Radiation induces submandibular gland damage by affecting Cdkn1a expression and regulating expression of miR-486a-3p in a xerostomia mouse model

Wang, Wei; Xiao, Caizhi; Chen, Hong; Li, Fangfei; Xia, Dongqin

doi:10.17219/acem/136457

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…Permanent xerostomia can also occur due to SGs losing their secretory acinar cells [11]. About two-thirds of the volume of unstimulated saliva is contributed by the submandibular glands (SMGs) [12], IR-induced damage in SMGs is common [13].…”

Section: Introductionmentioning

confidence: 99%

Effect of silencing SSB1 gene on the expression of NBS1 in irradiated rat submandibular gland cells

Linjing Gao,

Qiuli Lv,

Yude Huang

et al. 2024

Cell Mol Biol (Noisy-le-grand)

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The salivary gland (SG) is a kind of organ vulnerable to ionizing radiation(IR). Radiotherapy is an important treatment for head and neck cancer, but in the process of radiotherapy, salivary gland cells will be injured by radiation to a certain extent. Ionising radiation-induced DNA double-strand break (IR-DSBs) is one of the most serious DNA damage. DNA repair proteins such as Nijmegen breakage syndrome protein 1 (NBS1) play a key role in the identification and repair of DNA damage, but the interaction between single-stranded DNAbinding protein 1(SSB1) and NBS1 has not been elucidated. In this study, we irradiated rat submandibular gland (SMG) cells, which were either infected with a rAdE5-SSB1-1p2-shRNA recombinant adenovirus to silence SSB or a control virus, to explore the effect of IR on the expression of NBS1 in the absence of SSB. Our results showed that the SSB1 mRNA transcripts and protein expression of SSB1 and NBS1 initially increased and decreased later with increased doses. The relative expression reached the highest levels when the SMG cells were irradiated with 2Gy of IR. Silencing the SSB1 gene suppressed the expression of both SSB1 and NBS1 regardless of irradiation. The expression of NBS1 decreased when the SSB1 gene was silenced. We concluded that IR affected the expression of both SSB1 and NBS1 and there is a synergistic effect on IR-induced NBS1 suppression and DSBs repair in SMG cells. These observations shed light on further investigation and elucidation of IR-caused DNA repair mechanisms.

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Section: Introductionmentioning

confidence: 99%

Effect of silencing SSB1 gene on the expression of NBS1 in irradiated rat submandibular gland cells

Linjing Gao,

Qiuli Lv,

Yude Huang

et al. 2024

Cell Mol Biol (Noisy-le-grand)

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“…Examples of papers adhering to respective checklists, published in Advances in Clinical and Experimental Medicine in 2021 and 2022[46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63][64][65] …”

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confidence: 99%

Checklists for reporting research in Advances in Clinical and Experimental Medicine: How to choose a proper one for your manuscript?

Misiak¹,

Kurpas²

2022

Adv Clin Exp Med

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Various guidelines for authors of research papers and the checklists that often accompany these statements play an important role in the creation of carefully written scientific papers -for authors, they serve as tools to ensure the correct structure and content of the manuscript, increasing the chances that a paper will be published in a journal with a high rejection rate. The aim of this editorial is to provide a concise outline of the checklists most frequently used to guide the structuring of papers published in Advances in Clinical and Experimental Medicine, and to support current and prospective authors of this journal in choosing a checklist for their manuscript.The EQUATOR website is presented as a useful tool in choosing a checklist: https://www.equator-network.org/. Then, 8 checklists that are most popular among authors who publish their work in Advances in Clinical and Experimental Medicine are outlined: STROBE -for observational studies; ARRIVE -for any area of bioscience research using laboratory animals; CASP -for qualitative studies; CONSORT -for parallel group randomized trials; PRISMA -for all reviews and meta-analyses; SQUIRE -for studies on quality improvement in healthcare; STARD -for diagnostic accuracy studies; REMARK -for tumor marker prognostic studies. Each of the 8 presented checklists is discussed in a following order: 1) the name of the checklist is explained; 2) the type of articles to which it is intended is pointed out; 3) the structure of the checklist is explained; 4) if there are any extensions of the presented checklist for specific subtypes of papers, they are listed; 5) the most important literature on the presented checklist is provided.As a take-home message, basic tips for choosing a checklist are formulated. Finally, examples of papers adhering to each discussed checklist are provided.

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Radiation induces submandibular gland damage by affecting Cdkn1a expression and regulating expression of miR-486a-3p in a xerostomia mouse model

Abstract: Radiation induces submandibular gland damage by affecting Cdkn1a expression and regulating expression of miR-486a-3p in a xerostomia mouse model.

Cited by 2 publications

References 22 publications

Effect of silencing SSB1 gene on the expression of NBS1 in irradiated rat submandibular gland cells

Effect of silencing SSB1 gene on the expression of NBS1 in irradiated rat submandibular gland cells

Checklists for reporting research in Advances in Clinical and Experimental Medicine: How to choose a proper one for your manuscript?

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