Radiation-resistant <i>Macrococcus caseolyticus</i> (A) isolated from radiation-processed semidried prawns

Karani, M; Shashidhar, Ravindranath; Kakatkar, Aarti S.; Gautam, Raj Kamal; Sukhi, Shibani; Pansare-Godambe, Lipika; Bandekar, Jayant R.

doi:10.1139/cjm-2014-0636

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…EK1, EK2, EK4 and EK7 grown at between 2-4% NaCl while EK3, EK5 and EK6 could grow at between 4-10%, 4-6% and 4-8% NaCl, respectively. Karani et al 27 revealed that M. caseolyticus strain developed in the presence of salt as high as 8%. Diaz et al 15 stated that halophilic or halo-tolerance microorganisms tolerated high salt concentrations and this was making them very important in remediation.…”

Section: Resultsmentioning

confidence: 99%

“…Optimum pH range was 8.5-9 for A. urinaeequi 32 , 6-7 for S. salivarius 33 , 6 for L. sakei 34 and 9.6 for L. garvieae 35 . Karani et al 27 found that the optimum pH range for M. caseolyticus was 6-11. Dhakar and Pandey 36 emphasized that bacteria, archaea and eukaryotic organisms that had the potential to develop in a wide pH range could be used in industrially important bioprocesses.…”

Section: Resultsmentioning

confidence: 99%

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Bacteriocin Producing Bacteria Isolated from Turkish Traditional Sausage Samples

Erkaya¹,

Genç²,

Akbulut³

et al. 2020

J Pure Appl Microbiol

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In this study, traditional sausage samples from different provinces of Turkey (Gaziantep, Antalya, erzurum and Kahramanmaras) were obtained and one hundred three isolates were collected. Using the (GTG) 5 -PCR genomic fingerprint analysis method, seven of them were observed to be different and conventional tests of these isolates were performed. Molecular identification of two isolates carrying the bacteriocin gene and having antimicrobial activity by agar disc diffusion method was performed by 16S rRNA sequence analysis. As a result, the seven isolates were identified as Aerococcus urinaeequi (EK1), Streptococcus salivarius (EK2), Leuconostoc mesenteroides (EK3), Macrococcus caseolyticus (EK4), Lactococcus garvieae (EK5), Staphylococcus saprophyticus (EK6) and Lactobacillus sakei (EK7). Among these strains, it has been determined that Ln. mesenteroides and L. sakei carried the mecentericin and sacacin genes. When antimicrobial activity against different strains was examined, inhibition formations of Ln. mesenteroides and L. sakei on Enterococcus faecalis, Shigella dysenteriae and Escherichia coli O157: H7 were observed.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Bacteriocin Producing Bacteria Isolated from Turkish Traditional Sausage Samples

Erkaya¹,

Genç²,

Akbulut³

et al. 2020

J Pure Appl Microbiol

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show abstract

“…Currently, the only way to determine the sensitivity of a bacteria species to IR is to conduct extensive experimentation, requiring both the ability to culture the bacteria of interest and a high-powered source of IR. While survival screens have enabled the isolation of highly radioresistant bacteria (30, 51) (52) no such discovery studies have been conducted for radiosensitive bacteria. As an example of applying TolRad to screen existing proteomes, we applied TolRad to a collection of 152 proteomes downloaded from UniPro, including the ATCC Human Microbiome strain collection ( Sup.…”

Section: Discussionmentioning

confidence: 99%

TolRad: A model for predicting radiation tolerance using Pfam annotations identifies novel radiosensitive bacterial species from reference genomes and MAGs

Sweet,

Burroughs,

Jang

et al. 2023

Preprint

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The trait of ionizing radiation (IR) tolerance is variable between bacterial species, with radiosensitive bacteria succumbing to acute doses around 100Gy and extremophiles able to survive doses exceeding 10,000Gy. While survival screens have identified multiple highly radioresistant bacteria, such systemic searches have not been conducted for radiosensitive bacteria. The taxonomy-level diversity of IR of intolerance across bacteria is poorly understood, as are genetic elements that influence IR sensitivity. Using the protein domain frequencies from 61 bacterial species with experimentally determined IR D10 values (the dose at which only 10% of the population survives) we trained TolRad, a random forest binary classifier, to distinguish between radiosensitive bacteria (D10 < 200Gy) and radiation tolerant bacteria (D10 > 200Gy). On the hidden species, TolRad had an accuracy of 0.900. We applied TolRad to 152 UniProt-hosted bacterial proteomes, including 37 strains from the ATCC Human Microbiome Collection, and classified 34 species as radiosensitive. Whereas IR intolerance (D10 < 200Gy) in the training dataset had been confined to the phylumProteobacterium, this initial TolRad screen identified radiosensitive bacteria in 2 additional phyla. We experimentally validated the predicted radiosensitivity of a key species of the human microbiome from theBacteroidotaphyla. To demonstrate that TolRad can be applied to Metagenome-Assembled Genome (MAGs), we tested the accuracy of TolRad on Egg-NOG assembled proteomes (0.965) and partial proteomes. Finally, three collections of MAGs were screened using TolRad, identifying further phylum with radiosensitive species and suggesting that environmental conditions influence the abundance of radiosensitive bacteria.ImportanceBacterial species have vast genetic diversity, allowing for life in extreme environments and the conduction of complex chemistry. The ability to harness the full potential of bacterial diversity is hampered by the lack of high-throughput experimental or bioinformatic methods for characterizing bacterial traits. Here, we present a computational model that usesde novogenerated genome annotations to classify a bacterium as tolerant of ionizing radiation (IR) or as radiosensitive. This model allows for rapid screening of bacterial communities for low-tolerance species that are of interest for both mechanistic studies into bacterial sensitivity to IR and biomarkers of IR exposure.

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Radiophilic, Radioresistant, and Radiotolerant Microorganisms

Kanekar¹,

Kanekar²

2022

Diversity and Biotechnology of Extremophilic Microorganisms From India

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Radiation-resistant Macrococcus caseolyticus (A) isolated from radiation-processed semidried prawns

Cited by 5 publications

References 19 publications

Bacteriocin Producing Bacteria Isolated from Turkish Traditional Sausage Samples

Bacteriocin Producing Bacteria Isolated from Turkish Traditional Sausage Samples

TolRad: A model for predicting radiation tolerance using Pfam annotations identifies novel radiosensitive bacterial species from reference genomes and MAGs

Radiophilic, Radioresistant, and Radiotolerant Microorganisms

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