Radio-thermo-sensitivity Induced by Gold Magnetic Nanoparticles in the Monolayer Culture of Human Prostate Carcinoma Cell Line DU145

Abstract

Background and Objective: Prostate cancer is the second cause of death in men worldwide. In this study, the cytotoxic effects of PLGA polymer-coated gold Magnetic Nanoparticles (MGNPs), as a novel treatment to enhance radiation and thermal sensitivity in the presence of hyperthermia (43°C) and electron beam, on DU145 prostate cancer cells were investigated. Methods: Nanoparticles were characterized using TEM, DLS, XRD and SAED methods. MGNPs entrance into the cells was determined using Prussian blue staining and TEM. Furthermore, the cytotoxic effects of combinatorial treatment modalities were assessed by applying colony and sphere formation assay. Results: Our results revealed that the decrease of colony and sphere numbers after combinatorial treatment of hyperthermia and radiation in the presence of nanoparticles was significantly higher than the other treatment groups (P<0.05). This treatment method proved that it has the capability of eliminating most of the DU145 cells (80-100%), and increased the value of the linear parameter (α) to 4.86 times. Conclusion: According to the study, magnetic gold nanoparticles, in addition to having a high atomic number, can effectively transmit heat produced inside them to the adjacent regions under hyperthermia, which increases the effects of radio-thermosensitivity, respectively.

Emerging importance of nanotechnology-based approaches to control the COVID-19 pandemic; focus on nanomedicine iterance in diagnosis and treatment of COVID-19 patients

“…Otechnology in medicine finds importance in several overlapping molecular technologies [ 133 , [135] , [136] , [137] , [138] ].…”

Gold Nanoparticle Effects on the Hounsfield Unit of Computed Tomography in Hepatocellular Carcinoma: Systematic Review

“…Scientists have sought to use nanoparticles (NPs) as imaging contrast agents owing to their unique physical and chemical characteristics, great biocompatibility, considerable stability and small dimensions [60][61][62][63] . According to ndings, gold nanoparticles (AuNPs) possess characteristics such as plasmonic nature, non-toxicity, surface-level change, high X-ray absorption due to high atomic number [64][65][66][67][68][69][70][71][72][73][74][75] , and prolonged blood circulation time 3 . These properties has distinguished them as interesting candidates for application as contrast agents 76,77 .…”