Radiationless decay of singlet molecular oxygen in solution. Experimental and theoretical study of electronic-to-vibrational energy transfer

Merkel, Paul B.; Kearns, David R.

doi:10.1021/ja00776a003

Cited by 578 publications

(267 citation statements)

References 3 publications

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“…In control experiments, neither enzyme inhibited significantly when inactivated by autoclaving, and 100 ,ug each of two other proteins, bovine serum albumin and lysozyme, had no effect on activity. (23)(24)(25)(26), either stimulated chemiluminescence or had no effect on activity. We examined the action of these agents on the chemiluminescence of the xanthine oxidase-acetaldehyde system, which is mediated by oxygen radicals (19 (13,31), which has been attributed to removal of the direct oxidative effects of O2 (31).…”

Section: Resultsmentioning

confidence: 96%

Generation of chemiluminescence by a particulate fraction isolated from human neutrophils. Analysis of molecular events.

McPhail¹,

DeChatelet²,

Johnston³

1979

J. Clin. Invest.

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A B S T R A C T A particulate fraction isolated from human neutrophils by homogenization, then centrifugation at 27,000 g, was demonstrated to generate chemiluminescence. This luminescence required the addition of reduced pyridine nucleotide and was very low in fractions from resting normal cells. Stimulation of neutrophils with opsonized zymosan, phorbol myristate acetate, or ionophore A23187 resulted in marked enhancement of the chemiluminescence measured in subsequently isolated particulate fractions. Stimulation did not boost the luminescence produced by fractions from cells of patients with chronic granulomatous disease. The chemiluminescence of particulate fractions from stimulated neutrophils was linear with increasing protein concentration, had a pH optimum of 7.0, and was higher with NADPH as substrate than with NADH. These results confirm previous studies suggesting that the enzyme system responsible for the respiratory burst in neutrophils is present in this fraction. The particulate fraction was used to examine the nature and origin of neutrophil luminescence by investigating the effect on this phenomenon of certain chemical and enzymatic scavengers of oxygen metabolites. Results suggest that the energy responsible for the luminescence of particulate fractions and, presumably, the intact cell, is derived from more than one oxygen species and that luminescence is a product of the interaction of these species and excitable substrates within the cell.

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Section: Resultsmentioning

confidence: 96%

Generation of chemiluminescence by a particulate fraction isolated from human neutrophils. Analysis of molecular events.

McPhail¹,

DeChatelet²,

Johnston³

1979

J. Clin. Invest.

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“…Hanks' solution with D20 was used as suspension medium in order to evaluate the role of singlet molecular oxygen (102). The lifetime of 102 is about 10 x longer in D20 than in H20 (Merkel & Kearns, 1972;Gorman & Rodgers, 1978) and a sensitizing effect of D20, such as that seen in Fig. 2, indicates that 102 is involved in the inactivation.…”

Section: Detection Of 102 By Esrmentioning

confidence: 86%

“…Their conclusion was based on the protective effect of 1,3-diphenylisobenzofuran against photoinactivation. 1,3-diphenylisobenzofuran is known as an efficient singlet-oxygen trap (Merkel & Kearns, 1972). However, this observation does not unambiguously rule out the "free radical" mechanism (Type I), since 1,3-diphenylisobenzofuran is also a good radical quencher (Lamola, 1976 In the following, we assume that the inactivation is mainly due to 102.…”

Section: Molecular Mechanism Of Inactivationmentioning

confidence: 99%

The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin

Moan¹,

Pettersen²,

Christensen³

1979

Br J Cancer

250

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Summary.-The photosensitizing effect of haematoporphyrin (HP) on human cells of the established line NHIK 3025 has been studied. Fluorescence measurements show that HP is bound to these cells. Serum proteins also bind HP, and the presence of 10% human serum during incubation with HP (3 x 10-4M) reduces the cellular uptake of HP by 75%o or more. The photosensitized inactivation is enhanced when the cells are suspended in D20-buffer during irradiation. This indicates that singlet oxygen is involved in the inactivation.Two findings indicate that the photoinduced damage is repairable: firstly, the fraction of cells surviving a given light dose decreases with decreasing irradiation temperature, and secondly, the survival curves have a shoulder at low exposures of light.

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“…The hydroxyl bonds in water molecules act as quenchers for singlet oxygen (Merkel and Kearns, 1972), so that in D 2 0 , which quenches less efficiently, the lifetime is significantly extended. The best estimate of singlet lifetime in DzO is about 55 ps (Rodgers and Snowden, 1982;Rodgers, 1983).…”

Section: Characteristics Of Singlet Oxygen In Membranesmentioning

confidence: 99%

Photomodification of Biological Membranes With Emphasis on Singlet Oxygen Mechanisms

Valenzeno

1987

Photochem & Photobiology

263

118

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Abstract-This review discusses photomodification of biological membranes and model membrane systems. Current concepts of membrane structure are first reviewed briefly. The role of preillumination association of sensitizer with membranes as it relates to photomodification rate is discussed, as well as the role of singlet oxygen in membrane photomodification. Finally the characteristics of singlet oxygen generation in membranes are considered. The evidence clearly indicates that membrane photomodification cannot be understood based only on the properties of sensitizers and singlet oxygen in aqueous solution. Rather the properties of sensitizers in association with membranes are the determinants of membrane photomodification. These properties differ significantly in aqueous solution and in membranes.

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Radiationless decay of singlet molecular oxygen in solution. Experimental and theoretical study of electronic-to-vibrational energy transfer

Cited by 578 publications

References 3 publications

Generation of chemiluminescence by a particulate fraction isolated from human neutrophils. Analysis of molecular events.

Generation of chemiluminescence by a particulate fraction isolated from human neutrophils. Analysis of molecular events.

The mechanism of photodynamic inactivation of human cells in vitro in the presence of haematoporphyrin

Photomodification of Biological Membranes With Emphasis on Singlet Oxygen Mechanisms

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