Radiation technology in the preparation of polyethylene oxide hydrophilic gels and Immobilization of Proteases for use in medical practice

Abstract

This paper deals with the development of a technology for making a hydrophilic gel of polyethylene oxide reception in which radiating ability is employed to cause cross-linking of polymers in a water solution. The gel of polyethylene oxide was shown to be non-toxic, contain 5-50% of polymer and be useful in composite medicinal forms along with biologically active substances including Bac. subtilis proteases. Proteases immobilized in the gel possess high thermal stability and proteolytic activity and are readily applied in medicine. The effect of immobilized proteolytic and glucolytic enzymes of Bac. subtillis (Immozimase) on the warm ischemia-reperfusion (I/R) which can cause hepatic and jejunum injury was also studied. These enzymes were immobilized on water-soluble polymer polyethylene glycol by means of an electron beam. The number of degranulated mast cells as well as serum ALT after I/R in the group with Immozimase was decreased to almost half as compared with the control group. Pretreatment with Immozimase resulted in significant reduction of hepatic and gut neutrophil accumulation as compared with control animals. It was concluded that Immozimase has a protective effect for hepatic and gut ischemia/reperfusion, and this effect seems to be associated with prevention of leukocyte accumulation.

Mobilization of Progenitor Cells into the Blood by Immobilized Granulocytic Colony-Stimulating Factor

“…Molecules of nonglycosylated G-CSF (Siberian Center of Pharmacology and Biotechnologies) were immobilized on low-molecular-weight polyethylenoxide by nanotechnology of radiation synthesis using directed beam of accelerated electrons [15].…”

Mechanisms of Granulocytopoiesis-Stimulating Activity of Immobilized Granulocytic Colony-Stimulating Factor in Cytostatic Myelosuppression

“…The molecules of G-CSF (Siberian Center of Pharmacology and Biotechnologies) were immobilized on low-molecular polyethylenglycol by the radiation synthesis nanotechnology using directed fl ow of accelerated electrons [13].…”

Antithrombotic and Thrombolytic Effects of a New Proteolytic Preparation Trombovazim (Russia)

“…Course treatment with Trombovazim (1000 U intragastrically, twice daily for 3 days) had a thrombolytic effect on rats with experimental intravascular thrombosis. This effect was manifested in a decrease in thrombus weight and increase in the percent of rats with recanalization of the occluded carotid artery.The development of nanomolecular drugs is directed to passage through the blood-tissue barriers, reduction of side effects (toxicity and allergic properties), and increase in the life-time of products in the body (prevention of enzymatic degradation and accumulation in the reticuloendothelial system; inhibition of renal filtration) [4,5,6]. A new preparation Trombovazim was developed at the Siberian Center of Pharmacology and Biotechnology.…”

“…The development of nanomolecular drugs is directed to passage through the blood-tissue barriers, reduction of side effects (toxicity and allergic properties), and increase in the life-time of products in the body (prevention of enzymatic degradation and accumulation in the reticuloendothelial system; inhibition of renal filtration) [4,5,6]. A new preparation Trombovazim was developed at the Siberian Center of Pharmacology and Biotechnology.…”

“…A new preparation Trombovazim was developed at the Siberian Center of Pharmacology and Biotechnology. A highly-purified enzyme preparation was obtained by immobilization of Bacillus proteinases on polyethylene oxide 1500 [1,6]. Trombovazim is manufactured by the method of electron-beam synthesis.…”