Radiation-Induced Lung Response of AcB/BcA Recombinant Congenic Mice

Genetic Deficiency in Complement Component 4b Does Not Alter Radiation-Induced Lung Disease in Mice

“…3) and is in agreement with previous reports of the wild-type strain (5)(6)(7)(8)(9)(10). Image analysis of histological sections demonstrated that C4b -/-mice also developed significant fibrosis after thoracic irradiation, but was similar to the level observed in wildtype mice (Fig.…”

Combined Tlr2 and Tlr4 Deficiency Increases Radiation-Induced Pulmonary Fibrosis in Mice

“…Animal models which exhibit radiation-induced alveolitis and fibrosis phenotypes with similar temporal and histological patterns to the clinical response (1,2) also show an increase in lavage neutrophils (5)(6)(7) and tissue mast cell levels (8). Specifically, we (8)(9)(10) and others (11,12) have reported that C57BL/6J (B6) mice respond to high-dose whole-thorax irradiation by developing alveolitis and atelectatic regions of fibrosis at approximately 6 months after radiation treatment.…”

“…Lung damage was elicited by whole-thorax radiation exposure using a Gamma cell Cesium-137 unit as previously described (8,10). A dose of 18 Gy delivered at 0.6 Gy/min was given to elicit a lung disease response in the majority of the animals based on the known response of the background strain (7-12, 18, 20, 21).…”

“…At necropsy, bronchoalveolar lavage was performed as previously described (8,10). The lungs were then removed, and the single left lobe of each mouse was perfused with 10% neutral buffered formalin and submitted for histological processing.…”

“…Inflammatory cell counts were performed (Â400 magnification) on cytocentrifuged cells (214.2 g for 3 min), after staining with a hematoxylin-and-eosin kit (Hema-3 Stain Set by Protocol) as previously described (8,10).…”