Radiation-induced biomimetic modification of dual-layered nano/microfibrous scaffolds for vascular tissue engineering

Surface modification and endothelialization of biomaterials as potential scaffolds for vascular tissue engineering applications

“…The gelatin modified microfibrous layer exhibited the proliferation and infiltration of SMCs owing to its large pores and coupled gelatin, while the nanofibrous layer accelerated proliferation of ECs. 401 Thus, these specially designed dual-layered scaffolds can alternatively mimic native blood vessels for use in vascular tissue engineering. Choong et al used ATRP to graft glycidyl methacrylate onto the PCL surface to introduce epoxy side groups and subsequently covalently immobilize gelatin.…”

Development and characterization of heparin-immobilized polycaprolactone nanofibrous scaffolds for tissue engineering using gamma-irradiation

“…The goal of this work was threefold: (1) to prepare by deposition PCL nanobers generated by electrospinning equipment, (2) to determine the capacity for cell adhesion and proliferation on electrospun and methacrylated PCL scaffolds, which would subsequently be radiation-graed, and (3) to incorporate heparin into the scaffolds to provide sustained release of growth factors. [29][30][31] PCL nanobers were prepared by electrospinning and then AEMA was graed onto the PCL scaffolds to gra amino groups using gamma-irradiation. Aer AEMA-graing, heparin was then immobilized by constituting amide bonds between the amino groups of AEP and the carboxylic acid groups of heparin using the EDC/NHS reaction.…”

Engineered ECM-like microenvironment with fibrous particles for guiding 3D-encapsulated hMSC behaviours

“…In particular, the RGD peptide has been shown to regulate cell adhesion, spreading on the substrates, and a series of prolonged behaviours such as proliferation or differentiation. [21][22][23] To nd the biological function of the R-FP incorporated hydrogel, we encapsulated the hMSC in the hydrogel and measured the cell viability for 14 days. The cytotoxicity of the R-FP itself was investigated in a previous study, in which the R-FP did not show any harmful effect up to 300 mg mL À1 .…”

“…20 It not only generated random or aligned brous meshes, but it also produced meshes with nanoto micron bre diameter. 21,22 However, most of the electrospun nanobers have relatively small pores that restrict cell inltration into the nanobers. Furthermore, the 3D scaffolding function has only partially been achieved.…”