Radical scavenging and angiotensin converting enzyme inhibitory activities of standardized extracts of Ficus racemosa stem bark

Abstract

The present study evaluated the radical scavenging and angiotensin converting enzyme (ACE) inhibitory activity of cold and hot aqueous extracts of Ficus racemosa (Moraceae) stem bark. The extracts were standardized using HPLC. Radical scavenging activity was determined using 1,1-diphenyl-2-picrylhydrazyl radical and angiotensin converting enzyme inhibitory activity using rabbit lung and partially purified porcine kidney ACE. HPLC profiles of cold aqueous extract (FRC) showed the presence of bergenin, an isocoumarin, while hot aqueous extract (FRH) was found to contain ferulic acid, kaempferol and coumarin in addition to bergenin. FRH showed significantly higher (p ≤ 0.01) radical scavenging activity than FRC and butylated hydroxytoluene (BHT), consequently resulting in a significantly lower (p ≤ 0.01) IC₅₀ value than FRC and BHT. Both the extracts exhibited a dose dependent inhibition of porcine kidney and rabbit lung ACE. FRH showed significantly higher (p ≤ 0.01) activity than FRC with lower IC(50) values of 1.36 and 1.91 μg/mL respectively, for porcine kidney and rabbit lung ACE, compared with those of FRC (128 and 291 μg/mL). Further, a significant correlation (r = 0.893; p ≤ 0.05) was observed between radical scavenging activity and ACE-inhibitory activity. This is the first report on the ACE-inhibitory activity of F. racemosa stem bark suggesting its potential to be utilized as a therapeutic alternative for hypertension.

Extraction, optimisation and characterisation of phenolics from Thymus vulgaris L.: phenolic content and profiles in relation to antioxidant, antidiabetic and antihypertensive properties

“…Ahmed et al . () reported a strong positive correlation between antioxidant activity and ACE‐inhibitory activity in the stem bark of Ficus racemosa ( r = 0.893), whereas Nho et al . () reported negative correlations between the antioxidant activity and ACE‐inhibitory activity of the extracts in Boehmeria nivea and its leaves.…”

Thai herbal antipyretic 22 formula (APF22) inhibits UVA‐mediated melanogenesis through activation of Nrf2‐regulated antioxidant defense

“…(Sakpakdeejaroen, Juckmeta, & Itharat, ). Several phytochemicals identified in the component herbs of the APF22 included flavonoids and phenolic acid derivatives such as ferulic acid (FA), gallic acid (GA), hispidulin, and kaempferol (Ahmed, Siddesha, Urooj, & Vishwanath, ; Hazekamp, Verpoorte, & Panthong, ; Yadav, Nandy, Maity, Sarkar, & Saha, ). Previous in vitro and in vivo studies have demonstrated beneficial effects of the APF22 and its component herbs on skin disorders and wound healing (Juckmeta et al, ; Waheed et al, ; Murti & Kumar, ).…”

Protective effects of Ficus racemosa stem bark against doxorubucin-induced renal and testicular toxicity

“…[9] The bark has also shown to possess antidiabetic, antibacterial, anticholinesterase, acetylcholine enhancing and angiotensin converting enzyme inhibitory activities. [10–13] We have also reported F. racemosa bark extract to exhibit potential hepatoprotective activity against CCl 4 -induced hepatotoxicity and cardioprotective effect against doxorubucin-induced cardiotoxicity in rats. [1415] In view of this, the present study evaluated the protective effects of standardized extract of F. racemosa stem bark against doxorubicin-induced renal and testicular toxicity in albino rats.…”