Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark

Lawrence, Lincy; Menon, Seema; Vincent, S G Prakash; Sivaram, V.; Padikkala, José

doi:10.1016/j.jaim.2016.06.003

Cited by 20 publications

(16 citation statements)

References 19 publications

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“…Neutrophils are recruited to the site of injury lead to ROS production that results in oxidative stress to mucosal cells and ulcer. Therefore, the use of antioxidants might be helpful in protecting the mucosal cells from oxidative damage and speeding up the healing process [12]. The constituents of Lavandula stoechas L. include camphor, linalool, linalyl acetate, 1-8 cineole and γterpinene while its antioxidant potential is found to be comparable with vitamin E as reported in previous studies [13].…”

Section: Introductionsupporting

confidence: 58%

“…The exact mechanism how ethanol causes gastric lesions is not fully understood [9, 10] however, ethanol is reported to produce a disturbance in gastric mucosal barrier via exfoliation of cells [11,35] followed by the recruitment of inflammatory cells to the site of injury and production of reactive oxygen species (ROS). As ROS and other mediators of inflammation may lead to oxidative damage [12,36] therefore, by scavenging free radicals, antioxidants might prove as useful gastroprotective agents. In the present study, both the aqueous and methanolic Lavandula stoechas L. extracts showed strong free radical scavenging activity as determined by DPPH method (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Oral administration of ethanol to experimental animals induced gastrointestinal lesions [9,10]. The process of ethanol-induced injury is not fully known however; it causes a disturbance in gastric mucosal integrity through exfoliation of cells and thus, may lead to gastric mucosal bleeding [11,12]. Neutrophils are recruited to the site of injury lead to ROS production that results in oxidative stress to mucosal cells and ulcer.…”

Section: Introductionmentioning

confidence: 99%

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Pharmacological Evaluation of Lavandula stoechas L. for Ethanol-induced Gastric Mucosal Ulcer

Javed

Khan

Arshad³

et al. 2020

RADS J. Pharm. Pharm. Sci.

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Background: The constituents of Lavandula stoechas L. possess antioxidant properties that help in protecting the mucosal cells from oxidative damage and speed up the healing process however, its role in the treatment of ethanol-induced peptic ulcers is not clear. Objectives: We aimed to evaluate the pharmacological potential of Lavandula stoechas L. extracts for anti-ulcer activity, and compare with the standard drugs and to explore novel treatment for peptic ulcer. Methods: We evaluated anti-ulcer potential of plant extract in ethanol-induced ulcer model in rats. Omeprazole and ranitidine were standard drugs. After 5 h of disease induction, animals were sacrificed to get tissues for histological evaluation and ulcer index was measured. While the antimicrobial potential of Lavandula stoechas L. aqueous and methanolic extracts was evaluated against different bacterial stains using standard antibiotic discs. Qualitative phytochemical and GCMS analysis were performed to identify novel constituents. Results: The methanolic extract of Lavandula stoechas L. showed antimicrobial activity against Proteus Mirabilis while the GCMS based analysis revealed the presence of 10 phytochemicals including camphor (antimicrobial agent). The aqueous extract showed significant anti-ulcer activity in ethanol-induced gastric (P < 0.001) and duodenal (P < 0.01) ulcers when compared with controls. Aqueous and methanolic Lavandula stoechas L. extracts showed strong free radical scavenging activity. Conclusion: Lavandula stoechas L. extract possess antimicrobial and anti-ulcer activity in alcohol-induced ulcer model in experimental animals.

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Section: Introductionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pharmacological Evaluation of Lavandula stoechas L. for Ethanol-induced Gastric Mucosal Ulcer

Javed

Khan

Arshad³

et al. 2020

RADS J. Pharm. Pharm. Sci.

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“…In addition, reduction in MDA levels were reported in the treated rats with several compounds, such as 3-(2-Chlorophenyl)-1-phenyl-propenone and Zn(II)–curcumin complex [51, 52]. It is reported that ROS, such as hydroxyl radicals, superoxide anions, and lipid peroxides are harmful species which is known to provoke gastric ulcer development [53]. Free radical ultimately causes depletion in tissue antioxidant status leading to lipid peroxidation.…”

Section: Discussionmentioning

confidence: 99%

Gastroprotective activity of a novel Schiff base derived dibromo substituted compound against ethanol-induced acute gastric lesions in rats

Saremi

Rad

Tayeby

et al. 2019

BMC Pharmacol Toxicol

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Background Basic function of bromine in body is to activate pepsin production in gastritis with low acidity. The present study encompasses a broad in vivo study to evaluate gastroprotective activity of a novel dibromo substituted Schiff base complex against Sprague Dawley (SD) rats. Methods 2, 2′-[1, 2-cyclohexanediylbis (nitriloethylidyne)]bis(4-bromophenol) (CNBP) is synthesized via a Schiff base reaction, using the related ketone and diamine as the starting materials. SD rats are divided as normal, ulcer control (5 ml/kg of 10% Tween 20), testing (10 and 20 mg/kg of CNBP) and reference groups (omeprazole 20 mg/kg). Except for the normal group, the rest of the groups are induced gastric ulcer by ethanol 1 h after the pre-treatment. Ulcer area, gastric wall mucus, and acidity of gastric content of the animal stomachs are measured after euthanization. Antioxidant activity of the compound is tested by Ferric reducing antioxidant power (FRAP) test and safety of the compound is identified through acute toxicity by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Moreover, activities of superoxide dismutase (SOD), catalase (CAT), levels of prostaglandins E 2 (PGE 2 ) and also malondialdehyde (MDA) are determined. Results Antioxidant activity of CNBP was approved via FRAP assay. Vast shallow hemorrhagic injury of gastric glandular mucosa was observed in the ulcer group compared to the CNBP-treated animals. Histological evaluations confirmed stomach epithelial defense effect of CNBP with drastic decrease of gastric ulceration, edema and leucocytes penetration of submucosal stratum. Immunostaining exhibited over-expression in HSP70 protein in CNBP-treated groups compared to that of the ulcer group. Also, gastric protein analysis showed low levels of MDA, PGE 2 and high activity of SOD and CAT. Conclusions CNBP with noticeable antioxidant property showed gastroprotective activity in the testing rodents via alteration of HSP70 protein expression. Also, antioxidant enzyme activities which were changed after treatment with CNBP in the animals could be elucidated as its gastroprotective properties.

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“…The anti-inflammatory effect of methanol extract of G. arborea and its fractions can be attributed to the presence of the flavonoid [8] present therein. Moreover, the insecticidal activity of G. arborea extracts against Megalurothrips sjostedti [9] and Callosobruchus chinensis and Sitophilus oryzae [10], would healing effects [11], anti-ulcer potentials [12] and antiangiogenic activity [13] have been reported. It was also reported that an ethanolic leaf extract of G. arborea has potent in vitro cytotoxic activity against colon cancer (COLO 201), gastric cancer (HT-29) and human oesophagel cancer (TE-2) cell lines [14].…”

Section: Introductionmentioning

confidence: 99%

Anti-nociceptive and Anti-inflammatory Effects of Gmelina arborea Roxb. e x. Sm.

Adebayo¹

2018

Invest Med Chem Pharmacol

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Background: The aim of the present study was to evaluate the anti-inflammatory and anti-nociceptive activities of the methanolic extract of Gmelina arborea fruit (GAF) and stem bark (GASB) on egg-albumin induced inflammation and heated plate metal respectively. Methods: 500 g of each of the pulverised samples was extracted with 90% methanol using soxhlet extractor to produce dense mass of methanolic extracts after distillation and kept in a refrigerator at 4 o C until further analysis. The anti-inflammatory activity was determined on fresh egg albumins over 4 h by measurement of rat paw oedema according to established procedure. The anti-nociceptive was evaluated by placing each rat on the heated metal plate (Hot plate) maintained at the temperature of about 50-55 0 C within the restraining plastic cylinder while animals responses were taken over 90 min. Results: Methanolic extracts of G. arborea yielded blackish thick mass and greenish paste viscous extract for the GASB and GAF respectively with percentage yields of 28% and 30% respectively. The yield revealed more extractives in the GAF than GASB. The anti-nociceptive properties of both GAF and GASB shows a relatively time dependent activity as rate of inhibition was high at the 30 th min (at the peak of p<0.001) for all doses except for 100 mg/kg per oral for the GAF. At increased time, the activity of most of the extracts reduced considerably. The eggalbumin induced inflammation was inhibited by the extracts as well in different doses. There were considerable inhibition for the GAF as well as the GASB at a minimum significant value of p<0.05 from the 1 st h to the 4 th h with maximum percentage inhibition (PI) values ranging between 30% to a peak of 70% when compared to the control. The methanolic extract of G. arborea fruit and stem bark showed significant (p<0.05) anti-inflammatory activity. Conclusions: This study has shown that GASB and GAF displayed significant anti-inflammatory effect on paw oedema induced by egg-albumin by inhibiting the release of mediators for the entire 4 h experimental period. The anti-nociceptive actions were also significant when compared with the control.

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Radical scavenging and gastroprotective activity of methanolic extract of Gmelina arborea stem bark

Cited by 20 publications

References 19 publications

Pharmacological Evaluation of Lavandula stoechas L. for Ethanol-induced Gastric Mucosal Ulcer

Pharmacological Evaluation of Lavandula stoechas L. for Ethanol-induced Gastric Mucosal Ulcer

Gastroprotective activity of a novel Schiff base derived dibromo substituted compound against ethanol-induced acute gastric lesions in rats

Anti-nociceptive and Anti-inflammatory Effects of Gmelina arborea Roxb. e x. Sm.

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