Radiation Synthesis of Selenium Nanoparticles Capped with β-Glucan and Its Immunostimulant Activity in Cytoxan-Induced Immunosuppressed Mice

Abstract

Selenium nanoparticles (SeNPs) with diameters from 64.8 to 110.1 nm were successfully synthesized by γ-irradiation of solutions containing Se4+ and water-soluble yeast β-glucan. The size and size distribution of SeNPs were analyzed by dynamic light scattering (DLS). Analytical X-ray diffraction (XRD) pattern results confirmed the crystal structure of the Se nanoparticles and Fourier transform infrared (FTIR) spectroscopy revealed that β-glucan could interact with SeNPs through steric (Se…O) linkages leading to a homogeneous and translucent solution state for 60 days without any precipitates. In vivo tests in cytoxan-induced immunosuppressed mice revealed that the daily supplementation of SeNPs/β-glucan at concentrations of 6 mg per kg body weight of tested mice significantly stimulated the generation of cellular immune factors (white blood cells, neutrophil, lymphocyte, B cells, CD4+ cells, CD34+ cells and natural killer cells) and humoral immune indexes (IgM, IgG, TNF-α, IFN-γ and IL-2) in peripheral blood, bone marrow and spleen of the immunosuppressed mice. The obtained results indicated that radiation-synthesized SeNPs/β-glucan may be a candidate for further evaluation as an agent for the prevention of immunosuppression in chemotherapy.

Mushroom-Derived Novel Selenium Nanocomposites’ Effects on Potato Plant Growth and Tuber Germination

“…Thus, the role of the biopolymeric envelope consisted in providing a sustained dispersion of SeNPs and giving rise to formation of nanostructures without their apparent clustering, enabling the consequent formation of bio-composites. The fact that the major extracellular polysaccharidic molecules should be responsible for reduction and subsequent stabilization during the formation of SeNPs, was observed with a number of polyglucans and inorganic Se-precursor compounds in the course of physicochemical processes; for example, γ-irradiation of solutions containing Se 4+ ions and water-soluble yeast β-glucan, resulted in synthesis of SeNPs [ 46 ]. Fourier transform infrared (FTIR) spectroscopy revealed that β-glucan could interact with SeNPs through linkages between selenium and the oxygen atoms that are abundant in polysaccharide, resulting in a homogeneous and semitransparent solution.…”

“…Fourier transform infrared (FTIR) spectroscopy revealed that β-glucan could interact with SeNPs through linkages between selenium and the oxygen atoms that are abundant in polysaccharide, resulting in a homogeneous and semitransparent solution. Chemically and phytochemically synthesized SeNPs, capped with polyglucans, were recently revealed to show an immunostimulant activity [ 46 , 47 ], and to display strong antibacterial and antifungal effects [ 48 , 49 ].…”

Glucose Hydrogenolysis into 1,2-Propanediol Using a Pt/deAl@Mg(OH)2 Catalyst: Expanding the Application of a Core–Shell Structured Catalyst

“…04-0802 library, indicating that the platinum nanoparticles are successfully loaded onto the catalyst. After loading Mg(OH) 2 , the half-peak width of Pt (111) and (200) in the catalyst increases, which confirms that the Mg(OH) 2 shell can effectively prevent the agglomeration of Pt nanoparticles under hydrothermal conditions and promote the dispersion of Pt nanoparticles [ 27 ]. There is no peak of Mg(OH) 2 in the XRD analysis, which suggests that Mg(OH) 2 covers the surface of the molecular sieve in an amorphous manner ( Figure S2 ).…”

Progress in the Surface Functionalization of Selenium Nanoparticles and Their Potential Application in Cancer Therapy

“…It was reported that the particle size of SeNPs/Oligochitosan (OCS) powder prepared by spray drying was slightly increased to 43.8 nm as compared to the SeNPs/OCS solution with an average size of 41.8 nm [ 76 ]. Particularly, the particle sizes of SeNPs/β-Glucan powder increased from 92 nm in the solution to 95, 117.6, and 132.3 nm prepared by freeze-drying, coagulation, and spray drying techniques, respectively, indicating that the freeze-drying method is the best way to preserve SeNPs [ 77 ].…”