Discussionmentioning

confidence: 99%

“…It is probable that fibroblasts of the periodontal and transseptal ligaments utilize 'H-fucose similarly. The Golgi becomes labeled within 5-10 min of isotope injection, secretion granules within 20 min, and extracellular matrix within 35 min (Cho & Garant 1986). In the present study, significant tissue labeling was observed 1 h after isotope injection, confirming the observations of Cho & Garant (1986).…”

Section: Discussionmentioning

confidence: 99%

“…The Golgi becomes labeled within 5-10 min of isotope injection, secretion granules within 20 min, and extracellular matrix within 35 min (Cho & Garant 1986). In the present study, significant tissue labeling was observed 1 h after isotope injection, confirming the observations of Cho & Garant (1986). In electron microscopic radioautographic preparations, silver grains are uniformly distributed within the extracellular matrix and are also associated with the cell surface (Cho & Garant 1986).…”

Section: Discussionmentioning

confidence: 99%

“…Anderson & Jackson (1972) and Anderson (1976) reported isolation of a fucosecontaining glycoprotein from bovine achilles tendon which interacts strongly with acid-soluble tropocoUagen, suggesting a role in macromoleeular organization and stabilization of collagen fibrils. Because the periodontal ligament incorporates 'H-fucose, this structural glycoprotein may also be a component of the periodontium (Cho & Garant 1986).…”

Section: Introductionmentioning

confidence: 99%

“…Gingival tissues contain an array of non-collagen ous glyeoproteins, but there is little information concerning their distribution or metabolism. Cho & Garant (1986) describe the synthetic pathway of ^H-fucose-labeled glycoproteins in periodontal ligament fibroblasts and report that 'H-fucose is 1) directly incorporated into proteins in the Golgi cisternae and saccules, 2) demonstrable within the same secretion granules as procollagen precursors, 3) secreted simultaneously with collagenous proteins by exocytosis, the product becoming incorporated into the extracellular matrix, and 4) uniformly distributed across the periodontal hgament in autoradiographic preparations. However, they neither quantify their results nor describe the transseptal ligament.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Distribution of ³H‐fucose in the transseptal ligament of the mouse

Johnson

1988

J of Periodontal Research

View full text Add to dashboard Cite

Johnson RB.: Distribution of^H-fucose in the transseptal ligament of the mouse. J Periodont Res 1988; 23: 363-369. The present study demonstrated 'H-fucose distribution in three regions of the transseptal hgament; the middle, mesial (adjaeent to the first molar tooth) and distal (adjacent to the second molar tooth) thirds; of 6-week-and 6-month-old Swiss mice. Incorporation of the isotope was rapid and maximal peaks occurred at 3 hours in most regions of 6-month and at 12 h in most regions of 6-wk animals: highest grain counts were over the middle third of the ligament of 6-wk animals (p< 0.001). During incorporation, mean grain counts were significantly different as a function of age (p<0.001), region (p<0.01) and time (p<0.001) and there was a significant interaction between region and time (p< 0.005). Grain removal occurred at a slower rate: there were significant differences in mean grain counts as a function of age (p<0.001), region (p<0.001) and time (p<0.00!) and a significant interaction between time and region (p<0.001) and between time, region and age (p<0.001). The highest rate of grain removal was in the middle third of the ligament of 6-wk animals. Half-hfe of labeled glycoproteins was shortest in the ligament of 6-wk animals. The study suggested regional differences in metabolism of fucose-containing glycoproteins of the transseptai ligament: highest turnover rates in the 1) middle third, and 2) young animals.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Distribution of ³H‐fucose in the transseptal ligament of the mouse

Johnson

1988

J of Periodontal Research

View full text Add to dashboard Cite

show abstract

³H‐mannose utilization by fibroblasts of the periodontal ligament

Cho¹,

Garant²

1987

Anat. Rec.

Self Cite

View full text Add to dashboard Cite

The synthesis, intracellular translocation, and secretion of mannose-containing glycoproteins(s) by periodontal ligament fibroblasts have been investigated by means of electron microscopic radioautography. Tritiated mannose was administered to young mice via jugular vein, and radioautographs were prepared at 5, 10, 20, and 35 minutes, 4 and 8 hours after injection. Analysis of electron microscopic radioautographs revealed a maximum labeling (94%) with 3H-mannose of the rough endoplasmic reticulum at 5 minutes. Labeling of the Golgi components started to increase from 10 minutes (14%) and reached a maximum level at 20 minutes (31.2%). At 35 minutes, secretion granules, dense bodies, profiles of intracellular collagen, and the cell surface were labeled. At 8 hours, most labelling (79.2%) was extracellular, and associated either with the collagenous matrix (43.7%) or the cell surface (35.5%). Cytoplasmic vesicles containing dense materials around collagen fibrils were also labeled at 8 hours. It is concluded that mannose is directly incorporated into the rough endoplasmic reticulum (RER), and that mannose-containing glycoprotein(s) are packaged in the Golgi apparatus into secretory granules. Mannose-containing glycoprotein(s) become distributed on the periodontal ligament (PDL) fibroblast cell surface, cytoplasmic dense bodies, and the extracellular matrix.

show abstract

Traffic through the golgi apparatus as studied by radioautography

Bennett

Wild

1991

J. Elec. Microsc. Tech.

View full text Add to dashboard Cite

The ability to radiolabel biological molecules, in conjunction with radioautographic or cell fractionation techniques, has brought about a revolution in our knowledge of dynamic cellular processes. This has been particularly true since the 1940's, when isotopes such as 35S and 14C became available, since these isotopes could be incorporated into a great variety of biologically important compounds. The first dynamic evidence for Golgi apparatus involvement in biosynthesis came from light microscope radioautographic studies by Jennings and Florey in the 1950's, in which label was localized to the supranuclear Golgi region of goblet cells soon after injection of 35S-sulfate. When the low energy isotope tritium became available, and when radioautography could be extended to the electron microscope level, a great improvement in spatial resolution was achieved. Studies using 3H-amino acids revealed that proteins were synthesized in the rough endoplasmic reticulum, migrated to the Golgi apparatus, and thence to secretion granules, lysosomes, or the plasma membrane. The work of Neutra and Leblond in the 1960's using 3H-glucose provided dramatic evidence that the Golgi apparatus was involved in glycosylation. Work with 3H-mannose (a core sugar in N-linked side chains), showed that this sugar was incorporated into glycoproteins in the rough endoplasmic reticulum, providing the first radioautographic evidence that glycosylation of proteins did not occur solely in the Golgi apparatus. Studies with the tritiated precursors of fucose, galactose, and sialic acid, on the other hand, showed that these terminal sugars are mainly added in the Golgi apparatus. With its limited spatial resolution, radioautography cannot discriminate between label in adjacent Golgi saccules. Nonetheless, in some cell types, radioautographic evidence (along with cytochemical and cell fractionation data) has indicated that the Golgi is subcompartmentalized in terms of glycosylation, with galactose and sialic acid being added to glycoproteins only within the trans-Golgi compartment. In the last ten years, radioautographic tracing of radioiodinated plasma membrane molecules has indicated a substantial recycling of such molecules to the Golgi apparatus.

show abstract

Radioautographic analysis of ³H‐fucose utilization by fibroblasts of the periodontal ligament

Cited by 3 publications

References 23 publications

Distribution of ³H‐fucose in the transseptal ligament of the mouse

Distribution of ³H‐fucose in the transseptal ligament of the mouse

³H‐mannose utilization by fibroblasts of the periodontal ligament

Traffic through the golgi apparatus as studied by radioautography

Contact Info

Product

Resources

About

Radioautographic analysis of 3H‐fucose utilization by fibroblasts of the periodontal ligament

Cited by 3 publications

References 23 publications

Distribution of 3H‐fucose in the transseptal ligament of the mouse

Distribution of 3H‐fucose in the transseptal ligament of the mouse

3H‐mannose utilization by fibroblasts of the periodontal ligament

Traffic through the golgi apparatus as studied by radioautography

Contact Info

Product

Resources

About

Radioautographic analysis of ³H‐fucose utilization by fibroblasts of the periodontal ligament

Distribution of ³H‐fucose in the transseptal ligament of the mouse

Distribution of ³H‐fucose in the transseptal ligament of the mouse

³H‐mannose utilization by fibroblasts of the periodontal ligament