Radical Scavenging Activities of Tannin Extracted from Amaranth (Amaranthus caudatus L.)

Abstract

This study investigates the bioactivity of tannin from amaranth (Amaranthus caudatus L.) extracts. The antioxidant activities of the extracts from amaranth leaves, flowers, and seeds were evaluated. Tannin from leaves of amaranth has been evaluated for superoxide scavenging activity by using DPPH and ABTS(+) analysis, reducing power, protective effect against H2O2-induced oxidative damage in L-132 and BNL-CL2 cells, and inhibition of superoxide radical effects on HL-60 cells. At a concentration of 100 μg/ml, tannin showed protective effects and restored cell survival to 69.2% and 41.8% for L-132 and BNL-CL2 cells, respectively. Furthermore, at the same concentration, tannin inhibited 41% of the activity of the superoxide radical on HL-60 cells and 43.4% of the increase in nitric oxide levels in RAW 264.7 cells. The expression levels of the antioxidant-associated protein SOD-1 were significantly increased in a concentration-dependent manner in RAW 264.7 cells treated with tannin from amaranth leaves. These results suggest that tannin from the leaves of Amaranthus caudatus L. is a promising source of antioxidant component that can be used as a food preservative or nutraceutical.

Antioxidant and phytochemical activities of Amaranthus caudatus L. harvested from different soils at various growth stages

“…Previously, Jo et al . 43 and Enujiugha et al . 41 respectively had reported lower values of proanthocyanidin (0.516 ± 0.02 and 0.045 ± 0.000 mg TAE/g) in A. caudatus using the tannic acid standard as opposed catechin standard used in this study.…”

Aloe vera and Virgin Coconut Oil (VCO) accelerate healing process in domestic cat (Felis domesticus) suffering from scabies

“…Tannins function as an astringent that can cause narrowing of the pores of the skin, harden the skin and act as an antimicrobial and antifungal so as to prevent secondary infection in the wound. Tannins are polyphenols with strong antioxidant activity that can protect against damage to free radicals caused by UV exposure and reduce the risk of skin cancer (25). Tannins are able to reduce the production of H2O2, inhibits the induction of ornitine decarboxylase and stimulates DNA synthesis in the epidermis (26).…”

Phytochemical, cytotoxic, and genotoxic evaluation of protein extract of Amaranthus hypochondriacus seeds

“…Amaranth grain has the potential to contribute to improving the nutrition of populations, especially in developing countries, because it has nutritional and functional properties (Muyonga et al, 2008). The presence of some compounds has been reported, such as phenols, fatty acids, tocopherols, carotenoids and others that contribute to health, reducing the risk of oxidative stress related to chronic diseases (Jo et al, 2015;López et al, 2013;Tang & Tsao, 2017). However, several researchers have demonstrated that amaranth seeds may contain antinutritional compounds, which could affect their nutritional value and limit their use.…”